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Information on EC 4.2.1.43 - 2-dehydro-3-deoxy-L-arabinonate dehydratase for references in articles please use BRENDA:EC4.2.1.43
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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
2-keto-3-deoxy-L-arabinonate dehydratase, dehydratase, 2-keto-3-deoxyL-arabonate, KDA dehydratase, L-2-keto-3-deoxyarabonate dehydratase, L-KDA dehydratase,
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2-keto-3-deoxy-L-arabinonate dehydratase
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dehydratase, 2-keto-3-deoxyL-arabonate
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L-2-keto-3-deoxyarabonate dehydratase
L-2-keto-3-deoxyarabonate dehydratase
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L-2-keto-3-deoxyarabonate dehydratase
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2-dehydro-3-deoxy-L-arabinonate = 2,5-dioxopentanoate + H2O
2-dehydro-3-deoxy-L-arabinonate = 2,5-dioxopentanoate + H2O
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2-dehydro-3-deoxy-L-arabinonate = 2,5-dioxopentanoate + H2O
Schiff base intermediate
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2-dehydro-3-deoxy-L-arabinonate hydro-lyase (2,5-dioxopentanoate-forming)
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2-dehydro-3-deoxy-L-arabinonate
2,5-dioxopentanoate + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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DL-2-keto-3-hydroxypentanoate
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p-hydroxymercuribenzoate
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p-hydroxymercuribenzoate
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4 mM, 50% inhibition
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0.07
2-dehydro-3-deoxy-L-arabinonate
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4
p-hydroxymercuribenzoate
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5.8 - 9.2
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less than 50% of maximal activity above and below
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SwissProt
brenda
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brenda
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brenda
MNF300
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brenda
MNF300
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brenda
NGR234
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brenda
NGR234
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brenda
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35000
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2 * 35000, SDS-PAGE
36000
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2 * 36000, SDS-PAGE
85000
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gel filtration
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dimer
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2 * 36000, SDS-PAGE
dimer
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2 * 35000, SDS-PAGE
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crystallized at 18°C using the hanging-drop vapour-diffusion method. The crystal diffracts to 2.0 A resolution using synchrotron radiation and belongs to the trigonal space group P3(1)2(1) or its enantiomorph P3(2)2(1), with unit-cell parameters a = b = 78.91, c = 207.71 A
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Q143E
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inactive mutant enzyme
Q143N
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inactive mutant enzyme
Q143S
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inactive mutant enzyme
Q143T
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inactive mutant enzyme
Q143Y
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inactive mutant enzyme
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4°C, 10 mM phosphate buffer, pH 7.5, 20% (NH4)2SO4
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wild-type and mutant enzymes Q143N, Q143E, Q143S, Q143T, and Q143Y
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expressed as a N-terminal His6-tagged protein in Escherichia coli
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mutant enzymes Q143N, Q143E, Q143S, Q143T, and Q143Y are overexpressed in Escherichia coli cells as a His6-tagged enzyme
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Stoolmiller, A.C.
L-2-Keto-3-deoxyarabonate dehydratase
Methods Enzymol.
42C
308-315
1975
Pelomonas saccharophila
brenda
Portsmouth, D.; Stoolmiller, A.C.; Abeles, R.H.
Studies on the mechanism of action of 2-keto-3-deoxy-L-arabonate dehydratase. The participation of an enzyme-substrate Schiff base in a dehydration
J. Biol. Chem.
242
2751-2759
1967
Pelomonas saccharophila
brenda
Stoolmiller, A.C.; Abeles, R.H.
Formation of alpha-ketoglutaric semialdehyde from L-2-keto-3-deoxyarabonic acid and isolation of L-2-keto-3-deoxyarabonate dehydratase from Pseudomonas saccharophila
J. Biol. Chem.
241
5764-5771
1966
Pelomonas saccharophila
brenda
Dilworth, M.J.; Arwas, R.; McKay, I.A.; Saroso, S.; Glenn, A.R.
Pentose metabolism in Rhizobium leguminosarum MNF300 and in cowpea Rhizobium NGR234
J. Gen. Microbiol.
132
2733-2742
1986
Rhizobium leguminosarum, Rhizobium leguminosarum MNF300, Rhizobium sp., Rhizobium sp. NGR234
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brenda
Mathias, A.L.; un Rigo, L.; Funayama, S.; Pedrosa, F.O.
L-Arabinose metabolism in Herbaspirillum seropedicae
J. Bacteriol.
171
5206-5209
1989
Herbaspirillum seropedicae
brenda
Watanabe, S.; Shimada, N.; Tajima, K.; Kodaki, T.; Makino, K.
Identification and Characterization of L-Arabonate Dehydratase, L-2-Keto-3-deoxyarabonate Dehydratase, and L-Arabinolactonase Involved in an Alternative Pathway of L-Arabinose Metabolism: Novel Evolution ary Insight into Sugar Metabolism
J. Biol. Chem.
281
33521-33536
2006
Azospirillum brasilense, Azospirillum brasilense (Q1JUQ0)
brenda
Shimada, N.; Mikami, B.; Watanabe, S.; Makino, K.
Preliminary crystallographic analysis of L-2-keto-3-deoxyarabonate dehydratase, an enzyme involved in an alternative bacterial pathway of L-arabinose metabolism
Acta Crystallogr. Sect. F
63
393-395
2007
Azospirillum brasilense (Q1JUQ0)
brenda
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4.2.1.43 2-dehydro-3-deoxy-L-arabinonate dehydratase
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