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Literature summary for 4.2.3.104 extracted from

  • Alemdar, S.; Koenig, J.; Hartwig, S.; Frister, T.; Scheper, T.; Beutel, S.
    Bioproduction of alpha-humulene in metabolically engineered Escherichia coli and application in zerumbone synthesis (2017), Eng. Life Sci., 17, 900-907 .
No PubMed abstract available
Search for more literature for 4.2.3.104

Cloned(Commentary)

Cloned (Comment) Organism
gene ZSS1 or HUM, recombinant His-tagged enzyme expression from codon-optimized alpha-humulene synthase gene in Escherichia coli strain BL21(DE3) Zingiber zerumbet

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Zingiber zerumbet
0.0303
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 38°C, recombinant His-tagged enzyme Zingiber zerumbet
0.068
-
 (2Z)-neryl diphosphate pH 7.5, 38°C, recombinant His-tagged enzyme Zingiber zerumbet
0.0736
-
 (2E)-geranyl diphosphate pH 7.5, 38°C, recombinant His-tagged enzyme Zingiber zerumbet

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Zingiber zerumbet
-
 alpha-humulene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Zingiber zerumbet B1B1U3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography Zingiber zerumbet

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.45
-
 substrate (2E)-geranyl diphosphate, pH 7.5, 38°C, recombinant His-tagged enzyme Zingiber zerumbet
3.81
-
 substrate (2Z)-neryl diphosphate, pH 7.5, 38°C, recombinant His-tagged enzyme Zingiber zerumbet
5.08
-
 substrate (2E,6E)-farnesyl diphosphate, pH 7.5, 38°C, recombinant His-tagged enzyme Zingiber zerumbet

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-geranyl diphosphate + H2O the enzyme produces limonene, linalool, alpha-terpineol, and geraniol from geranyl diphosphate. Geraniol is the main product Zingiber zerumbet geraniol + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Zingiber zerumbet alpha-humulene + diphosphate
-
 ?
(2Z)-neryl diphosphate the enzyme produces linalool, alpha-terpineol, geraniol, alpha-terpinolene, p-menth-2-en-1-ol, piperitol, and nerol from neryl diphosphate. Limonene is the main product Zingiber zerumbet limonene + diphosphate
-
 ?
additional information malachite green assay adapted to rapidly assay terpene synthase activity for activity determination, and GS-MS analysis Zingiber zerumbet ?
-
 ?

Synonyms

Synonyms Comment Organism
hum
-
 Zingiber zerumbet
ZSS1
-
 Zingiber zerumbet

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 recombinant enzyme Zingiber zerumbet

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 (2E)-geranyl diphosphate pH 7.5, 38°C, recombinant His-tagged enzyme Zingiber zerumbet
4.26
-
 (2Z)-neryl diphosphate pH 7.5, 38°C, recombinant His-tagged enzyme Zingiber zerumbet
5.61
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 38°C, recombinant His-tagged enzyme Zingiber zerumbet

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 recombinant enzyme Zingiber zerumbet

General Information

General Information Comment Organism
additional information sesquiterpene synthase alpha-humulene synthase (HUM) from Zingiber zerumbet is investigated for the bioconversion of the monoterpene precursors geranyl diphosphate (2E-GPP) and neryl diphosphate (2Z-NPP) as well as for the sesquiterpene precursor farnesyl diphosphate (2E,6E-FPP) Zingiber zerumbet