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Literature summary for 4.2.3.143 extracted from
- Insight into biochemical characterization of plant sesquiterpene synthases (2016), Anal. Chem. Insights, 11, 1-7 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene STS2, recombinant overexpression of His6-tagged enzyme in Saccharomyces cerevisiae strain BY4742 and of (YFP)2-tagged enzyme in Saccharomyces cerevisiae | Thapsia garganica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Thapsia garganica |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Thapsia garganica | |
| 0.00055 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, temperature not specified in the publication | Thapsia garganica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Thapsia garganica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | Thapsia garganica | - |
kunzeaol + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thapsia garganica | K4LMW2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Saccharomyces cerevisiae strain BY4742 by affinity chromatography | Thapsia garganica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | - |
Thapsia garganica | kunzeaol + diphosphate | - |
? | |
| additional information | radioactive assay method | Thapsia garganica | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| STS2 | - |
Thapsia garganica |
| TgTPS2 | - |
Thapsia garganica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, temperature not specified in the publication | Thapsia garganica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thapsia garganica |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 527.3 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, temperature not specified in the publication | Thapsia garganica | |
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