EC Number   |
General Information |
Reference |
|---|
   5.4.99.12 | malfunction |
deletion of the PUS3 gene has an effect on the efficiency of the translation process. Reduced readthrough efficiency of each stop codon by natural nonsense suppressor tRNAs |
648865 |
| 5.4.99.12 | malfunction |
disruption of the DEG1 gene is not lethal but reduces considerably the yeast growth rate, especially at an elevated temperature, 37 °C |
648860 |
| 5.4.99.12 | malfunction |
hisT mutants of Salmonella typhimurium lack the enzyme that modifies uridine to pseudouridine in the anticodon regions of many tRNAs. The regulation of a large number of amino acid biosynthetic pathways is altered by the hisT mutation |
704370 |
| 5.4.99.12 | malfunction |
tRNA pseudouridine synthase is able to complement the type III gene expression defect of the fimV mutant. Thus fimV and truA form an operon and fimV mutation has a polar effect on truA. A truA mutant is defective in type III gene expression while its twitching motility is unaffected, and a truA clone is able to complement the type III secretion defect |
705552 |
| 5.4.99.12 | physiological function |
deletion of the PUS3 gene, encoding the enzyme that introduces pseudouridines at position 38 or 39 in tRNA, has an effect on the efficiency of the translation process. In the mutant, there is a reduced readthrough efficiency of each stop codon by natural nonsense suppressor tRNAs. This effect is solely due to the absence of pseudouridine 38 or 39 in tRNA. The presence of pseudouridine 38 or 39 in the tRNA anticodon arm enhances misreading of certain codons by natural nonsense tRNAs as well as promotes frameshifting on slippery sequences in yeast |
648865 |
| 5.4.99.12 | physiological function |
Pus3p is unique in its ability to modulate frameshifting and readthrough events during translation. This aspect of its activity may be responsible for HOT1 recombination phenotypes observed in deg1 mutants |
713040 |
| 5.4.99.12 | physiological function |
the truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes |
705552 |
| 5.4.99.12 | physiological function |
TruA utilizes the intrinsic flexibility of the anticodon stem loop for site promiscuity and also to select against intrinsically stable tRNAs to avoid their overstabilization through pseudouridylation, thereby maintaining the balance between the flexibility and stability required for its biological function |
681882 |
| 5.4.99.12 | physiological function |
TruA utilizes the intrinsic flexibility of the ASL for site promiscuity and also to select against intrinsically stable tRNAs to avoid their overstabilization through pseudouridylation, thereby maintaining the balance between the flexibility and stability required for its biological function |
681882 |
