General Stability | Organism |
---|---|
100 mM GSH plus 1mM Fe2+ optimal reactivation of inactive enzyme | Pseudomonas putida |
crude cell-extract completely loses activity in open tubes in ice bath for 2-4 h, activity restored after incubation at 25°C for 30 min, extent of activation decreases with each succeeding cycle of inactivation and reactivation, loss of activity after treatment with EDTA or after dialysis | Pseudomonas putida |
Cysteine 100 mM Cysteine plus 1 mM Fe2+ optimal reactivation of inactive enzyme | Pseudomonas putida |
equimolar mixture of 50 mM GSH and 50 mM Cysteine plus 1 mM Fe2+ optimum reactivation of inactive enzyme | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R,S)-tartrate | competitive | Pseudomonas putida | |
CdCl2 | - |
Pseudomonas putida | |
CoCl2 | - |
Pseudomonas putida | |
CuCl2 | - |
Pseudomonas putida | |
cysteine | 35% inhibition at 50 mM | Pseudomonas putida | |
D-malic acid | K-salt of | Pseudomonas putida | |
glutathione | 13% inhibition at 50 mM | Pseudomonas putida | |
HgCl2 | - |
Pseudomonas putida | |
L-Malic acid | K-salt of | Pseudomonas putida | |
L-threonic acid | K-salt of | Pseudomonas putida | |
Tartronic acid | K-salt of | Pseudomonas putida | |
Urea | - |
Pseudomonas putida | |
ZnCl2 | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reducing conditions: 2 KM-values/biphasic curve, presence of air: only one higher KM-value obtained | Pseudomonas putida | |
0.81 | - |
(R,R)-Tartrate | relatively anaerobic conditions | Pseudomonas putida | |
2.1 | - |
(R,R)-Tartrate | both, under anaerobic conditions and in the presence of air | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | addition of either GSH or cysteine plus Fe2+ reactivates inactive enzyme, optimum Fe2+ concentration 1 mM, Fe2+ only required for activation | Pseudomonas putida | |
additional information | requirement for Fe2+ cannot be replaced by Mg2+, Ba2+, Mn2+, Co2+, Ni2+, Zn2+, Cu2+, Al3+, BO32-, MoO42- at concentrations of 0.01 mM and 1 mM | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
sucrose density gradient centrifugation, inactive form. Aggregation of the four smaller units forms the active protein | Pseudomonas putida |
145000 | - |
sucrose density gradient centrifugation, active form. Enzyme exists as an aggregate of smaller protein or proteins, the latter possessing no enzyme activity | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,R)-Tartrate | Pseudomonas putida | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Oxidation Stability | Organism |
---|---|
reactivation of the inactive enzyme is seriously inhibited by exposure to air | Pseudomonas putida |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,R)-Tartrate | - |
Pseudomonas putida | ? | - |
? | |
L-(+)-Tartrate | - |
Pseudomonas putida | Oxaloacetate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000 sucrose density gradient centrifugation, inactive form, activation by aggregation of 4 monomeres | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 9.4 | 50% of maximal activity at pH 7.6 and at pH 9.4 | Pseudomonas putida |