Cloned (Comment) | Organism |
---|---|
gene STS2, recombinant overexpression of His6-tagged enzyme in Saccharomyces cerevisiae strain BY4742 and of (YFP)2-tagged enzyme in Saccharomyces cerevisiae | Thapsia garganica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Thapsia garganica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Thapsia garganica | |
0.00055 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, temperature not specified in the publication | Thapsia garganica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thapsia garganica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | Thapsia garganica | - |
kunzeaol + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thapsia garganica | K4LMW2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Saccharomyces cerevisiae strain BY4742 by affinity chromatography | Thapsia garganica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | - |
Thapsia garganica | kunzeaol + diphosphate | - |
? | |
additional information | radioactive assay method | Thapsia garganica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
STS2 | - |
Thapsia garganica |
TgTPS2 | - |
Thapsia garganica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, temperature not specified in the publication | Thapsia garganica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thapsia garganica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
527.3 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, temperature not specified in the publication | Thapsia garganica |