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Literature summary for 3.2.1.7 extracted from
- Inulinase-expressing microorganisms and applications of inulinases (2009), Appl. Microbiol. Biotechnol., 82, 211-220.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | high endoinulinase productivity of 9.9 U/ml when grown in a liquid medium containing inulin as the carbon source at 30°C for 4 days | Penicillium sp. | |
| additional information | maximum inulinase production (55.4 U/ml) by strain YS-1 is obtained at an agitation rate of 200 rpm and aeration of 0.75 volume/volume per minute in a stirred tank reactor (1.5 l) with a fermentation time of 60 h or inulinase activity of 50.2 U/ml is obtained from the same yeast strain under agitation (200 rpm) and aeration (0.75 volume/volume per minute) at 30°C after 60 h of fermentation in 1.5 l of fermentor when raw inulin (4.0%) from root tubers of Asparagus officinalis is used as the production medium. When strain NRRL Y-7571 is grown in the culture containing sugarcane bagasse as support and carbon source and corn steep liquor as nitrogen supplement, the extracellular inulinase concentration reaches 391.9 U/g of dry fermented bagasse. The use of soybean bran decreases the time to reach the maximum activity from 96 to 24 h and the maximum productivity achieved strain NRRL Y-7571 is 8.87 U/gds per h | Kluyveromyces marxianus | |
| additional information | the fungus shows good growth on a medium containing 40% (v/v) of dandelion tap root extract composed of 50 g tap roots blended with 200 ml water and 2% yeast extract medium and produces 55 U/ml of inulinase activity in 96 h at 30°C and 150 rpm during the liquid fermentation. Dandelion tap root extract in the liquid medium induces the endoinulinase synthesis | Aspergillus niger |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | yeasts can produce more inulinase than bacteria | Kluyveromyces marxianus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed on the cell surface of Saccharomyces cerevisiae EBY100 cells | Pseudomonas mucidolens |
| INU2 gene encoding endoinulinase expressed in SUC2-deleted Saccharomyces cerevisiae | Aspergillus ficuum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | - |
Penicillium sp. |  |
| Cu2+ | - |
Penicillium sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
inulin | - |
Penicillium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55500 | - |
sequence analysis | Talaromyces purpureogenus |
| 55800 | - |
sequence analysis | Aspergillus ficuum |
| 55900 | - |
sequence analysis | Aspergillus niger |
| 58800 | - |
sequence analysis | Kluyveromyces marxianus |
| 68000 | - |
- |
Penicillium sp. |
| 75000 | - |
- |
Arthrobacter sp. |
| 87900 | - |
sequence analysis | Arthrobacter sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. | Q9RBA5 | - |
- |
| Aspergillus ficuum | O94220 | - |
- |
| Aspergillus niger | A5ABL2 | strain NK-126, 12, 817 and TISTR 3570 | - |
| Aspergillus niger CBS 513.88 | A5ABL2 | strain NK-126, 12, 817 and TISTR 3570 | - |
| Kluyveromyces marxianus | P28999 | strains ATCC 16045, ATCC 52466, NRRL Y-7571, S120, YS-1, A1 and A2 | - |
| Penicillium sp. | - |
- |
- |
| Penicillium sp. TN-88 | - |
- |
- |
| Pseudomonas mucidolens | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
| Talaromyces purpureogenus | O00056 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | - |
Penicillium sp. | ? | - |
? | |
| inulin + H2O | - |
Kluyveromyces marxianus | ? | - |
? | |
| inulin + H2O | - |
Aspergillus niger | ? | - |
? | |
| inulin + H2O | - |
Arthrobacter sp. | ? | - |
? | |
| inulin + H2O | - |
Pseudomonas mucidolens | ? | - |
? | |
| inulin + H2O | - |
Penicillium sp. TN-88 | ? | - |
? | |
| inulin + H2O | - |
Aspergillus niger CBS 513.88 | ? | - |
? | |
| inulin + H2O | - |
Aspergillus ficuum | 1-kestose + ? | - |
? | |
| inulin + H2O | - |
Pseudomonas sp. | inulooligosaccharide | ranging from DP2 to DP7 | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoinulinase | - |
Pseudomonas sp. |
| endoinulinase | - |
Penicillium sp. |
| endoinulinase | - |
Talaromyces purpureogenus |
| endoinulinase | - |
Kluyveromyces marxianus |
| endoinulinase | - |
Aspergillus niger |
| endoinulinase | - |
Arthrobacter sp. |
| endoinulinase | - |
Aspergillus ficuum |
| endoinulinase | - |
Pseudomonas mucidolens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Penicillium sp. |
| 50 | - |
- |
Kluyveromyces marxianus |
| 50 | - |
- |
Arthrobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Penicillium sp. |
| 5.5 | - |
- |
Pseudomonas sp. |
| 7.5 | - |
- |
Arthrobacter sp. |
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