EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    5.1.3.2 | 0.0000097 |
- |
D-galactose |
in McIlvaine buffer (pH 7.5), at 35°C |
713793 |
| 5.1.3.2 | 0.000023 |
- |
D-tagatose |
in McIlvaine buffer (pH 7.5), at 35°C |
713793 |
| 5.1.3.2 | 0.49 |
- |
UDP-alpha-D-glucose |
pH 6.5, 60°C |
721850 |
| 5.1.3.2 | 0.78 |
- |
UDP-galactose |
recombinant enzyme, 100 mM glycine-NaOH (pH 8.9), 4 mM UDP-galactose, 1 mM beta-NAD+, 8.3 mM MgCl2, and 5.4 U of UDP-glucose dehydrogenase |
702074 |
| 5.1.3.2 | 0.79 |
- |
UDP-galactose |
mutant enzyme K153N, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C |
714602 |
| 5.1.3.2 | 1.55 |
- |
UDP-N-acetyl-alpha-D-glucosamine |
pH 7.5, 45°C, mutant G118A/G119A |
726782 |
| 5.1.3.2 | 2.9 |
- |
UDP-alpha-D-glucose |
pH 6.2, temperature not specified in the publication, recombinant mutant Y149F |
726547 |
| 5.1.3.2 | 3.13 |
- |
UDP-N-acetyl-alpha-D-glucosamine |
pH 7.5, 45°C, mutant S279Y |
726782 |
| 5.1.3.2 | 4.34 |
- |
UDP-alpha-D-galactose |
pH 6.5, 60°C |
721850 |
| 5.1.3.2 | 5.39 |
- |
UDP-N-acetyl-alpha-D-glucosamine |
pH 7.5, 45°C, mutant S116A |
726782 |
