We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
IUBMB Comments Isolated from the root of the plant Thapsia garganica . The enzyme also produces germacrene D, bicyclogermacrene and traces of other sesquiterpenoids. See EC 4.2.3.77 , (+)-germacrene D synthase and EC 4.2.3.100 , bicyclogermacrene synthase.
Synonyms
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TgTPS2
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2E,6E)-farnesyl diphosphate + H2O = kunzeaol + diphosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (kunzeaol forming)
Isolated from the root of the plant Thapsia garganica. The enzyme also produces germacrene D, bicyclogermacrene and traces of other sesquiterpenoids. See EC 4.2.3.77, (+)-germacrene D synthase and EC 4.2.3.100, bicyclogermacrene synthase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2E,6E)-farnesyl diphosphate + H2O
epikunzeaol + diphosphate
-
Substrates: -
?
(2E,6E)-farnesyl diphosphate + H2O
kunzeaol + diphosphate
additional information
?
-
Substrates: radioactive assay method
?
(2E,6E)-farnesyl diphosphate + H2O
kunzeaol + diphosphate
-
Substrates: -
?
(2E,6E)-farnesyl diphosphate + H2O
kunzeaol + diphosphate
Substrates: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2E,6E)-farnesyl diphosphate + H2O
kunzeaol + diphosphate
Substrates: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EDTA
-
100 mM, complete loss of activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00055 - 0.0026
(2E,6E)-farnesyl diphosphate
additional information
additional information
Michaelis-Menten kinetics
-
0.00055
(2E,6E)-farnesyl diphosphate
pH 7.5, temperature not specified in the publication
0.0026
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 28°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.03 - 0.29
(2E,6E)-farnesyl diphosphate
0.03
(2E,6E)-farnesyl diphosphate
-
pH 7.5, 28°C
0.29
(2E,6E)-farnesyl diphosphate
pH 7.5, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
527.3
(2E,6E)-farnesyl diphosphate
pH 7.5, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
enzyme is involved in thapsigargin biosynthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STS2_THAGA
561
0
64663
Swiss-Prot
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
64700
-
x * 64700, calculated, x * 65000, SDS-PAGE
65000
-
x * 64700, calculated, x * 65000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 64700, calculated, x * 65000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
recombinant His6-tagged enzyme from Saccharomyces cerevisiae strain BY4742 by affinity chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Nicotiana benthamiana
-
expression in Saccharomyces cerevisiae and Escherichia coli
-
gene STS2, recombinant overexpression of His6-tagged enzyme in Saccharomyces cerevisiae strain BY4742 and of (YFP)2-tagged enzyme in Saccharomyces cerevisiae
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Pickel, B.; Drew, D.P.; Manczak, T.; Weitzel, C.; Simonsen, H.T.; Ro, D.K.
Identification and characterization of a kunzeaol synthase from Thapsia garganica: implications for the biosynthesis of the pharmaceutical thapsigargin
Biochem. J.
448
261-271
2012
Elaeoselinum garganicum
brenda
Manczak, T.; Simonsen, H.T.
Insight into biochemical characterization of plant sesquiterpene synthases
Anal. Chem. Insights
11
1-7
2016
Elaeoselinum garganicum (K4LMW2)
brenda
Andersen, T.B.; Rasmussen, S.A.; Christensen, S.B.; Simonsen, H.T.
Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy
Phytochemistry
157
168-174
2019
Thapsia laciniata
brenda
html completed
show
top
