crude cell-extract completely loses activity in open tubes in ice bath for 2-4 h, activity restored after incubation at 25°C for 30 min, extent of activation decreases with each succeeding cycle of inactivation and reactivation, loss of activity after treatment with EDTA or after dialysis
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expression of plasmid-encoded ttdA'-'lacZ is stimulated to a high level (64fold) by L-tartrate during anaerobic growth. meso-Tartrate causes slightly lower (47.5fold) induction. During aerobic growth, expression of ttdA'-'lacZ is very low in the presence or absence of L-tartrate, and it increases by a factor of about 220 under anaerobic conditions. Expression of ttdA requires transcriptional activation by fumarate nitrate reductase regulator (FNR), which is in the active state only under anaerobic conditions
expression of ttdA is repressed by O2 in an FNR-dependent manner. Presence of nitrate strongly represses ttdA. When glucose is present in addition to L-tartrate, expression of ttdA'-'lacZ is decreased to 24% of the L-tartrate-induced state