Literature summary for 4.2.3.143 extracted from

Pickel, B.; Drew, D.P.; Manczak, T.; Weitzel, C.; Simonsen, H.T.; Ro, D.K.
Identification and characterization of a kunzeaol synthase from Thapsia garganica: implications for the biosynthesis of the pharmaceutical thapsigargin (2012), Biochem. J., 448, 261-271.

Search for more literature for 4.2.3.143

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Saccharomyces cerevisiae and Escherichia coli	Thapsia garganica

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	100 mM, complete loss of activity	Thapsia garganica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0026	-	(2E,6E)-farnesyl diphosphate	pH 7.5, 28°C	Thapsia garganica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64700	-	x * 64700, calculated, x * 65000, SDS-PAGE	Thapsia garganica
65000	-	x * 64700, calculated, x * 65000, SDS-PAGE	Thapsia garganica

Organism

Organism	UniProt	Comment	Textmining
Thapsia garganica	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	-	Thapsia garganica	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + H2O	-	Thapsia garganica	kunzeaol + diphosphate	i.e. 6beta-hydroxygermacra-1(10),4-diene, (1R,2E,6E,10R)-3,7-dimethyl-10-isopropylcyclodeca-2,6-dienol, major product, plus germacrene D, bicyclogermacrene and two more minor products	?

Subunits

Subunits	Comment	Organism
?	x * 64700, calculated, x * 65000, SDS-PAGE	Thapsia garganica

Synonyms

Synonyms	Comment	Organism
TPS2	-	Thapsia garganica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.03	-	(2E,6E)-farnesyl diphosphate	pH 7.5, 28°C	Thapsia garganica

General Information

General Information	Comment	Organism
physiological function	enzyme is involved in thapsigargin biosynthesis	Thapsia garganica

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Release 2023.1 (January 2023)