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Literature summary for 6.5.1.4 extracted from

  • Tanaka, N.; Smith, P.; Shuman, S.
    Structure of the RNA 3-phosphate cyclase-adenylate intermediate illuminates nucleotide specificity and covalent nucleotidyl transfer (2010), Structure, 18, 449-457.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Codon Plus (DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
D287A activity very low, 1% of wild-type enzyme Escherichia coli
E270A reduced activity compared to wild-type enzyme, about 80% Escherichia coli
F135A activity very low, 3% of wild-type enzyme Escherichia coli
F251A considerably reduced activity compared to wild-type enzyme, about 30% Escherichia coli
H309A activity very low, less than 10% of wild-type enzyme Escherichia coli
H309G enzyme inactive Escherichia coli
P131G considerably reduced activity compared to wild-type enzyme, about 35% Escherichia coli
Q104A almost same activiy like wild-type enzyme Escherichia coli
Q288A activity very low, 2% of wild-type enzyme Escherichia coli
S129A almost same activiy like wild-type enzyme Escherichia coli
Y284A considerably reduced activity compared to wild-type enzyme (12%) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P46849
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AAAAUAAAAGpCp
-
Escherichia coli AMP + diphosphate + AAAAUAAAAGpC-2',3'-cyclic phosphate
-
ir

Subunits

Subunits Comment Organism
homodimer the apoenzyme of Escherichia coli RtcA crystallizes as a disulfide-linked homodimer and reveales a fold composed of four tandem modules, each comprising a four-stranded beta sheet overlying two alpha helices, X-ray diffraction Escherichia coli

Synonyms

Synonyms Comment Organism
RNA 3'-terminal phosphate cyclase
-
Escherichia coli
RtcA
-
Escherichia coli