EC Number   |
|---|
   5.4.99.41 | - |
| 5.4.99.41 | expression in Sacchaormyces cerevisiae |
| 5.4.99.41 | expression in Saccharomyces cerevisae |
| 5.4.99.41 | expression in Saccharomyces cerevisiae |
| 5.4.99.41 | expression in Saccharomyces cerevisiae lacking lanosterol and ergosterol synthase activities |
| 5.4.99.41 | expression in Saccharomyces cerevisiae lacking lanosterol synthase activity |
| 5.4.99.41 | gene LUP1, recombinant expression in Rhodobacter capsulatus strain SB1003. and Synechocystis sp. PCC 6803 using promoters Pnif and PcoaT, coexpression with CAS1, THAS1, and MRN1. Subcloning in Saccharomyces cerevisiae strain GIL77. Isolation of triterpenes, while cycloartenol and lupeol represent typical plant triterpenes with tetracyclic plant sterol and pentacyclic plant triterpene scaffolds, respectively, thalianol and marneral exhibit more unusual tri- and monocyclic structures. Expression of LUP1 yields lupeol and a triterpenoid matching an oxidation product of lupeol, in both hosts. In Rhodobacter capsulatus and Synechocystis, LUP1-mediated formation of oxidized lupeol derivatives appears to be favored |
| 5.4.99.41 | gene LUS1, semi-quantitative RT-PCR enzyme expression analysis |
| 5.4.99.41 | gene OSC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana leaves via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of alpha-amyrin, beta-amyrin, and lupeol. Cells transiently expressing MdOSC5 cDNA are found to generate lupeol as a major component and beta-amyrin as a minor product, in a ratio of 95:5 |
