We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
l-kda dehydratase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-keto-3-deoxy-L-arabinonate dehydratase
-
-
-
dehydratase, 2-keto-3-deoxyL-arabonate
-
-
-
L-2-keto-3-deoxyarabonate dehydratase
L-2-keto-3-deoxyarabonate dehydratase
-
-
-
L-2-keto-3-deoxyarabonate dehydratase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-dehydro-3-deoxy-L-arabinonate = 2,5-dioxopentanoate + H2O
2-dehydro-3-deoxy-L-arabinonate = 2,5-dioxopentanoate + H2O
Schiff base intermediate
-
2-dehydro-3-deoxy-L-arabinonate = 2,5-dioxopentanoate + H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-dehydro-3-deoxy-L-arabinonate hydro-lyase (2,5-dioxopentanoate-forming)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-dehydro-3-deoxy-L-arabinonate
2,5-dioxopentanoate + H2O
-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DL-2-keto-3-hydroxypentanoate
-
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
4 mM, 50% inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.07
2-dehydro-3-deoxy-L-arabinonate
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4
p-hydroxymercuribenzoate
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.8 - 9.2
-
less than 50% of maximal activity above and below
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
-
-
-
brenda
-
-
-
brenda
MNF300
-
-
brenda
MNF300
-
-
brenda
NGR234
-
-
brenda
NGR234
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
35000
2 * 35000, SDS-PAGE
36000
2 * 36000, SDS-PAGE
85000
-
gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimer
2 * 35000, SDS-PAGE
dimer
2 * 36000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
crystallized at 18°C using the hanging-drop vapour-diffusion method. The crystal diffracts to 2.0 A resolution using synchrotron radiation and belongs to the trigonal space group P3(1)2(1) or its enantiomorph P3(2)2(1), with unit-cell parameters a = b = 78.91, c = 207.71 A
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Q143E
inactive mutant enzyme
Q143N
inactive mutant enzyme
Q143S
inactive mutant enzyme
Q143T
inactive mutant enzyme
Q143Y
inactive mutant enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4°C, 10 mM phosphate buffer, pH 7.5, 20% (NH4)2SO4
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
wild-type and mutant enzymes Q143N, Q143E, Q143S, Q143T, and Q143Y
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed as a N-terminal His6-tagged protein in Escherichia coli
mutant enzymes Q143N, Q143E, Q143S, Q143T, and Q143Y are overexpressed in Escherichia coli cells as a His6-tagged enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Stoolmiller, A.C.
L-2-Keto-3-deoxyarabonate dehydratase
Methods Enzymol.
42C
308-315
1975
Pelomonas saccharophila
brenda
Portsmouth, D.; Stoolmiller, A.C.; Abeles, R.H.
Studies on the mechanism of action of 2-keto-3-deoxy-L-arabonate dehydratase. The participation of an enzyme-substrate Schiff base in a dehydration
J. Biol. Chem.
242
2751-2759
1967
Pelomonas saccharophila
brenda
Stoolmiller, A.C.; Abeles, R.H.
Formation of alpha-ketoglutaric semialdehyde from L-2-keto-3-deoxyarabonic acid and isolation of L-2-keto-3-deoxyarabonate dehydratase from Pseudomonas saccharophila
J. Biol. Chem.
241
5764-5771
1966
Pelomonas saccharophila
brenda
Dilworth, M.J.; Arwas, R.; McKay, I.A.; Saroso, S.; Glenn, A.R.
Pentose metabolism in Rhizobium leguminosarum MNF300 and in cowpea Rhizobium NGR234
J. Gen. Microbiol.
132
2733-2742
1986
Rhizobium leguminosarum, Rhizobium sp., Rhizobium leguminosarum MNF300, Rhizobium sp. NGR234
-
brenda
Mathias, A.L.; un Rigo, L.; Funayama, S.; Pedrosa, F.O.
L-Arabinose metabolism in Herbaspirillum seropedicae
J. Bacteriol.
171
5206-5209
1989
Herbaspirillum seropedicae
brenda
Watanabe, S.; Shimada, N.; Tajima, K.; Kodaki, T.; Makino, K.
Identification and Characterization of L-Arabonate Dehydratase, L-2-Keto-3-deoxyarabonate Dehydratase, and L-Arabinolactonase Involved in an Alternative Pathway of L-Arabinose Metabolism: Novel Evolution ary Insight into Sugar Metabolism
J. Biol. Chem.
281
33521-33536
2006
Azospirillum brasilense (Q1JUQ0), Azospirillum brasilense
brenda
Shimada, N.; Mikami, B.; Watanabe, S.; Makino, K.
Preliminary crystallographic analysis of L-2-keto-3-deoxyarabonate dehydratase, an enzyme involved in an alternative bacterial pathway of L-arabinose metabolism
Acta Crystallogr. Sect. F
63
393-395
2007
Azospirillum brasilense (Q1JUQ0)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
4.2.1.43 2-dehydro-3-deoxy-L-arabinonate dehydratase
more
top
Information
