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Information on EC 5.4.99.41 - lupeol synthase and Organism(s) Betula platyphylla and UniProt Accession Q8W3Z2

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.41 lupeol synthase
IUBMB Comments
Also forms some beta-amyrin. The recombinant enzyme from Arabidopsis thaliana gives a 1:1 mixture of lupeol and lupan-3beta,20-diol with small amounts of beta-amyrin, germanicol, taraxasterol and psi-taraxasterol. See EC 4.2.1.128 (lupan-3beta,20-diol synthase).
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This record set is specific for:
Betula platyphylla
UNIPROT: Q8W3Z2
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Word Map
The taxonomic range for the selected organisms is: Betula platyphylla
The enzyme appears in selected viruses and cellular organisms
Synonyms
lupeol synthase, rclus, mdosc5, atlup1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
lupeol synthase
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-
SYSTEMATIC NAME
IUBMB Comments
(3S)-2,3-epoxy-2,3-dihydrosqualene mutase (cyclizing, lupeol-forming)
Also forms some beta-amyrin. The recombinant enzyme from Arabidopsis thaliana [3] gives a 1:1 mixture of lupeol and lupan-3beta,20-diol with small amounts of beta-amyrin, germanicol, taraxasterol and psi-taraxasterol. See EC 4.2.1.128 (lupan-3beta,20-diol synthase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-oxidosqualene
lupeol
show the reaction diagram
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. japonica
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LUPS_BETPL
755
0
86473
Swiss-Prot
other Location (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
functional expression in Saccharomyces cerevisiae mutant lacking lanosterol synthase activity results in production of lupeol
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisae
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
LUS gene expression is found to increase abruptly 10 h after Phomopsis induction
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NaNO2 and L-arginine and NO donor sodium nitroprusside (SNP) are found to increase both NO production and LUS gene expression
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhang, H.; Shibuya, M.; Yokota, S.; Ebizuka, Y.
Oxidosqualene cyclases from cell suspension cultures of Betula platyphylla var. japonica: molecular evolution of oxidosqualene cyclases in higher plants
Biol. Pharm. Bull.
26
642-650
2003
Betula platyphylla (Q8W3Z2)
Manually annotated by BRENDA team
Fan, G.; Zhai, Q.; Zhan, Y.
Gene expression of lupeol synthase and biosynthesis of nitric oxide in cell suspension cultures of Betula platyphylla in response to a Phomopsis elicitor
Plant Mol. Biol. Rep.
31
296-302
2013
Betula platyphylla
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Manually annotated by BRENDA team