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Literature summary for 4.2.1.84 extracted from
- Aliphatic nitrile hydratase from Arthrobacter sp. J-1. Purification and characterization (1982), Agric. Biol. Chem., 46, 1165-1174.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glycine-NaOH | activates | Arthrobacter sp. |  |
General Stability
| General Stability | Organism |
|---|---|
| potassium phosphate buffer has a negative effect on stability | Arthrobacter sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 1 mM of AgNO3 | Arthrobacter sp. | |
| BaCl2 | weak inhibition | Arthrobacter sp. | |
| glutaronitrile | - |
Arthrobacter sp. | |
| Hg2+ | complete inhibition at 0.01 mM Hg2+ | Arthrobacter sp. | |
| iodoacetic acid | - |
Arthrobacter sp. | |
| Isobutyronitrile | - |
Arthrobacter sp. | |
| KCN | complete inhibition at 0.1 mM, competitive with acetonitrile as substrate | Arthrobacter sp. | |
| p-chloromercuribenzoate | strong inhibition at 1 mM | Arthrobacter sp. | |
| sulfhydryl reagents | - |
Arthrobacter sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.88 | - |
acrylonitrile | pH 7, 30°C | Arthrobacter sp. | |
| 1.9 | - |
Propionitrile | pH 7, 30°C | Arthrobacter sp. | |
| 5.78 | - |
acetonitrile | pH 7, 30°C | Arthrobacter sp. | |
| 8.77 | - |
Methacrylonitrile | pH 7, 30°C | Arthrobacter sp. | |
| 10.8 | - |
n-butyronitrile | pH 7, 30°C | Arthrobacter sp. | |
| 10.9 | - |
Chloroacetonitrile | pH 7, 30°C | Arthrobacter sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Arthrobacter sp. | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000 + x * 27000, SDS-disc gel electrophoresis | Arthrobacter sp. |
| 27000 | - |
x * 24000 + x * 27000, SDS-disc gel electrophoresis | Arthrobacter sp. |
| 420000 | - |
gel filtration | Arthrobacter sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. | - |
- |
- |
| Arthrobacter sp. J-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, using several chromatographic steps, including DEAE, hydroxyapatite, and Sephadex G-200 column chromatography | Arthrobacter sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.17 | - |
- |
Arthrobacter sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7.0, 50% glycerol, stable for at least 40 days, 40% loss of activity after 6 months | Arthrobacter sp. |
| 0°C, pH 7.0, 50% loss of activity after 40 days | Arthrobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetonitrile + H2O | - |
Arthrobacter sp. | acetamide | - |
ir | |
| acetonitrile + H2O | - |
Arthrobacter sp. J-1 | acetamide | - |
ir | |
| acrylonitrile + H2O | - |
Arthrobacter sp. | 2-propenoic acid amide | i.e. acrylic acid amide | r | |
| chloroacetonitrile + H2O | - |
Arthrobacter sp. | chloroacetamide | - |
ir | |
| chloroacetonitrile + H2O | - |
Arthrobacter sp. J-1 | chloroacetamide | - |
ir | |
| hydroxyacetonitrile + H2O | - |
Arthrobacter sp. | hydroxyacetamide | - |
r | |
| methacrylonitrile + H2O | - |
Arthrobacter sp. | methylacrylic acid amide | - |
r | |
| additional information | aliphatic nitriles with more than 5 carbons and aromatic nitriles cannot act as substrate | Arthrobacter sp. | ? | - |
? | |
| additional information | aliphatic nitriles with more than 5 carbons and aromatic nitriles cannot act as substrate | Arthrobacter sp. J-1 | ? | - |
? | |
| n-butyronitrile + H2O | - |
Arthrobacter sp. | n-butyric acid amide | - |
r | |
| n-butyronitrile + H2O | - |
Arthrobacter sp. J-1 | n-butyric acid amide | - |
r | |
| propionitrile + H2O | - |
Arthrobacter sp. | propionic acid amide | i.e. propionamide | r | |
| propionitrile + H2O | - |
Arthrobacter sp. J-1 | propionic acid amide | i.e. propionamide | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000 + x * 27000, SDS-disc gel electrophoresis | Arthrobacter sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Arthrobacter sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
10 min, 20% loss of activity | Arthrobacter sp. |
| 35 | - |
1 hour, 10 mM methacrylonitrile stable up to this temperature | Arthrobacter sp. |
| 55 | - |
10 min, nearly complete inactivation | Arthrobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.2 | - |
Arthrobacter sp. |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
KCN | pH 7, 30°C | Arthrobacter sp. | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arthrobacter sp. | - |
- |
3.6 |
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