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Information on EC 4.2.1.43 - 2-dehydro-3-deoxy-L-arabinonate dehydratase for references in articles please use BRENDA:EC4.2.1.43
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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
l-kda dehydratase,
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2-keto-3-deoxy-L-arabinonate dehydratase
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dehydratase, 2-keto-3-deoxyL-arabonate
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-
-
-
L-2-keto-3-deoxyarabonate dehydratase
L-2-keto-3-deoxyarabonate dehydratase
-
-
-
-
L-2-keto-3-deoxyarabonate dehydratase
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2-dehydro-3-deoxy-L-arabinonate = 2,5-dioxopentanoate + H2O
2-dehydro-3-deoxy-L-arabinonate = 2,5-dioxopentanoate + H2O
Schiff base intermediate
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2-dehydro-3-deoxy-L-arabinonate = 2,5-dioxopentanoate + H2O
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2-dehydro-3-deoxy-L-arabinonate hydro-lyase (2,5-dioxopentanoate-forming)
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2-dehydro-3-deoxy-L-arabinonate
2,5-dioxopentanoate + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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-
-
-
?
L-2-keto-3-deoxyarabonate
2-oxoglutarate semialdehyde + H2O
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-
-
-
?
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DL-2-keto-3-hydroxypentanoate
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p-hydroxymercuribenzoate
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p-hydroxymercuribenzoate
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4 mM, 50% inhibition
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0.07
2-dehydro-3-deoxy-L-arabinonate
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-
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4
p-hydroxymercuribenzoate
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-
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5.8 - 9.2
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less than 50% of maximal activity above and below
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SwissProt
brenda
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-
-
brenda
-
-
-
brenda
MNF300
-
-
brenda
MNF300
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-
brenda
NGR234
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-
brenda
NGR234
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-
brenda
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KDADA_AZOBR
309
0
33756
Swiss-Prot
A0A2N9CZC7_9BURK
309
0
33847
TrEMBL
A0A2N9BKG2_STRCX
311
0
33167
TrEMBL
A0A2C9D040_9RHIZ
310
0
33392
TrEMBL
A0A1Y5R973_9RHOB
305
0
32888
TrEMBL
A1WLE2_VEREI
Verminephrobacter eiseniae (strain EF01-2)
316
0
34443
TrEMBL
A0A160TSH9_9ZZZZ
43
0
4889
TrEMBL
A0A143PR56_9BACT
302
0
31817
TrEMBL
Q0B5V1_BURCM
Burkholderia ambifaria (strain ATCC BAA-244 / AMMD)
309
0
33834
TrEMBL
A5G247_ACICJ
Acidiphilium cryptum (strain JF-5)
309
0
33555
TrEMBL
A0A0C4YND7_9BURK
308
0
33303
TrEMBL
A0A1V5ZBG7_9BACT
315
0
34701
TrEMBL
A0A0P1EYT7_9RHOB
303
0
32902
TrEMBL
A0A0H2MLS8_VARPD
305
0
33476
TrEMBL
A0A1X6ZAF6_9RHOB
305
0
32845
TrEMBL
A0A1X6ZW84_9RHOB
307
0
32923
TrEMBL
A0A375CK34_9BURK
310
0
33917
TrEMBL
A0A127MTP7_9PSED
312
0
34445
TrEMBL
A0A1M4MYE7_9RHOB
304
0
32991
TrEMBL
A0A399E8S5_9DEIN
305
0
33141
TrEMBL
A0A399HGE6_9ACTN
305
0
31784
TrEMBL
A0A238L6W0_9RHOB
305
0
32778
TrEMBL
A0A238KJL2_9RHOB
305
0
33059
TrEMBL
A0A2Z5KDY5_9ACTN
311
0
33409
TrEMBL
A0A375HGQ9_9BURK
310
0
33961
TrEMBL
A0A1R3TXM2_9RHIZ
302
0
31670
TrEMBL
A0A238HBF6_9BURK
308
0
33438
TrEMBL
A0A066PRX8_9PROT
309
0
33654
TrEMBL
A0A0P0RKT1_9BURK
309
0
33970
TrEMBL
A0A1N7SQ25_9BURK
310
0
33421
TrEMBL
A0A2S6TPE8_9PROT
195
0
20910
TrEMBL
A0A1E3H6A0_9RHIZ
317
0
34047
TrEMBL
Q129P0_POLSJ
Polaromonas sp. (strain JS666 / ATCC BAA-500)
305
0
33259
TrEMBL
A0A2N9CQR4_9BURK
305
0
33096
TrEMBL
A0A1J5PHE3_9ZZZZ
308
0
33346
TrEMBL
A0A2S6U5U9_9PROT
305
0
33206
TrEMBL
A0A1U9ZAE5_9RHIZ
301
0
32587
TrEMBL
A0A0H2Y0S3_BURCA
Burkholderia cenocepacia (strain AU 1054)
309
0
33775
TrEMBL
A0A2U3QCC2_9BRAD
312
0
33965
TrEMBL
R0CIN7_RALPI
313
0
34064
TrEMBL
A0A1Y0NBJ2_9BURK
309
0
33498
TrEMBL
J7JLG7_BURCE
309
0
33731
TrEMBL
A0A2K8KQP9_9GAMM
299
0
33088
TrEMBL
A0A1Y6KL25_9BRAD
309
0
33671
TrEMBL
A0A1R3TC66_9RHIZ
308
0
32888
TrEMBL
A0A1Y0EGF7_9RHOB
305
0
32841
TrEMBL
A0A2Z4AF00_9BACT
319
0
34419
TrEMBL
A0A1Y5T5S7_9RHOB
308
0
32290
TrEMBL
A0A066PK49_9PROT
308
0
33539
TrEMBL
A0A2S6U6V9_9PROT
312
0
34205
TrEMBL
A0A1N7RPD8_9BURK
309
0
33780
TrEMBL
A1BB33_PARDP
Paracoccus denitrificans (strain Pd 1222)
310
0
33525
TrEMBL
W8WZW0_CASDE
311
0
33854
TrEMBL
A0A238L4B1_9RHOB
305
0
33170
TrEMBL
A0A0H2MCA9_VARPD
312
0
34408
TrEMBL
A0A2Z6I1L2_9RHIZ
310
0
32869
TrEMBL
A0A068YX91_9BURK
160
0
17585
TrEMBL
A0A2X1FCB2_9BURK
309
0
33817
TrEMBL
A0A1K0IPK2_CUPNE
310
0
33817
TrEMBL
A0A238JFR7_9RHOB
305
0
33201
TrEMBL
A0A1E1UVZ1_9BRAD
305
0
33170
TrEMBL
A0A0P1ISX5_9RHOB
304
0
33229
TrEMBL
A0A2Z4AFM4_9BACT
303
0
33772
TrEMBL
A0A0F0KZY1_9MICO
305
0
31858
TrEMBL
A0A102UAW7_BURCE
309
0
33901
TrEMBL
A0A395KZN4_9RHIZ
310
0
34002
TrEMBL
A0A2H5YVI5_9BACT
309
0
32845
TrEMBL
A0A0P1G8J5_9RHOB
303
0
32902
TrEMBL
A0A0M6XYS3_9RHOB
303
0
33073
TrEMBL
A0A1Y2MXD6_PSEAH
302
0
31024
TrEMBL
A1TPD7_ACIAC
Acidovorax citrulli (strain AAC00-1)
312
0
34228
TrEMBL
G7ZHJ9_AZOL4
Azospirillum lipoferum (strain 4B)
339
0
36693
TrEMBL
A0A1N7RNM8_9BURK
309
0
33854
TrEMBL
A0A2R8AQ32_9RHOB
304
0
33063
TrEMBL
A0A1Y5TIC9_9RHOB
313
0
32588
TrEMBL
A0A142XMX1_9BACT
305
0
32635
TrEMBL
A0A0N7LNK9_9RHOB
304
0
33102
TrEMBL
Q21ZN5_RHOFT
Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)
309
0
33667
TrEMBL
Q221U8_RHOFT
Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)
309
0
33748
TrEMBL
A0A1Q2YU49_9PROT
309
0
34029
TrEMBL
A0A1X7A572_9RHOB
305
0
33326
TrEMBL
Q39BK2_BURL3
Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)
309
0
33764
TrEMBL
A0A177H9T0_9RHOB
303
0
33093
TrEMBL
A4JKL4_BURVG
Burkholderia vietnamiensis (strain G4 / LMG 22486)
309
0
33708
TrEMBL
A0A1V5E9S1_9DELT
307
0
32664
TrEMBL
A0A0F0LT28_9MICO
305
0
31356
TrEMBL
A0A3N6E2V6_9ACTN
301
0
31088
TrEMBL
A0A1E3ACA7_9FIRM
303
0
33905
TrEMBL
A0A0S3PZW6_9BRAD
306
0
32783
TrEMBL
A0A068YT63_9BURK
72
0
7536
TrEMBL
A0A1V0Q126_9RHOB
305
0
32930
TrEMBL
A0A0U1NPJ9_9RHOB
247
0
27177
TrEMBL
A0A386UQL2_9RHOB
303
0
32688
TrEMBL
A0A1P8WPG8_9PLAN
302
0
32666
TrEMBL
A0A375HS36_9BURK
310
0
33877
TrEMBL
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35000
-
2 * 35000, SDS-PAGE
36000
-
2 * 36000, SDS-PAGE
85000
-
gel filtration
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dimer
-
2 * 36000, SDS-PAGE
dimer
-
2 * 35000, SDS-PAGE
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crystallized at 18°C using the hanging-drop vapour-diffusion method. The crystal diffracts to 2.0 A resolution using synchrotron radiation and belongs to the trigonal space group P3(1)2(1) or its enantiomorph P3(2)2(1), with unit-cell parameters a = b = 78.91, c = 207.71 A
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Q143E
-
inactive mutant enzyme
Q143N
-
inactive mutant enzyme
Q143S
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inactive mutant enzyme
Q143T
-
inactive mutant enzyme
Q143Y
-
inactive mutant enzyme
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4°C, 10 mM phosphate buffer, pH 7.5, 20% (NH4)2SO4
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wild-type and mutant enzymes Q143N, Q143E, Q143S, Q143T, and Q143Y
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expressed as a N-terminal His6-tagged protein in Escherichia coli
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mutant enzymes Q143N, Q143E, Q143S, Q143T, and Q143Y are overexpressed in Escherichia coli cells as a His6-tagged enzyme
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Stoolmiller, A.C.
L-2-Keto-3-deoxyarabonate dehydratase
Methods Enzymol.
42C
308-315
1975
Pelomonas saccharophila
brenda
Portsmouth, D.; Stoolmiller, A.C.; Abeles, R.H.
Studies on the mechanism of action of 2-keto-3-deoxy-L-arabonate dehydratase. The participation of an enzyme-substrate Schiff base in a dehydration
J. Biol. Chem.
242
2751-2759
1967
Pelomonas saccharophila
brenda
Stoolmiller, A.C.; Abeles, R.H.
Formation of alpha-ketoglutaric semialdehyde from L-2-keto-3-deoxyarabonic acid and isolation of L-2-keto-3-deoxyarabonate dehydratase from Pseudomonas saccharophila
J. Biol. Chem.
241
5764-5771
1966
Pelomonas saccharophila
brenda
Dilworth, M.J.; Arwas, R.; McKay, I.A.; Saroso, S.; Glenn, A.R.
Pentose metabolism in Rhizobium leguminosarum MNF300 and in cowpea Rhizobium NGR234
J. Gen. Microbiol.
132
2733-2742
1986
Rhizobium leguminosarum, Rhizobium leguminosarum MNF300, Rhizobium sp., Rhizobium sp. NGR234
-
brenda
Mathias, A.L.; un Rigo, L.; Funayama, S.; Pedrosa, F.O.
L-Arabinose metabolism in Herbaspirillum seropedicae
J. Bacteriol.
171
5206-5209
1989
Herbaspirillum seropedicae
brenda
Watanabe, S.; Shimada, N.; Tajima, K.; Kodaki, T.; Makino, K.
Identification and Characterization of L-Arabonate Dehydratase, L-2-Keto-3-deoxyarabonate Dehydratase, and L-Arabinolactonase Involved in an Alternative Pathway of L-Arabinose Metabolism: Novel Evolution ary Insight into Sugar Metabolism
J. Biol. Chem.
281
33521-33536
2006
Azospirillum brasilense, Azospirillum brasilense (Q1JUQ0)
brenda
Shimada, N.; Mikami, B.; Watanabe, S.; Makino, K.
Preliminary crystallographic analysis of L-2-keto-3-deoxyarabonate dehydratase, an enzyme involved in an alternative bacterial pathway of L-arabinose metabolism
Acta Crystallogr. Sect. F
63
393-395
2007
Azospirillum brasilense (Q1JUQ0)
brenda
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4.2.1.43 2-dehydro-3-deoxy-L-arabinonate dehydratase
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