Literature summary for 4.2.1.43 extracted from

Stoolmiller, A.C.; Abeles, R.H.
Formation of alpha-ketoglutaric semialdehyde from L-2-keto-3-deoxyarabonic acid and isolation of L-2-keto-3-deoxyarabonate dehydratase from Pseudomonas saccharophila (1966), J. Biol. Chem., 241, 5764-5771.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Pelomonas saccharophila

Inhibitors

Inhibitors	Comment	Organism	Structure
p-hydroxymercuribenzoate	4 mM, 50% inhibition	Pelomonas saccharophila

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.07	-	2-dehydro-3-deoxy-L-arabinonate	-	Pelomonas saccharophila

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
85000	-	gel filtration	Pelomonas saccharophila

Organism

Organism	UniProt	Comment	Textmining
Pelomonas saccharophila	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pelomonas saccharophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
54	-	-	Pelomonas saccharophila

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-dehydro-3-deoxy-L-arabinonate	-	Pelomonas saccharophila	2,5-dioxopentanoate + H2O	-	?
L-2-keto-3-deoxyarabonate	-	Pelomonas saccharophila	2-oxoglutarate semialdehyde + H2O	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	-	Pelomonas saccharophila

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4	-	p-hydroxymercuribenzoate	-	Pelomonas saccharophila

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Release 2023.1 (January 2023)