Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Codon Plus (DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D287A | activity very low, 1% of wild-type enzyme | Escherichia coli |
E270A | reduced activity compared to wild-type enzyme, about 80% | Escherichia coli |
F135A | activity very low, 3% of wild-type enzyme | Escherichia coli |
F251A | considerably reduced activity compared to wild-type enzyme, about 30% | Escherichia coli |
H309A | activity very low, less than 10% of wild-type enzyme | Escherichia coli |
H309G | enzyme inactive | Escherichia coli |
P131G | considerably reduced activity compared to wild-type enzyme, about 35% | Escherichia coli |
Q104A | almost same activiy like wild-type enzyme | Escherichia coli |
Q288A | activity very low, 2% of wild-type enzyme | Escherichia coli |
S129A | almost same activiy like wild-type enzyme | Escherichia coli |
Y284A | considerably reduced activity compared to wild-type enzyme (12%) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P46849 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AAAAUAAAAGpCp | - |
Escherichia coli | AMP + diphosphate + AAAAUAAAAGpC-2',3'-cyclic phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homodimer | the apoenzyme of Escherichia coli RtcA crystallizes as a disulfide-linked homodimer and reveales a fold composed of four tandem modules, each comprising a four-stranded beta sheet overlying two alpha helices, X-ray diffraction | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
RNA 3'-terminal phosphate cyclase | - |
Escherichia coli |
RtcA | - |
Escherichia coli |