EC Number |
---|
5.1.3.2 | - |
5.1.3.2 | DNA and amino acid sequence determination and analysis, quantitative real-time reverse transcription PCR enzyme expression analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
5.1.3.2 | expressed in Escherichia coli BL21(DE3) cells |
5.1.3.2 | expressed in Escherichia coli ER2566 cells |
5.1.3.2 | expressed in Escherichia coli Rosetta cells and in a gal10-null strain of Saccharomyces cerevisiae strain JFy3835 |
5.1.3.2 | expression in Agrobacterium tumefaciens |
5.1.3.2 | expression in Escherichia coli |
5.1.3.2 | expression in Escherichia coli and Saccharomyces cerevisiae |
5.1.3.2 | expression in GALE-null line of Chineses hamster ovary cells. GALE-null cells accumulats abnormally high levels of Hal-1-P and UDP-Gal and abnormally low levels of UDP-Glc and UDP-GLcNAc in the presence of galactose. Human GALE expression corrects each of theses defects |
5.1.3.2 | expression in GALE-null line of Chinesese hamster ovary cells. Enzyme from Escherichia coli can not restore the metabolic balance, because unlike the mammalian enzyme it can not catalyze the interconversion of UDP-GalNAc and UDP-GlcNAc |