EC Number |
Protein Variants |
Reference |
---|
5.1.3.1 | D175A |
inactive |
714945 |
5.1.3.1 | D176A |
no detectable activity, substrate= 10 mM D-ribulose 5-phosphate |
672127 |
5.1.3.1 | D186A |
KM-value for D-ribulose 5-phosphate is increased 10fold, turnover-number is decreased to less than 0.1% of the wild-type value |
651933 |
5.1.3.1 | D186E |
KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value |
651933 |
5.1.3.1 | D186N |
KM-value for D-ribulose 5-phosphate is unaltered, turnover-number is decreased to less than 0.1% of the wild-type value |
651933 |
5.1.3.1 | D36A |
no detectable activity, substrate= 10 mM D-ribulose 5-phosphate |
672127 |
5.1.3.1 | D37A |
the mutation almost abolishes the enzymatic activity |
714945 |
5.1.3.1 | H34A |
mutant discloses decreased affinity for Zn2+, kcat = 200/sec, substrate = 10 mM D-ribulose 5-phosphate, +10 micromol/l ZnCl2 |
672127 |
5.1.3.1 | H35A |
the mutation almost abolishes the enzymatic activity |
714945 |
5.1.3.1 | H67A |
mutant discloses decreased affinity for Zn2+, kcat = 270/sec, substrate = 10 mM D-ribulose 5-phosphate, +10 micromol/l ZnCl2 |
672127 |