BRENDA - Enzyme Database show
show all sequences of 4.2.1.32

Identification of the L-tartrate dehydratase genes (ttdA and ttdB) of Escherichia coli and evolutioary relationship with the Class I fumarase genes

Reaney, S.K.; Begg, C.; Bungard, S.J.; Guest J.R.; J. Gen. Microbiol. 139, 1523-1530 (1993)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpressed in DH5 alpha (pGS581)
Escherichia coli
General Stability
General Stability
Organism
cell extracts are prepared in extraction buffer and maintain at 0°C under N2 for more than 1 h before assaying for enzyme activity
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.76
-
(R,R)-Tartrate
overexpressed strain DH5alpha(pGS581)
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22641
-
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme
Escherichia coli
32589
-
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme
Escherichia coli
105000
-
gel filtration
Escherichia coli
110500
-
DNA-derived values of TtdA and TtdB
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R,R)-Tartrate
Escherichia coli
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
K12 wild-type W3110; overexpressed in DH5alpha(pGS581)
-
Oxidation Stability
Oxidation Stability
Organism
oxygen-labile
Escherichia coli
Storage Stability
Storage Stability
Organism
-20°C, cell-extracts in air 90% loss of activity in less than 10 h
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R,R)-Tartrate
-
5656
Escherichia coli
?
-
-
-
-
(R,S)-Tartrate
in the presence of glycerol and (R,S)-tartrate under anaerobic conditions
5656
Escherichia coli
Oxaloacetate + H2O
-
5656
Escherichia coli
-
L-(+)-Tartrate
in the presence of glycerol (R,R)-tartrate can be used as reducible substrate for supporting anaerobic growth
5656
Escherichia coli
Oxaloacetate + H2O
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpressed in DH5 alpha (pGS581)
Escherichia coli
General Stability (protein specific)
General Stability
Organism
cell extracts are prepared in extraction buffer and maintain at 0°C under N2 for more than 1 h before assaying for enzyme activity
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.76
-
(R,R)-Tartrate
overexpressed strain DH5alpha(pGS581)
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22641
-
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme
Escherichia coli
32589
-
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme
Escherichia coli
105000
-
gel filtration
Escherichia coli
110500
-
DNA-derived values of TtdA and TtdB
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R,R)-Tartrate
Escherichia coli
-
?
-
-
-
Oxidation Stability (protein specific)
Oxidation Stability
Organism
oxygen-labile
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, cell-extracts in air 90% loss of activity in less than 10 h
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R,R)-Tartrate
-
5656
Escherichia coli
?
-
-
-
-
(R,S)-Tartrate
in the presence of glycerol and (R,S)-tartrate under anaerobic conditions
5656
Escherichia coli
Oxaloacetate + H2O
-
5656
Escherichia coli
-
L-(+)-Tartrate
in the presence of glycerol (R,R)-tartrate can be used as reducible substrate for supporting anaerobic growth
5656
Escherichia coli
Oxaloacetate + H2O
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme
Escherichia coli
Other publictions for EC 4.2.1.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
705573
Kim
Regulation of tartrate metabol ...
Escherichia coli
Microbiology
155
3632-3640
2009
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1
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2
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2
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677853
Kim
Anaerobic growth of Escherichi ...
Escherichia coli
Arch. Microbiol.
188
583-589
2007
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1
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3
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1
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1
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5656
Reaney
Identification of the L-tartra ...
Escherichia coli
J. Gen. Microbiol.
139
1523-1530
1993
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-
1
-
-
1
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1
-
-
4
1
-
3
1
-
-
-
-
-
-
1
3
1
-
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1
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1
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1
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4
1
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1
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1
3
1
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5652
Furuyoshi
-
Occurrence of a New Enzyme, me ...
Pseudomonas putida
Agric. Biol. Chem.
51
1495-1499
1987
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-
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1
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1
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1
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5654
Kelly
-
L-(+)-Tartrate dehydratase fro ...
Pseudomonas putida
FEBS Lett.
202
274-276
1986
-
-
-
-
-
2
1
-
-
1
3
-
-
1
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-
1
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1
-
1
1
1
-
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2
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1
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1
3
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1
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1
-
1
1
1
-
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-
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-
5655
Schink
-
Fermentation of tartrate enant ...
Acetivibrio sp., Bacteria, Bacteroides sp., Ilyobacter sp., Ilyobacter tartaricus, Trichococcus pasteurii
Arch. Microbiol.
139
409-414
1984
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6
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6
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12
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6
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12
-
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5657
Yashphe
The loss of meso-tartrate dehy ...
Pseudomonas sp.
Biochim. Biophys. Acta
146
560-576
1967
-
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-
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1
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1
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1
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5651
Hurlbert
-
L-(+)-Tartaric acid dehydrase ...
Pseudomonas putida
Methods Enzymol.
9
680-682
1966
-
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-
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-
1
2
3
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1
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1
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1
2
1
1
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1
1
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1
2
1
1
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1
1
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5653
Hurlbert
Tartaric acid metabolism ...
Pseudomonas putida
J. Biol. Chem.
240
2772-2777
1965
-
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4
13
3
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2
2
1
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1
1
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1
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2
1
1
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1
1
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4
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13
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3
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2
2
1
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1
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1
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2
1
1
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1
1
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