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Literature summary for 6.4.1.8 extracted from

  • Muhr, E.; Leicht, O.; Sierra, S.; Thanbichler, M.; Heider, J.
    A fluorescent bioreporter for acetophenone and 1-phenylethanol derived from a specifically induced catabolic operon (2016), Front. Microbiol., 6, 1561 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis construction of a reporter strain by inserting a gene fusion of apcA, the first gene of the apc-bal operon, with the gene for the fluorescent protein mCherry into the chromosome. mCherry production is proportional to the applied acetophenone concentrations. The reporter strain allows quantification of acetophenone within a concentration range of 50 microM (detection limit) to 250 microM after 12 and 24 h. Production of the Apc-mCherry fusion protein in the reporter strain is highly specific and responds to acetophenone and both enantiomers of 1-phenylethanol Aromatoleum aromaticum

Organism

Organism UniProt Comment Textmining
Aromatoleum aromaticum Q5P5G2 and Q5P5G3 and Q5P5G4 and Q5P5G5 and Q5P5G6 Q5P5G2 i.e. alpha-subunit, Q5P5G3 i.e. beta-subunit, Q5P5G4 i.e. gamma-subunit, Q5P5G5 i.e. delta-subunit, Q5P5G6 i.e. epsilon-subunit
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Aromatoleum aromaticum EbN1 Q5P5G2 and Q5P5G3 and Q5P5G4 and Q5P5G5 and Q5P5G6 Q5P5G2 i.e. alpha-subunit, Q5P5G3 i.e. beta-subunit, Q5P5G4 i.e. gamma-subunit, Q5P5G5 i.e. delta-subunit, Q5P5G6 i.e. epsilon-subunit
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Synonyms

Synonyms Comment Organism
Apc1 alpha-subunit Aromatoleum aromaticum
APC2 beta-subunit Aromatoleum aromaticum
Apc3 gamma-subunit Aromatoleum aromaticum
Apc4 delta-subunit Aromatoleum aromaticum
Apc5 epsilon-subunit Aromatoleum aromaticum

Expression

Organism Comment Expression
Aromatoleum aromaticum expression of alpha-subunit is proportional to the applied acetophenone concentrations up