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Enzyme Nomenclature
Information on EC 6.4.1.8 - acetophenone carboxylase
for references in articles please use BRENDA:EC6.4.1.8
Word Map on EC 6.4.1.8
- 6.4.1.8
- aromaticum
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aromatoleum
- ethylbenzene
- acetone
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s-1-phenylethanol
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betaproteobacterium
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petroleum
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polyproline
- zn
-
bundle
- bicarbonate
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ii-like
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denitrifying
- bal
- atpase
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The expected taxonomic range for this enzyme is: Aromatoleum aromaticum
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EC NUMBER 
COMMENTARY 
6.4.1.8
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RECOMMENDED NAME
GeneOntology No.
acetophenone carboxylase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 ATP + acetophenone + HCO3- + H2O + H+ = 2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
acetophenone:carbon-dioxide ligase (ADP-forming)
The enzyme is involved in anaerobic degradation of ethylbenzene. No activity with acetone, butanone, 4-hydroxy-acetophenone or 4-amino-acetophenone.
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 ATP + 4-acetylpyridine + HCO3- + H2O + H+
2 ADP + 2 phosphate + 2-oxo-3-(pyridin-4-yl)propanoate
2 ATP + 4-acetylpyridine + HCO3- + H2O + H+
2 ADP + 2 phosphate + 2-oxo-3-(pyridin-4-yl)propanoate
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22% of the activity with acetophenone
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-
?
2 ATP + 4-acetylpyridine + HCO3- + H2O + H+
2 ADP + 2 phosphate + 2-oxo-3-(pyridin-4-yl)propanoate
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22% of the activity with acetophenone
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-
?
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
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-
-
-
?
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
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anaerobic ethylbenzene metabolism in the betaproteobacterium Aromatoleum aromaticum is initiated by anaerobic oxidation to acetophenone via (S)-1-phenylethanol. The subsequent carboxylation of acetophenone to benzoylacetate is catalyzed by an acetophenone-induced enzyme
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-
?
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
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the enzyme shows uncoupled ATPase activity with either bicarbonate or acetophenone in the absence of the second substrate. These observations indicate that both substrates may be phosphorylated. Purified enzyme shows no activity with GTP, CTP, UTP or ITP
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-
?
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
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-
-
-
?
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
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anaerobic ethylbenzene metabolism in the betaproteobacterium Aromatoleum aromaticum is initiated by anaerobic oxidation to acetophenone via (S)-1-phenylethanol. The subsequent carboxylation of acetophenone to benzoylacetate is catalyzed by an acetophenone-induced enzyme
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-
?
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
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the enzyme shows uncoupled ATPase activity with either bicarbonate or acetophenone in the absence of the second substrate. These observations indicate that both substrates may be phosphorylated. Purified enzyme shows no activity with GTP, CTP, UTP or ITP
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-
?
2 ATP + propiophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 4-oxo-4-phenylbutanoate
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108% of the activity with acetophenone
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-
?
2 ATP + propiophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 4-oxo-4-phenylbutanoate
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108% of the activity with acetophenone
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?
additional information
?
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no activity with acetone, butanone, 4-hydroxy-acetophenone or 4-amino-acetophenone
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-
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additional information
?
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no activity with acetone, butanone, 4-hydroxy-acetophenone or 4-amino-acetophenone
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
-
anaerobic ethylbenzene metabolism in the betaproteobacterium Aromatoleum aromaticum is initiated by anaerobic oxidation to acetophenone via (S)-1-phenylethanol. The subsequent carboxylation of acetophenone to benzoylacetate is catalyzed by an acetophenone-induced enzyme
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-
?
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
-
anaerobic ethylbenzene metabolism in the betaproteobacterium Aromatoleum aromaticum is initiated by anaerobic oxidation to acetophenone via (S)-1-phenylethanol. The subsequent carboxylation of acetophenone to benzoylacetate is catalyzed by an acetophenone-induced enzyme
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?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Zn2+
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the enzyme contains 1 Zn2+ ion per alphabetagammadelta core complex. In spite of the presence of Zn in the enzyme, it is strongly inhibited by Zn2+ ions
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
beta,gamma-Imido-ATP
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2 mM, complete inhibition of acetophenone carboxylase activity, non-competitive inhibition
Carbamoyl phosphate
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2 mM: 50% inhibition of ATPase activity. 8 mM: 50% inhibition of carboxylating activity
Ni2+
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1 mM: 50% inhibition of ATPase activity. 2.5 mM: 50% inhibition of carboxylation activity
Zn2+
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the enzyme contains 1 Zn2+ ion per alphabetagammadelta core complex. In spite of the presence of Zn in the enzyme, it is strongly inhibited by Zn2+ ions. 0.2 mM ZnCl2: 50% inhibition of ATPase activity and carboxylation activity
additional information
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acetophenone carboxylation activity in cell extracts is not sensitive to avidin, indicating that the enzyme is independent of biotin
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PDB
SCOP
CATH
ORGANISM
UNIPROT
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
15000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
34000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
70000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
75000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
87000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pentamer
pentamer
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
pentamer
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
during purification, acetophenone carboxylase dissociates into inactive subcomplexes consisting of the 70-, 15-, 87-, and 75-kDa subunits (apcABCD gene products) and the 34-kDa subunit (apcE gene product), respectively. Acetophenone carboxylase activity is restored by mixing the purified subcomplexes
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
heterologous expression of the apcEC-His6 subunit in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
acetophenone carboxylase is formed in ethylbenzene- and toluene-adapted cells
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acetophenone carboxylase is formed in ethylbenzene- and toluene-adapted cells; the enzyme is synthesized at high levels in cells grown on ethylbenzene or acetophenone, but not in cells grown on benzoate
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the enzyme is synthesized at high levels in cells grown on ethylbenzene or acetophenone, but not in cells grown on benzoate
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LINKS TO OTHER DATABASES (specific for EC-Number 6.4.1.8)
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