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Information on EC 5.1.3.2 - UDP-glucose 4-epimerase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9C7W7

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EC Tree
     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.2 UDP-glucose 4-epimerase
IUBMB Comments
Requires NAD+. Also acts on UDP-2-deoxyglucose.
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Select one or more organisms in this record:
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9C7W7
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The taxonomic range for the selected organisms is: Arabidopsis thaliana
Synonyms
4-Epimerase, 6xHis-rGalE, An14g03820, AtUGE1, AtUGE2, AtUGE3, AtUGE4, AtUGE5, CaGAL10, CapD, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4-Epimerase
-
-
-
-
AtUGE1
289312
-
AtUGE2
289313
-
AtUGE3
289314
-
AtUGE4
289315
-
AtUGE5
289316
-
Epimerase, uridine diphosphoglucose
-
-
-
-
Galactowaldenase
-
-
-
-
UDP-D-galactose 4-epimerase
-
-
-
-
UDP-D-glucose/UDP-D-galactose 4-epimerase
302
-
UDP-galactose 4-epimerase
-
-
-
-
UDP-Glc 4-epimerase
289312, 289313, 289314, 289315, 289316
-
UDP-glucose 4-epimerase
UDP-glucose 4-epimerase 4
289315
-
UDP-glucose epimerase
-
-
-
-
UDPG-4-epimerase
-
-
-
-
UDPgalactose 4-epimerase
-
-
-
-
UGE1:GUS
302
-
UGE3:GUS
302
-
Uridine diphosphate galactose 4-epimerase
-
-
-
-
Uridine diphosphate glucose 4-epimerase
-
-
-
-
Uridine diphospho-galactose-4-epimerase
-
-
-
-
Uridine diphosphoglucose 4-epimerase
-
-
-
-
Uridine diphosphoglucose epimerase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
epimerization
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose 4-epimerase
Requires NAD+. Also acts on UDP-2-deoxyglucose.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-89-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-glucose
UDP-D-galactose
show the reaction diagram
-
-
-
r
UDP-alpha-D-glucose
UDP-alpha-D-galactose
show the reaction diagram
-
-
-
-
r
UDP-D-glucose
UDP-D-galactose
show the reaction diagram
UDP-galactose
UDP-glucose
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-D-glucose
UDP-D-galactose
show the reaction diagram
Q42605, Q8LDN8, Q9C7W7, Q9SN58, Q9T0A7
-
-
-
r
UDP-alpha-D-glucose
UDP-alpha-D-galactose
show the reaction diagram
-
-
-
-
r
UDP-D-glucose
UDP-D-galactose
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
-
increases the activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
slightly inhibiting
NADPH
-
very weak inhibitory effect
Salt
-
moderately inhibited by high salt concentrations
additional information
-
inhibited by combination of 100 microM NADH and 10 microM NAD+
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.057
UDP-D-galactose
recombinant enzyme UGE4, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase
0.068 - 0.15
UDP-D-galactose
0.1 - 0.3
UDP-galactose
0.09 - 0.76
UDP-glucose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20
UDP-D-galactose
recombinant enzyme UGE4
9 - 64
UDP-D-galactose
23 - 128
UDP-galactose
2 - 33
UDP-glucose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
350
UDP-D-galactose
recombinant enzyme UGE4, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase
103 - 578
UDP-D-galactose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7
-
MES buffer
6 - 10.5
-
about 65% of maximal activity at pH 6 and 10.5
6.5 - 9.5
-
Tris/HCl buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
the gene is expressed in all tissues, with highest expression level in the stems and roots
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
identification of a mutant allele of UDP-glucose epimerase 4 (UGE4)/root hair defective 1/root epidermal bulgar 1, which is a mutant with swollen root epidermal cells and has an altered sugar composition in cell wall polysaccharides. Importantly, these defects including aggregate formation are restored by supplementation of D-galactose in the medium. Intracellular aggregates in the uge4 mutant contains endomembrane markers in the secretory and vacuolar pathways. The lack of UGE4 function affects the structure of the endoplasmic reticulum or trafficking of the endoplasmic reticulum marker. Phenotype, overview. Disruption of the cytoskeleton is not a primary cause of the endomembrane aggregates in the uge4 mutant
physiological function
physiological function
bifunctional cytosolic UDP-glucose 4-epimerase catalyses the interconversion between UDP-D-xylose and UDP-L-arabinose in plants
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
UGE4_ARATH
348
0
38123
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
gel filtration
40200
-
gel filtration
40300
-
gel filtration
40800
-
gel filtration
41100
-
gel filtration
42000
-
nickel affinity chromatography
84000
-
Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model.
91000
-
Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model.
98000
-
Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model.
103000
-
Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
identification of a mutant allele of UDP-glucose epimerase 4 (UGE4)/root hair defective 1/root epidermal bulgar 1, which is a mutant with swollen root epidermal cells and has an altered sugar composition in cell wall polysaccharides, phenotype, overview
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
purification of the recombinant enzyme by Ni-NTA affinity chromatography
purification of the recombinant Arabidopsis enzyme by Ni-NTA affinity chromatography
purification of the recombinant enzyme by Ni-NTA affinity chromatography
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expression in Agrobacterium tumefaciens
recombinant protein is expressed in Escherichia coli with C-terminal His-tag
expression in Agrobacterium tumefaciens
expression in Escherichia coli
-
expression in Escherichia coli and Saccharomyces cerevisiae
-
recombinant protein is expressed in Escherichia coli with C-terminal His-tag
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dörmann, P.; Benning, C.
Functional expression of uridine 5'-diphospho-glucose 4-epimerase (EC 5.1.3.2) from Arabidopsis thaliana in Saccharomyces cerevisiae and Escherichia coli
Arch. Biochem. Biophys.
327
27-34
1996
Arabidopsis thaliana
Manually annotated by BRENDA team
Wubben, M.J.2nd.; Rodermel, S.R.; Baum, T.J.
Mutation of a UDP-glucose-4-epimerase alters nematode susceptibility and ethylene responses in Arabidopsis roots
Plant J.
40
712-724
2004
Arabidopsis thaliana
Manually annotated by BRENDA team
Barber, C.; Roesti, J.; Rawat, A.; Findlay, K.; Roberts, K.; Seifert, G.J.
Distinct properties of the five UDP-D-glucose/UDP-D-galactose 4-epimerase isoforms of Arabidopsis thaliana
J. Biol. Chem.
281
17276-17285
2006
Arabidopsis thaliana
Manually annotated by BRENDA team
Roesti, J.; Barton, C.J.; Albrecht, S.; Dupree, P.; Pauly, M.; Findlay, K.; Roberts, K.; Seifert, G.J.
UDP-glucose 4-epimerase isoforms UGE2 and UGE4 cooperate in providing UDP-galactose for cell wall biosynthesis and growth of Arabidopsis thaliana
Plant Cell
19
1565-1579
2007
Arabidopsis thaliana
Manually annotated by BRENDA team
Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.
Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots
New Phytol.
184
457-472
2009
Arabidopsis thaliana (Q42605), Arabidopsis thaliana (Q8LDN8), Arabidopsis thaliana (Q9C7W7), Arabidopsis thaliana (Q9SN58), Arabidopsis thaliana (Q9T0A7)
Manually annotated by BRENDA team
Kotake, T.; Takata, R.; Verma, R.; Takaba, M.; Yamaguchi, D.; Orita, T.; Kaneko, S.; Matsuoka, K.; Koyama, T.; Reiter, W.D.; Tsumuraya, Y.
Bifunctional cytosolic UDP-glucose 4-epimerases catalyse the interconversion between UDP-D-xylose and UDP-L-arabinose in plants
Biochem. J.
424
169-177
2009
Arabidopsis thaliana (Q42605), Arabidopsis thaliana (Q8LDN8), Arabidopsis thaliana (Q9C7W7), Arabidopsis thaliana (Q9SN58), Arabidopsis thaliana (Q9T0A7), Pisum sativum (B0M3E8), Pisum sativum
Manually annotated by BRENDA team
Wang, S.; Ito, T.; Uehara, M.; Naito, S.; Takano, J.
UDP-D-galactose synthesis by UDP-glucose 4-epimerase 4 is required for organization of the trans-Golgi network/early endosome in Arabidopsis thaliana root epidermal cells
J. Plant Res.
128
863-873
2015
Arabidopsis thaliana, Arabidopsis thaliana (Q9C7W7), Arabidopsis thaliana Col-0 (Q9C7W7)
Manually annotated by BRENDA team
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