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Information on EC 6.5.1.4 - RNA 3'-terminal-phosphate cyclase (ATP)
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6.5.1.4 RNA 3'-terminal-phosphate cyclase (ATP)IUBMB Comments
The enzyme converts the 3'-terminal phosphate of various RNA substrates into the 2',3'-cyclic phosphodiester in an ATP-dependent reaction. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a histidine residue [5,6]. The adenylate group is then transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [RNA]-3'-(5'-diphosphoadenosine). Finally, the enzyme catalyses an attack of the vicinal O-2' on the 3'-phosphorus, which results in formation of cyclic phosphate and release of the adenylate. The enzyme also has a polynucleotide 5' adenylylation activity . cf. EC 6.5.1.5, RNA 3'-terminal-phosphate cyclase (GTP).
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Word Map
- 6.5.1.4
-
phosphodiester
-
cyclization
- ligases
-
nucleotidylation
-
adenylylation
- cyclases
- polynucleotide
- eucarya
- pre-rrna
- oligoribonucleotides
-
ribozyme
-
3',5'-phosphodiester
-
alpha-32patp
-
cyclase-like
-
2'-phosphate
The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
=
+
+
+
[RNA]-3'-(3'-phospho-ribonucleoside)
=
+
+
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
Synonyms
rcl1p, rna 3'-terminal phosphate cyclase, rna 3'-phosphate cyclase, rna cyclase, st-rtc, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
PH1529
RNA 3'-phosphate cyclase
RNA 3'-teminal phosphate cyclase
RNA 3'-terminal phosphate cyclase
RNA 3'-terminal phosphate cylase
RNA cyclase
-
-
-
-
RNA-3'-phosphate cyclase
Rtc
RtcA
RNA 3'-terminal phosphate cyclase
-
-
-
-
RNA-3'-phosphate cyclase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + [RNA 3'-phosphate cyclase]-L-histidine = [RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
(1a)
-
-
-
ATP + [RNA]-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside) = [RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
(1b)
-
-
-
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine) = [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
(1c)
-
-
-
ATP + [RNA]-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
formation of a covalent cyclase-AMP intermediate
-
ATP + [RNA]-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
overall reaction
-
-
-
ATP + [RNA]-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
upon binding of Mg2+ to residue E10, the triphosphate group of the trapped ATP changes its conformation, with help of ligands R17 and R39. The Nepsilon atom of H307 attacks the alpha-phosphate group to form a new P-N bond. When a truncated RNA is bound, its 3'-phosphate group may be forced to react with the phosphate group of AMP, and the activated 3'-phosphate group may be attacked by the 2'-hydroxyl group to generate the 2',3'-cyclic phosphodiester
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
esterification
-
-
cyclic phosphomonoester
-
Ligation
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
RNA-3'-phosphate:RNA ligase (cyclizing, AMP-forming)
The enzyme converts the 3'-terminal phosphate of various RNA substrates into the 2',3'-cyclic phosphodiester in an ATP-dependent reaction. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a histidine residue [5,6]. The adenylate group is then transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [RNA]-3'-(5'-diphosphoadenosine). Finally, the enzyme catalyses an attack of the vicinal O-2' on the 3'-phosphorus, which results in formation of cyclic phosphate and release of the adenylate. The enzyme also has a polynucleotide 5' adenylylation activity [7]. cf. EC 6.5.1.5, RNA 3'-terminal-phosphate cyclase (GTP).
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CAS REGISTRY NUMBER
COMMENTARY
85638-41-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + DNA 5'-terminal-phosphate
AMP + AppDNA
-
RtcA also catalyzes adenylylation of 5'-phosphate ends of DNA strands to form AppDNA product
-
-
?
ATP + oligodeoxyribonucleotide 3'-terminal phosphate
AMP + diphosphate + oligodeoxyribonucleotide-2',3'-cyclic-phosphate
500fold poorer substrate than oligoribonucleotide 3'-terminal phosphate
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic phosphate
-
RtcA catalyzes the synthesis of RNA 2',3'-cyclic phosphate ends via an ATP-dependent pathway comprising three nucleotidyl transfer steps: reaction of RtcA with ATP to form a covalent RtcA-(histidinyl-N)-AMP intermediate and release diphosphate, transfer of AMP from RtcA to an RNA 3'-phosphate to form an RNA(3')pp(5')A intermediate, and attack by the terminal nucleoside O2' on the 3'-phosphate to form an RNA 2',3'-cyclic phosphate product and release AMP
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphodiester
ATP + RNA 5'-terminal-phosphate
AMP + AppRNA
-
RtcA also catalyzes adenylylation of 5'-phosphate ends of RNA strands to form AppRNA product
-
-
?
ATP + tobacco mosaic virus RNA
AMP + ?
-
fragments modified by ligation of pGp, pAp, or pCp
-
?
ATP + [RNA 3'-phosphate cyclase]-L-histidine
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
ATPgammaS + RNA 3'-terminal-phosphate
AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate
RNA-N3'p + ATP
RNA-N-2',3'-cyclic phosphate + AMP + diphosphate
-
-
-
-
ir
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside)
[RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
ATP + 5S rRNA
AMP + ?
-
modified by ligation of [5'-P]pCp to the 3'-terminus
-
?
ATP + AAAAUAAAAGpCp
AMP + diphosphate + AAAAUAAAAGpC-2',3'-cyclic phosphate
-
-
-
ir
ATP + AAAAUAAAAGpCp
AMP + diphosphate + AAAAUAAAAGpC-2',3'-cyclic phosphate
-
-
-
-
ir
ATP + human U14 snoRNA
AMP + ?
-
modified by ligation of [5'-P]pCp to the 3'-terminus
-
?
ATP + RNA 2'-terminal-phosphate
?
-
the rate of 2'-phosphate cyclization by RtcA is five orders of magnitude slower than 3'-phosphate cyclization, notwithstanding that RtcA binds with similar affinity to RNA3'p and RNA2'p substrates
-
-
?
ATP + RNA 3'-terminal-phosphate
?
the enzyme could be involved in the maintenance of cyclic ends in tRNA splicing intermediates or in the cyclization of the 3' end of U6 sbRNA
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
RtcA can quantitatively cyclize 8-mer, 6-mer, and 4-mer RNA3'p substrates
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
no activity with dATP
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
ir
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
(Up)10Gp is cyclized at a faster rate than tRNAApGp or AUGp
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
human U6 small nuclear RNA
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
oligoribonucleotides
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphodiester
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphodiester
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphodiester
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphodiester
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphodiester
-
-
-
?
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphodiester
-
-
-
?
ATP + [RNA 3'-phosphate cyclase]-L-histidine
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
-
-
-
-
?
ATP + [RNA 3'-phosphate cyclase]-L-histidine
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
-
-
-
-
?
ATP + [RNA 3'-phosphate cyclase]-L-histidine
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
-
-
-
-
?
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
-
-
-
-
?
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
-
-
-
-
?
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
-
-
-
-
?
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
-
-
-
-
?
ATPgammaS + RNA 3'-terminal-phosphate
AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
-
?
ATPgammaS + RNA 3'-terminal-phosphate
AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate
-
ir
-
?
CTP + RNA 3'-terminal-phosphate
CMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
-
?
CTP + RNA 3'-terminal-phosphate
CMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
reaction proceeds 15-20 times slower than in presence of ATP
-
-
?
GTP + RNA 3'-terminal-phosphate
GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
-
?
GTP + RNA 3'-terminal-phosphate
GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
-
?
GTP + RNA 3'-terminal-phosphate
GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
reaction proceeds 15-20 times slower than in presence of ATP
-
-
?
UTP + RNA 3'-terminal-phosphate
UMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
-
-
-
?
UTP + RNA 3'-terminal-phosphate
UMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
reaction proceeds 15-20 times slower than in presence of ATP
-
-
?
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside)
[RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
-
-
-
-
?
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside)
[RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
-
-
-
-
?
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside)
[RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
-
-
-
-
?
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
-
-
-
-
?
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
-
-
-
-
?
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
-
-
-
-
?
additional information
?
-
-
nucleoside 3'-monophosphates and nucleoside 5',3'-bisphosphates are no substrates
-
?
additional information
?
-
nonessential as demonstrated by knockout experiments
-
?
additional information
?
-
-
nonessential as demonstrated by knockout experiments
-
?
additional information
?
-
precise function is not known, may be responsible for generating or regenerating cyclic phosphate RNA ends required by RNA ligases
-
?
additional information
?
-
-
precise function is not known, may be responsible for generating or regenerating cyclic phosphate RNA ends required by RNA ligases
-
?
additional information
?
-
-
RtcA readily adenylylates the 5'-phosphate at a 5'-PO4/3'-OH nick in duplex DNA but is unable to covert the nicked DNA-adenylate to a sealed phosphodiester
-
-
?
additional information
?
-
-
the enzyme is incapable of phosphodiester synthesis (i.e., ligation) following adenylation of the 5'-terminal phosphate
-
-
?
additional information
?
-
nonessential as demonstrated by knockout experiments
-
?
additional information
?
-
-
trinucleotides are the shortest oligonucleotides able to act as substrates, ribonucleoside 3'-phosphates and ribonucleoside 5',3'-phosphates are no substrates
-
?
additional information
?
-
-
the enzyme is incapable of phosphodiester synthesis (i.e., ligation) following adenylation of the 5'-terminal phosphate
-
-
?
additional information
?
-
-
the enzyme is also able to convert DNA-3'-phosphate to single-stranded DNA-3'-diphosphate-5'-adenylate but not able to modify a 3'-phosphate in nicked double-stranded DNA
-
-
?
additional information
?
-
the enzyme recognizes substrate RNA by ensuring that the terminal 3'-phosphate makes a large contribution to RNA binding
-
-
?
additional information
?
-
-
the enzyme recognizes substrate RNA by ensuring that the terminal 3'-phosphate makes a large contribution to RNA binding
-
-
?
additional information
?
-
the enzyme recognizes substrate RNA by ensuring that the terminal 3'-phosphate makes a large contribution to RNA binding
-
-
?
additional information
?
-
-
it was suggested that the enzyme could be responsible for the generation of the 2',3'-cyclic termini required for ligation of RNA by eukaryotic ligases and, consequently, be involved in tRNA slicing. It was found, however, that the slicing endonucleases responsible for the excision of introns from tRNA precursors in yeast and Xenopus sp. directly produce RNA molecules with cyclic phosphate ends
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + [RNA 3'-phosphate cyclase]-L-histidine
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside)
[RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
ATP + RNA 3'-terminal-phosphate
?
the enzyme could be involved in the maintenance of cyclic ends in tRNA splicing intermediates or in the cyclization of the 3' end of U6 sbRNA
-
-
?
ATP + [RNA 3'-phosphate cyclase]-L-histidine
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
-
-
-
-
?
ATP + [RNA 3'-phosphate cyclase]-L-histidine
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
-
-
-
-
?
ATP + [RNA 3'-phosphate cyclase]-L-histidine
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + diphosphate
-
-
-
-
?
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
-
-
-
-
?
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
-
-
-
-
?
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
-
-
-
-
?
ATP + [RNA]-3'-(3'-phospho-ribonucleoside)
AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
-
-
-
-
?
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside)
[RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
-
-
-
-
?
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside)
[RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
-
-
-
-
?
[RNA 3'-phosphate cyclase]-Ntau-(5'-adenylyl)-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside)
[RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
-
-
-
-
?
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
-
-
-
-
?
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
-
-
-
-
?
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoadenosine)
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + AMP
-
-
-
-
?
additional information
?
-
nonessential as demonstrated by knockout experiments
-
?
additional information
?
-
-
nonessential as demonstrated by knockout experiments
-
?
additional information
?
-
precise function is not known, may be responsible for generating or regenerating cyclic phosphate RNA ends required by RNA ligases
-
?
additional information
?
-
-
precise function is not known, may be responsible for generating or regenerating cyclic phosphate RNA ends required by RNA ligases
-
?
additional information
?
-
nonessential as demonstrated by knockout experiments
-
?
additional information
?
-
-
it was suggested that the enzyme could be responsible for the generation of the 2',3'-cyclic termini required for ligation of RNA by eukaryotic ligases and, consequently, be involved in tRNA slicing. It was found, however, that the slicing endonucleases responsible for the excision of introns from tRNA precursors in yeast and Xenopus sp. directly produce RNA molecules with cyclic phosphate ends
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ATP
ATP-dependent conversion of the 3'-phosphate to the 2',3'-cyclic phosphodiester
ATP
-
requires ATP as cofactor, other ribonucleoside triphosphates are used very inefficiently
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
additional information
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Colorectal Neoplasms
Next-generation sequencing analysis of multiplex families with atypical psychosis.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE