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Information on EC 5.1.3.1 - ribulose-phosphate 3-epimerase and Organism(s) Spinacia oleracea and UniProt Accession Q43157

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EC Tree
     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.1 ribulose-phosphate 3-epimerase
IUBMB Comments
The enzyme also converts D-erythrose 4-phosphate into D-erythrulose 4-phosphate and D-threose 4-phosphate.
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Select one or more organisms in this record:
This record set is specific for:
Spinacia oleracea
UNIPROT: Q43157
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The taxonomic range for the selected organisms is: Spinacia oleracea
Synonyms
cyt-RPEase, D-ribulose 5-phosphate 3-epimerase, D-Ribulose phosphate-3-epimerase, D-Ribulose-5-P 3-epimerase, D-ribulose-5-phosphate 3-epimerase, D-Ribulose-5-phosphate epimerase, D-ribulose-5-phosphate-3-epimerase, D-Xylulose-5-phosphate 3-epimerase, Epimerase, ribulose phosphate 3-, Pentose-5-phosphate 3-epimerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-Ribulose phosphate-3-epimerase
-
-
-
-
D-Ribulose-5-P 3-epimerase
-
-
-
-
D-Ribulose-5-phosphate epimerase
-
-
-
-
D-Xylulose-5-phosphate 3-epimerase
-
-
-
-
Epimerase, ribulose phosphate 3-
-
-
-
-
Pentose-5-phosphate 3-epimerase
-
-
-
-
Phosphoketopentose 3-epimerase
-
-
-
-
Phosphoketopentose epimerase
-
-
-
-
Phosphoribulose epimerase
-
-
-
-
PPE
-
-
-
-
R5P3E
-
-
-
-
Ribulose 5-phosphate 3-epimerase
-
-
-
-
Ribulose phosphate 3-epimerase
-
-
-
-
RPE
-
-
-
-
Ru5P epimerase
Xylulose phosphate 3-epimerase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
epimerization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-ribulose-5-phosphate 3-epimerase
The enzyme also converts D-erythrose 4-phosphate into D-erythrulose 4-phosphate and D-threose 4-phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-20-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-Ribulose 5-phosphate
D-Xylulose 5-phosphate
show the reaction diagram
-
-
?
D-Ribulose 5-phosphate
D-Xylulose 5-phosphate
show the reaction diagram
additional information
?
-
-
enzyme is integral to both the Calvin cycle and the oxidative pentose phosphate pathway
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-
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
enzyme is integral to both the Calvin cycle and the oxidative pentose phosphate pathway
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no requirement for a divalent metal ion in catalysis
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DL-alpha-glycerophosphate
-
competitive
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.22 - 2.1
D-ribulose 5-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00033 - 7100
D-ribulose 5-phosphate
0.138
D-ribulose 5-phosphate
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
DL-alpha-glycerophosphate
-
25°C, pH 8.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.3
-
reaction with D-ribulose 5-phosphate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
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pH 6.5: about 90% of maximal activity, pH 9.0: 95% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 6.8
-
wild-type enzyme and mutant enzyme D186E, denaturing isoelectric focusing, pH gradient 5-8
6.6
-
mutant enzymes D186A and D186N, denaturing isoelectric focusing, pH gradient 5-8
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
stroma; stroma, 90% of the total epimerase is associated with thylakoid membranes
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
RPE_SPIOL
285
0
30366
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23000
-
8 * 23000, SDS-PAGE
25000
-
8 * 25000, SDS-PAGE
180000
-
gel filtration
200000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D186A
-
KM-value for D-ribulose 5-phosphate is increased 10fold, turnover-number is decreased to less than 0.1% of the wild-type value
D186E
-
KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value
D186N
-
KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
DL-alpha-glycerophosphate or ethanol stabilize the extremely labile recombinant enzyme, but are unable to reverse the spontaneous loss of activity
-
drastically destabilized by 2-mercaptoethanol
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DTT destabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
2°C, wether in crude extracts or highly purified, the recombinant enzyme is inherently unstable
-
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
mutant enzymes D186A, D186N, D186E
-
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nowitzki, U.; Wyrich, R.; Westhoff, P.; Henze, K.; Scharrenberger, C.; Martin, W.
Cloning of the amphibolic Calvin cycle/OPPP enzyme D-ribulose-5-phosphate 3-epimerase (EC 5.1.3.1) from spinach chloroplasts: functional and evolutionary aspects
Plant Mol. Biol.
29
1279-1291
1995
Spinacia oleracea
Manually annotated by BRENDA team
Teige, M.; Melzer, M.; Suss, K.H.
Purification, properties and in situ localization of the amphibolic enzymes D-ribulose 5-phosphate 3-epimerase and transketolase from spinach chloroplasts
Eur. J. Biochem.
252
237-244
1989
Spinacia oleracea
Manually annotated by BRENDA team
Chen, Y.R.; Larimer, F.W.; Serpersu, E.H.; Hartman, F.C.
Identification of a catalytic aspartyl residue of D-ribulose 5-phosphate 3-epimerase by site-directed mutagenesis
J. Biol. Chem.
274
2132-2136
1999
Spinacia oleracea (Q43157), Spinacia oleracea
Manually annotated by BRENDA team
Chen, Y.R.; Hartman, F.C.; Lu, T.Y.; Larimer, F.W.
D-Ribulose-5-phosphate 3-epimerase: cloning and heterologous expression of the spinach gene, and purification and characterization of the recombinant enzyme
Plant Physiol.
118
199-207
1998
Spinacia oleracea (Q43157), Spinacia oleracea
Manually annotated by BRENDA team
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