anaerobic ethylbenzene metabolism in the betaproteobacterium Aromatoleum aromaticum is initiated by anaerobic oxidation to acetophenone via (S)-1-phenylethanol. The subsequent carboxylation of acetophenone to benzoylacetate is catalyzed by an acetophenone-induced enzyme
the enzyme shows uncoupled ATPase activity with either bicarbonate or acetophenone in the absence of the second substrate. These observations indicate that both substrates may be phosphorylated. Purified enzyme shows no activity with GTP, CTP, UTP or ITP
anaerobic ethylbenzene metabolism in the betaproteobacterium Aromatoleum aromaticum is initiated by anaerobic oxidation to acetophenone via (S)-1-phenylethanol. The subsequent carboxylation of acetophenone to benzoylacetate is catalyzed by an acetophenone-induced enzyme
the enzyme shows uncoupled ATPase activity with either bicarbonate or acetophenone in the absence of the second substrate. These observations indicate that both substrates may be phosphorylated. Purified enzyme shows no activity with GTP, CTP, UTP or ITP
anaerobic ethylbenzene metabolism in the betaproteobacterium Aromatoleum aromaticum is initiated by anaerobic oxidation to acetophenone via (S)-1-phenylethanol. The subsequent carboxylation of acetophenone to benzoylacetate is catalyzed by an acetophenone-induced enzyme
anaerobic ethylbenzene metabolism in the betaproteobacterium Aromatoleum aromaticum is initiated by anaerobic oxidation to acetophenone via (S)-1-phenylethanol. The subsequent carboxylation of acetophenone to benzoylacetate is catalyzed by an acetophenone-induced enzyme
the enzyme contains 1 Zn2+ ion per alphabetagammadelta core complex. In spite of the presence of Zn in the enzyme, it is strongly inhibited by Zn2+ ions
the enzyme contains 1 Zn2+ ion per alphabetagammadelta core complex. In spite of the presence of Zn in the enzyme, it is strongly inhibited by Zn2+ ions. 0.2 mM ZnCl2: 50% inhibition of ATPase activity and carboxylation activity
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CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
structure of the Apc(alphaalpha'betagamma)2 core complex, to 3.0 A resolution. Subunit Apcbeta contains a domain fold composed of two beta-sheets in a barrel-like arrangement running into a bundle of eight short polyproline (type II)-like helical segments. Apcalpha and Apcalpha' possess ATP-binding modules of the ASKHA superfamily and presumably operate as ATP-dependent kinases for acetophenone and bicarbonate, respectively
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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
during purification, acetophenone carboxylase dissociates into inactive subcomplexes consisting of the 70-, 15-, 87-, and 75-kDa subunits (apcABCD gene products) and the 34-kDa subunit (apcE gene product), respectively. Acetophenone carboxylase activity is restored by mixing the purified subcomplexes
acetophenone carboxylase is formed in ethylbenzene- and toluene-adapted cells; expression of alpha-subunit is proportional to the applied acetophenone concentrations; the enzyme is synthesized at high levels in cells grown on ethylbenzene or acetophenone, but not in cells grown on benzoate
construction of a reporter strain by inserting a gene fusion of apcA, the first gene of the apc-bal operon, with the gene for the fluorescent protein mCherry into the chromosome. mCherry production is proportional to the applied acetophenone concentrations. The reporter strain allows quantification of acetophenone within a concentration range of 50 microM (detection limit) to 250 microM after 12 and 24 h. Production of the Apc-mCherry fusion protein in the reporter strain is highly specific and responds to acetophenone and both enantiomers of 1-phenylethanol
construction of a reporter strain by inserting a gene fusion of apcA, the first gene of the apc-bal operon, with the gene for the fluorescent protein mCherry into the chromosome. mCherry production is proportional to the applied acetophenone concentrations. The reporter strain allows quantification of acetophenone within a concentration range of 50 microM (detection limit) to 250 microM after 12 and 24 h. Production of the Apc-mCherry fusion protein in the reporter strain is highly specific and responds to acetophenone and both enantiomers of 1-phenylethanol
Muhr, E.; Leicht, O.; Sierra, S.; Thanbichler, M.; Heider, J.
A fluorescent bioreporter for acetophenone and 1-phenylethanol derived from a specifically induced catabolic operon
Front. Microbiol.
6
1561
2016
Aromatoleum aromaticum, Aromatoleum aromaticum (Q5P5G2 and Q5P5G3 and Q5P5G4 and Q5P5G5 and Q5P5G6), Aromatoleum aromaticum EbN1 (Q5P5G2 and Q5P5G3 and Q5P5G4 and Q5P5G5 and Q5P5G6)
Structure of the acetophenone carboxylase core complex prototype of a new class of ATP-dependent carboxylases/hydrolases
Sci. Rep.
7
39674
2017
Aromatoleum aromaticum, Aromatoleum aromaticum (Q5P5G2 and Q5P5G3 and Q5P5G4 and Q5P5G5 and Q5P5G6), Aromatoleum aromaticum EbN1 (Q5P5G2 and Q5P5G3 and Q5P5G4 and Q5P5G5 and Q5P5G6)
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6.4.1.8 acetophenone carboxylase
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