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Literature summary for 6.5.1.4 extracted from

  • Das, U.; Shuman, S.
    2-Phosphate cyclase activity of RtcA: a potential rationale for the operon organization of RtcA with an RNA repair ligase RtcB in Escherichia coli and other bacterial taxa (2013), RNA, 19, 1355-1362.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
H309A mutant is unreactive with either 3'-phosphate RNA end or 2'-phosphate RNA end Escherichia coli
H320A mutant is unreactive with either 3'-phosphate RNA end or 2'-phosphate RNA end Homo sapiens

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Escherichia coli
using Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA 2'-terminal-phosphate
-
 Homo sapiens ?
-
 ?
ATP + RNA 2'-terminal-phosphate the rate of 2'-phosphate cyclization by RtcA is five orders of magnitude slower than 3'-phosphate cyclization, notwithstanding that RtcA binds with similar affinity to RNA3'p and RNA2'p substrates Escherichia coli ?
-
 ?
ATP + RNA 3'-terminal-phosphate
-
 Homo sapiens AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
ATP + RNA 3'-terminal-phosphate RtcA can quantitatively cyclize 8-mer, 6-mer, and 4-mer RNA3'p substrates Escherichia coli AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Rtc1
-
 Homo sapiens
RtcA
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 ? the rate of 2'-phosphate cyclization by RtcA is five orders of magnitude slower than 3'-phosphate cyclization, notwithstanding that RtcA binds with similar affinity to RNA3'p and RNA2'p substrates Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Escherichia coli
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
metabolism RtcA might serve an end healing function in an RNA repair pathway, by converting RNA 2'-phosphates, which cannot be spliced by RtcB, to 2',3'-cyclic phosphates that can be sealed Escherichia coli