Any feedback?
Information on EC 4.2.3.104 - alpha-humulene synthase
for references in articles please use BRENDA:EC4.2.3.104Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
4.2.3.104 alpha-humulene synthaseIUBMB Comments
The enzyme from Zingiber zerumbet, shampoo ginger, also gives traces of beta-caryophyllene.
Specify your search results
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
alpha-humulene synthase, sesquiterpene synthase1, (e)-beta-caryophyllene/alpha-humulene synthase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
beta-caryophyllene/alpha-humulene synthase
CAHS
TPS1
ZSS1
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = alpha-humulene + diphosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (alpha-humulene-forming)
The enzyme from Zingiber zerumbet, shampoo ginger, also gives traces of beta-caryophyllene.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2E)-geranyl diphosphate + H2O
geraniol + diphosphate
the enzyme produces limonene, linalool, alpha-terpineol, and geraniol from geranyl diphosphate. Geraniol is the main product
-
-
?
(2Z)-neryl diphosphate
limonene + diphosphate
the enzyme produces linalool, alpha-terpineol, geraniol, alpha-terpinolene, p-menth-2-en-1-ol, piperitol, and nerol from neryl diphosphate. Limonene is the main product
-
-
?
2 (2E,6E)-farnesyl diphosphate
beta-caryophyllene + alpha-humulene + 2 diphosphate
-
-
-
?
4 (2E,6E)-farnesyl diphosphate
guaia-1(10),11-diene + guaia-1(5),11-diene + alpha-humulene + beta-elemene + 4 diphosphate
-
products are 55% guaia-1(10),11-diene, 14% guaia-1(5),11-diene, 10% alpha-humulene, 10% beta-elemene, 6% alloaromadendrene and 5% beta-caryophyllene
-
?
(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
-
enzyme produces beta-caryophyllene and alpha-humulene in a ratio of 71:29
-
?
(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
-
the main product is alpha-humulene, second product is beta-caryophyllene
-
?
(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
-
-
95% yield of alpha-humulene besides the minor product beta-caryophyllene (5%)
-
?
additional information
?
-
the recombinant alpha-humulene synthase isozyme HS1 produces 94.6% alpha-humulene and 5.4% beta-caryophyllene
-
-
?
additional information
?
-
the recombinant alpha-humulene synthase isozyme HS1 produces 94.6% alpha-humulene and 5.4% beta-caryophyllene
-
-
?
additional information
?
-
the recombinant alpha-humulene synthase isozyme HS1 produces 94.6% alpha-humulene and 5.4% beta-caryophyllene
-
-
?
additional information
?
-
the recombinant alpha-humulene synthase isozyme HS2 produces 95.0% alpha-humulene and 5.0% beta-caryophyllene
-
-
?
additional information
?
-
the recombinant alpha-humulene synthase isozyme HS2 produces 95.0% alpha-humulene and 5.0% beta-caryophyllene
-
-
?
additional information
?
-
the recombinant alpha-humulene synthase isozyme HS2 produces 95.0% alpha-humulene and 5.0% beta-caryophyllene
-
-
?
additional information
?
-
the recombinant alpha-humulene synthase isozyme HS3 produces 93.5% alpha-humulene and 6.5% beta-caryophyllene
-
-
?
additional information
?
-
the recombinant alpha-humulene synthase isozyme HS3 produces 93.5% alpha-humulene and 6.5% beta-caryophyllene
-
-
?
additional information
?
-
the recombinant alpha-humulene synthase isozyme HS3 produces 93.5% alpha-humulene and 6.5% beta-caryophyllene
-
-
?
additional information
?
-
malachite green assay adapted to rapidly assay terpene synthase activity for activity determination, and GS-MS analysis
-
-
?
additional information
?
-
the recombinant alpha-humulene synthase produces 93.45% alpha-humulene and 5.5% beta-caryophyllene. Product determination by gas chromatography-flame ionization detection (GC-FID)
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
alpha-humulene + diphosphate
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0736
(2E)-geranyl diphosphate
pH 7.5, 38°C, recombinant His-tagged enzyme
0.068
(2Z)-neryl diphosphate
pH 7.5, 38°C, recombinant His-tagged enzyme
0.0289
(2E,6E)-farnesyl diphosphate
30°C, pH not specified in the publication
0.0303
(2E,6E)-farnesyl diphosphate
pH 7.5, 38°C, recombinant His-tagged enzyme
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.8
(2E)-geranyl diphosphate
pH 7.5, 38°C, recombinant His-tagged enzyme
4.26
(2Z)-neryl diphosphate
pH 7.5, 38°C, recombinant His-tagged enzyme
5.61
(2E,6E)-farnesyl diphosphate
pH 7.5, 38°C, recombinant His-tagged enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.45
substrate (2E)-geranyl diphosphate, pH 7.5, 38°C, recombinant His-tagged enzyme
3.81
substrate (2Z)-neryl diphosphate, pH 7.5, 38°C, recombinant His-tagged enzyme
5.08
substrate (2E,6E)-farnesyl diphosphate, pH 7.5, 38°C, recombinant His-tagged enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 9
activity range, profile overview, recombinant His-tagged enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
38
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
32 - 44
activity range, profile overview, recombinant His-tagged enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
expression is hardly detectable in other organs than leaf and pericarp
brenda
additional information
expression is hardly detectable in other organs than leaf and pericarp
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
additional information
the enzyme has no N-terminal transit peptide
-
brenda
additional information
the enzyme has no N-terminal transit peptide
-
brenda
additional information
the enzyme has no N-terminal transit peptide
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
physiological function
additional information
metabolism
-
alpha-humulene synthase is a key enzyme of zerumbone biosynthesis
metabolism
the enzyme is involved in the biosynthetic pathways for sesquiterpenes found in agarwood and cell suspension cultures, putative pathway, overview. Various post-transcriptional, translational, or post-translational regulatory mechanisms affecting the expression or activity of alpha-humulene and delta-guaiene synthases are possible
metabolism
the enzyme is involved in the zerumbone biosynthesis in Zingiber zerumbet, proposed pathway and mechanism, overview
physiological function
after tobacco rattlevirus-based virus induced gene silencing, beta-elemene, guaia-1(5),11-diene and guaia-1(10),11-diene are undetectable in suppressed leaves
physiological function
alpha-humulene is a common sesquiterpene widely distributed in plants, the synthase from Zingiber zerumbet is the only known sesquiterpene synthase to date catalyzing the formation of alpha-humulene as major product
additional information
sesquiterpene synthase alpha-humulene synthase (HUM) from Zingiber zerumbet is investigated for the bioconversion of the monoterpene precursors geranyl diphosphate (2E-GPP) and neryl diphosphate (2Z-NPP) as well as for the sesquiterpene precursor farnesyl diphosphate (2E,6E-FPP)
additional information
the enzyme contains conserved RPx8W motif and DDxxD motif, which is a divalent metal-ion substrate-binding site
additional information
the enzyme contains conserved RPx8W motif and DDxxD motif, which is a divalent metal-ion substrate-binding site
additional information
the enzyme contains conserved RPx8W motif and DDxxD motif, which is a divalent metal-ion substrate-binding site
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ASR6_SARSH
Sarocladium schorii
430
0
48387
Swiss-Prot
other Location (Reliability: 2)
TATC6_HYPAT
386
0
44939
Swiss-Prot
Mitochondrion (Reliability: 3)
TPS2_TOOSI
556
0
64552
Swiss-Prot
other Location (Reliability: 5)
CS_HELAN
555
0
64263
Swiss-Prot
other Location (Reliability: 4)
STS1_HUMLU
563
0
66219
Swiss-Prot
other Location (Reliability: 4)
BCPSD_ORIVU
554
0
64724
Swiss-Prot
other Location (Reliability: 4)
BCPSF_ORIVU
554
0
64662
Swiss-Prot
other Location (Reliability: 4)
EUPE_PHOSX
Phoma sp
379
0
43179
Swiss-Prot
other Location (Reliability: 1)
ZSS1_ZINZE
548
0
64518
Swiss-Prot
other Location (Reliability: 2)
TPS1_PIPNI
559
0
64936
Swiss-Prot
Secretory Pathway (Reliability: 5)
TPS12_SOLHA
545
0
63985
Swiss-Prot
other Location (Reliability: 3)
TPS12_SOLLC
548
0
64321
Swiss-Prot
other Location (Reliability: 5)
AHUMS_PICGL
575
0
66334
Swiss-Prot
other Location (Reliability: 2)
A0A251T2E0_HELAN
555
0
64263
TrEMBL
other Location (Reliability: 4)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Sarocladium sp. ''schorii
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
the enzyme has no N-terminal transit peptide
additional information
the enzyme has no N-terminal transit peptide
additional information
the enzyme has no N-terminal transit peptide
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
heterologous expression of the mevalonate pathway and alpha-humulene synthase results in the production of alpha-humulene from CO2 under heterotrophic conditions. The production system is used to produce 17 mg alpha-humulene per gram cell dry mass (CDW) from CO2 and electrical energy in microbial electrosynthesis (MES) mode
additional information
alpha-humulene production using the engineered Escherichia coli BL21(DE3) strain in a 2-l bioreactor cultivation, method optimization, overview. Coordinating Cu2+ ions to the NTA ligands in affinity chromatography results in a high non-specific binding of endogenous host cell proteins due to the high affinity of Cu2+ to histidine groups. Zn2+-IMAC shows much less impurities in eluted protein fractions which is consistent with the fact that Zn2+ ions are less affine to histidine groups. The recovery rate of 80 % of the protein of interest using Zn2+ ions is the lowest compared to all other metal ions tested. Ni2+- and Co2+-IMAC seem to be similar in product purity (60% and 63%) and recovery (93% and 95%). As Co2+-IMAC shows the best purity to recovery ratio, further purification is done using Co2+-IMAC
additional information
de novo production of zerumbone is achieved in a metabolically engineered yeast cell factory by introducing alpha-humulene synthase (ZSS1), alpha-humulene 8-hydroxylase (CYP71BA1) and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana into the Saccharomyces cerevisiae strain. Multistep metabolic engineering strategies are applied, including the overexpression of the mevalonate (MVA) pathway rate-limiting enzymes tHMG1 and yeast ERG20, regulation of yeast ERG9 by an inducible promoter and competitive pathway deletion to redirect metabolic flux toward the desired product, method evaluation and optimization, overview. In the engineered strain, alpha-humulene production increases by 18fold, to 92 mg/l compared to that in the original strain, fed-batch fermentation using Saccharomyces cerevisiae strain LW16 in a 5-l bioreactor
additional information
potential of Methylobacterium extorquens as a future platform host for the production of high-value terpenoids from the alternative carbon source methanol. Inducible recombinant coexpression of alpha-humulene synthasefrom Zingiber zerumbet with farnesyl diphosphate (FPP) synthase from Saccharomyces cerevisiae leads to concentrations of up to 18 mg/l alpha-humulene in Methylobacterium extorquens strain AM1. Extracellular alpha-humulene has no growth inhibiting effect on Methylobacterium extorquens strain AM1 up to concentrations of 1 g/l. Method evaluation, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)by cobalt affinity chromatography, dialysis, and ultrafiltration, to homogeneity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene HS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli
gene HS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli
gene HS3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli
gene ZSS1 or hum, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) under control of the lac promoter
gene ZSS1 or HUM, recombinant His-tagged enzyme expression from codon-optimized alpha-humulene synthase gene in Escherichia coli strain BL21(DE3)
gene zssI, recombinant coexpression of alpha-humulene synthase of Zingiber zerumbet from codon-optimized gene zssI with farnesyl diphosphate (FPP) synthase from Saccharomyces cerevisiae leads to concentrations of up to 18 mg/l alpha-humulene in Methylobacterium extorquens strain AM1, cloning scheme comprising genes zssI and ERG20
gene ZSSI, recombinant coexpression of alpha-humulene synthase of Zingiber zerumbet with alpha-humulene 8-hydroxylase (CYP71BA1) and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana in Saccharomyces cerevisiae strain LW16, subcloning and expression in Escherichia coli strain trans-T1
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Diprion pini oviposition enhances the transcription of sesquiterpene synthase TPS1 by 17.5fold. The transcripts TPS1 and TPS2 are induced in combinations of pine and sawfly species only when the egg-laden needles are attractive to Closterocerus ruforum, which parasitizes sawfly eggs
-
expression is induced upon wounding or methyljasmonate treatment
in the presence of 1 mg/ml lithium acetoacetate, a 13.6fold increase in alpha-humulene production occurs compared with the control
-
methyl jasmonate slightly induces the expression of enzyme alpha-humulene synthase, at 6 h after methyl jasmonate treatment, the expression of the alpha-humulene synthase gene has increased approximately 1.5fold resulting in 330 pmol/mg protein/h alpha-humulene production
the enzyme is up-regulated in both leaves and rhizomes after treatment with methyl jasmonate
-
the transcript of ZZS1 gene increases very rapidly from June 12 to June 28, a period of strong temperature increase in 2007. Transcript level remains high throughout the summer and autumn, but declines significantly in December
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
-
chemolithoautotrophic production of a terpene from carbon dioxide, hydrogen, and oxygen, involving the engineered Cupriavidus necator cells and enzyme alpha-humulene synthase, is a promising starting point for the production of different high-value terpene compounds from abundant and simple raw materials. The production system is used to produce 17 mg alpha-humulene per gram cell dry mass (CDW) from CO2 and electrical energy in microbial electrosynthesis (MES) mode
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Harada, H.; Yu, F.; Okamoto, S.; Kuzuyama, T.; Utsumi, R.; Misawa, N.
Efficient synthesis of functional isoprenoids from acetoacetate through metabolic pathway-engineered Escherichia coli
Appl. Microbiol. Biotechnol.
81
915-925
2008
Zingiber zerumbet
brenda
van Der Hoeven, R.S.; Monforte, A.J.; Breeden, D.; Tanksley, S.D.; Steffens, J.C.
Genetic control and evolution of sesquiterpene biosynthesis in Lycopersicon esculentum and L. hirsutum
Plant Cell
12
2283-2294
2000
Solanum lycopersicum, no activity in Solanum habrochaites
brenda
Koepke, D.; Beyaert, I.; Gershenzon, J.; Hilker, M.; Schmidt, A.
Species-specific responses of pine sesquiterpene synthases to sawfly oviposition
Phytochemistry
71
909-917
2010
Pinus sylvestris
brenda
Bleeker, P.M.; Spyropoulou, E.A.; Diergaarde, P.J.; Volpin, H.; De Both, M.T.; Zerbe, P.; Bohlmann, J.; Falara, V.; Matsuba, Y.; Pichersky, E.; Haring, M.A.; Schuurink, R.C.
RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes
Plant Mol. Biol.
77
323-336
2011
Solanum habrochaites (G8H5M8)
brenda
Yu, F.; Okamoto, S.; Harada, H.; Yamasaki, K.; Misawa, N.; Utsumi, R.
Zingiber zerumbet CYP71BA1 catalyzes the conversion of ?-humulene to 8-hydroxy-alpha-humulene in zerumbone biosynthesis
Cell. Mol. Life Sci.
68
1033-1040
2011
Zingiber zerumbet
brenda
Schilmiller, A.L.; Miner, D.P.; Larson, M.; McDowell, E.; Gang, D.R.; Wilkerson, C.; Last, R.L.
Studies of a biochemical factory: tomato trichome deep expressed sequence tag sequencing and proteomics
Plant Physiol.
153
1212-1223
2010
Solanum lycopersicum (D5KXD2)
brenda
Yu, F.; Okamto, S.; Nakasone, K.; Adachi, K.; Matsuda, S.; Harada, H.; Misawa, N.; Utsumi, R.
Molecular cloning and functional characterization of alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis in shampoo ginger (Zingiber zerumbet Smith)
Planta
227
1291-1299
2008
Zingiber zerumbet
brenda
Yang, C.Q.; Wu, X.M.; Ruan, J.X.; Hu, W.L.; Mao, Y.B.; Chen, X.Y.; Wang, L.J.
Isolation and characterization of terpene synthases in cotton (Gossypium hirsutum)
Phytochemistry
96
46-56
2013
Gossypium hirsutum (K7PRF2), Gossypium hirsutum (U5N1F1)
brenda
Krieg, T.; Sydow, A.; Faust, S.; Huth, I.; Holtmann, D.
CO2 to terpenes autotrophic and electroautotrophic alpha-humulene production with Cupriavidus necator
Angew. Chem. Int. Ed. Engl.
57
1879-1882
2018
Cupriavidus necator
brenda
Alemdar, S.; Hartwig, S.; Frister, T.; Koenig, J.C.; Scheper, T.; Beutel, S.
Heterologous expression, purification, and biochemical characterization of alpha-humulene synthase from Zingiber zerumbet Smith
Appl. Biochem. Biotechnol.
178
474-489
2016
Zingiber zerumbet (B1B1U3)
brenda
Alemdar, S.; Koenig, J.; Hartwig, S.; Frister, T.; Scheper, T.; Beutel, S.
Bioproduction of alpha-humulene in metabolically engineered Escherichia coli and application in zerumbone synthesis
Eng. Life Sci.
17
900-907
2017
Zingiber zerumbet (B1B1U3)
-
brenda
Kumeta, Y.; Ito, M.
Characterization of alpha-humulene synthases responsible for the production of sesquiterpenes induced by methyl jasmonate in Aquilaria cell culture
J. Nat. Med.
70
452-459
2016
Aquilaria crassna (A0A142F308), Aquilaria crassna (A0A142F309), Aquilaria crassna (A0A142F310)
brenda
Sonntag, F.; Kroner, C.; Lubuta, P.; Peyraud, R.; Horst, A.; Buchhaupt, M.; Schrader, J.
Engineering Methylobacterium extorquens for de novo synthesis of the sesquiterpenoid alpha-humulene from methanol
Metab. Eng.
32
82-94
2015
Zingiber zerumbet (B1B1U3)
brenda
Zhang, C.; Liu, J.; Zhao, F.; Lu, C.; Zhao, G.R.; Lu, W.
Production of sesquiterpenoid zerumbone from metabolic engineered Saccharomyces cerevisiae
Metab. Eng.
49
28-35
2018
Zingiber zerumbet (B1B1U3)
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 4.2.3.104)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
Server: brenda5