EC Number   |
Activating Compound |
Reference |
|---|
   3.2.1.7 | inulin |
with raw inulin in bioreactor, progressive increase in inulinase production with increase in inulin concentration up to 4.0% with a maximum inulinase activity of 50.2 IU/ml |
678762 |
| 3.2.1.7 | K+ |
5 mM, 113% of initial activity |
729526 |
| 3.2.1.7 | more |
high endoinulinase productivity of 9.9 U/ml when grown in a liquid medium containing inulin as the carbon source at 30°C for 4 days |
695820 |
| 3.2.1.7 | more |
inulinase yield in bioreactor is almost 6times higher than basal medium used in shake flask. Tween-60 has no significant effect. Inoculum of 12 h old yeast culture is optimum for inulinase activity (36.9 IU/ml). 5% of 12 h old culture is optimum for the production of inulinase in the medium. Progressive increase in inulinase activity up to 37.0 IU/ml as the agitation speed is increased to 150 rpm. Maximum production of inulinase (40.2 IU/ml) after 60 h of incubation |
678762 |
| 3.2.1.7 | more |
maximum inulinase production (55.4 U/ml) by strain YS-1 is obtained at an agitation rate of 200 rpm and aeration of 0.75 volume/volume per minute in a stirred tank reactor (1.5 l) with a fermentation time of 60 h or inulinase activity of 50.2 U/ml is obtained from the same yeast strain under agitation (200 rpm) and aeration (0.75 volume/volume per minute) at 30°C after 60 h of fermentation in 1.5 l of fermentor when raw inulin (4.0%) from root tubers of Asparagus officinalis is used as the production medium. When strain NRRL Y-7571 is grown in the culture containing sugarcane bagasse as support and carbon source and corn steep liquor as nitrogen supplement, the extracellular inulinase concentration reaches 391.9 U/g of dry fermented bagasse. The use of soybean bran decreases the time to reach the maximum activity from 96 to 24 h and the maximum productivity achieved strain NRRL Y-7571 is 8.87 U/gds per h |
695820 |
| 3.2.1.7 | more |
no effect by 1 mM DTT on wild-type enzyme activity |
750169 |
| 3.2.1.7 | more |
the fungus shows good growth on a medium containing 40% (v/v) of dandelion tap root extract composed of 50 g tap roots blended with 200 ml water and 2% yeast extract medium and produces 55 U/ml of inulinase activity in 96 h at 30°C and 150 rpm during the liquid fermentation. Dandelion tap root extract in the liquid medium induces the endoinulinase synthesis |
695820 |
| 3.2.1.7 | SDS |
activates the wild-type enzyme by 62% at 0.1% v/v |
750169 |
| 3.2.1.7 | Sodium dodecyl sulfate |
0.001% is most effective for the inulinase activity (36.9 IU/ml) |
678762 |
| 3.2.1.7 | Triton X-100 |
activates the wild-type enzyme by 33% at 0.1% v/v |
750169 |
