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The taxonomic range for the selected organisms is: Pseudomonas putida
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
phenylserine aldolase,
more
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5-dehydro-2-deoxy-D-gluconate-6-phosphate malonate-semialdehyde-lyase
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aldolase, phenylserine
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-
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L-threo-3-phenylserine benzaldehyde-lyase (glycine-forming)
A pyridoxal-phosphate protein.
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glycine + benzaldehyde
L-threo-3-phenylserine + L-erythro-3-phenylserine
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r
L-allo-Thr
glycine + acetaldehyde
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-
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?
L-erythro-3-phenylserine
glycine + benzaldehyde
no activity with D-erythro-3-phenylserine
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r
L-Thr
glycine + acetaldehyde
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?
L-threo-3-phenylserine
glycine + benzaldehyde
no activity with D-threo-3-phenylserine
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r
additional information
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additional information
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inducible enzyme
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?
additional information
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inducible enzyme
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additional information
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no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine
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?
additional information
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no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine
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?
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additional information
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additional information
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inducible enzyme
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?
additional information
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inducible enzyme
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?
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pyridoxal 5'-phosphate
0.7 mol of pyridoxal 5'-phosphate per mol of subunit, K213 of the enzyme probably forms a Schiff base with pyridoxal 5'-phosphate
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3-(ethylamino)phenol
competitive against DL-threo-3-phenylserine
D-cycloserine
1 mM, 98% inhibition
DL-3-hydroxy-n-butyrate
20 mM, 30% inhibition
DL-3-hydroxynorvaline
20 mM, 49% inhibition
DL-3-hydroxyphenylethylamine
20 mM, 79% inhibition
hydroxylamine
1 mM, 97% inhibition
L-3-phenyllactate
10 mM, 39% inhibition
phenylhydrazine
1 mM, 52% inhibition
Semicarbazide
1 mM, 97% inhibition
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22
L-allo-Thr
pH 8.5, 30°C
4.6
L-erythro-3-phenylserine
pH 8.5, 30°C
1.3
L-threo-3-phenylserine
pH 8.5, 30°C
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1300
L-allo-Thr
pH 8.5, 30°C
7900
L-erythro-3-phenylserine
pH 8.5, 30°C
2300
L-threo-3-phenylserine
pH 8.5, 30°C
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4.1
3-(ethylamino)phenol
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300
cleavage of L-threo-3-phenylserine
4.3
reaction with glycine and benzaldehyde
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7.5
reaction with glycine and benzyldehyde
8.5
reaction with L-threo-3-phenylserine
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strain 24-1
SwissProt
brenda
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Q59IT3_PSEPU
357
0
38317
TrEMBL
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37400
6 * 37400, calculation from nucleotide sequence
38000
6 * 38000, SDS-PAGE
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hexamer
6 * 38000, SDS-PAGE
hexamer
6 * 37400, calculation from nucleotide sequence
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K213Q
loss of activity, disappearance of absorption maximum at 420 nm
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6.5 - 9.5
30°C, 10 min, stable
663708
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-20°C, pH 7.2, 10 mM TES buffer, 0.01% 2-mercaptoethanol, 0.05 mM pyridoxal 5'-phosphate, 30% glycerol, stable for several months
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overexpression in Escherichia coli
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Misono, H.; Maeda, H.; Tuda, K.; Ueshima, S.; Miyazaki, N.; Nagata, S.
Characterization of an inducible phenylserine aldolase from Pseudomonas putida 24-1
Appl. Environ. Microbiol.
71
4602-4609
2005
Pseudomonas putida (Q59IT3), Pseudomonas putida, Pseudomonas putida 24. Jan (Q59IT3)
brenda