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Information on EC 5.4.99.12 - tRNA pseudouridine38-40 synthase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P31115

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.12 tRNA pseudouridine38-40 synthase
IUBMB Comments
The uridylate residues at positions 38, 39 and 40 of nearly all tRNAs are isomerized to pseudouridine. TruA specifically modifies uridines at positions 38, 39, and/or 40 in the anticodon stem loop of tRNAs with highly divergent sequences and structures .
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P31115
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
tRNA uridine38-40
=
tRNA pseudouridine38-40
Synonyms
trna pseudouridine synthase i, mpus3p, pus3p, pseudouridine synthase 3, deg1p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tRNA pseudouridine synthase 3
-
pseudouridine synthase I
-
-
SYSTEMATIC NAME
IUBMB Comments
tRNA-uridine38-40 uracil mutase
The uridylate residues at positions 38, 39 and 40 of nearly all tRNAs are isomerized to pseudouridine. TruA specifically modifies uridines at positions 38, 39, and/or 40 in the anticodon stem loop of tRNAs with highly divergent sequences and structures [1].
CAS REGISTRY NUMBER
COMMENTARY hide
430429-15-5
-
61506-89-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tRNA uridine38
tRNA pseudouridine38
show the reaction diagram
tRNA uridine39
tRNA pseudouridine39
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tRNA uridine38
tRNA pseudouridine38
show the reaction diagram
tRNA uridine39
tRNA pseudouridine39
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-fluorouracil tRNAPhe
-
enzyme forms a covalent adduct with 5-fluorouracil-tRNA to form a putative analog of a steady-state intermediate in the normal reaction pathway. The putative Asp nucleophile is attached to the 6-position of the target 5-fluorouracil-tRNA to form a stable covalent adduct, which can undergo O-acyl hydrolytic cleavage, and the intermediate contains an intact N-glycosidic bond linking the modified base to the polynucleotide chain
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
activity of yeast extract and purified yeast enzyme
37
activity of Escherichia coli extract
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D151A
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lecointe, F.; Simos, G.; Sauer, A.; Hurt, E.; Motorin, Y.; Grosjean, H.
Characterization of yeast protein Deg1 as pseudouridine synthase (pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop
J. Biol. Chem.
273
1316-1323
1998
Saccharomyces cerevisiae (P31115), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Lecointe, F.; Namy, O.; Hatin, I.; Simos, G.; Rousset, J-P.; Grosjean, H.
Lack of pseudouridine 38/39 in the anticodon arm of yeast cytoplasmic tRNA decreases in vivo recoding efficiency
J. Biol. Chem.
277
30445-30453
2002
Saccharomyces cerevisiae (P31115), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Gu, X.; Liu, Y.; Santi, D.V.
The mechanism of pseudouridine synthase I as deduced from its interaction with 5-fluorouracil-tRNA
Proc. Natl. Acad. Sci. USA
96
14270-14275
1999
Saccharomyces cerevisiae, Escherichia coli (P07649)
Manually annotated by BRENDA team
Hepfer, C.E.; Arnold-Croop, S.; Fogell, H.; Steudel, K.G.; Moon, M.; Roff, A.; Zaikoski, S.; Rickman, A.; Komsisky, K.; Harbaugh, D.L.; Lang, G.I.; Keil, R.L.
DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae
Mol. Genet. Genomics
274
528-538
2005
Saccharomyces cerevisiae (P31115), Saccharomyces cerevisiae
Manually annotated by BRENDA team