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Literature summary for 4.1.2.26 extracted from

  • Misono, H.; Maeda, H.; Tuda, K.; Ueshima, S.; Miyazaki, N.; Nagata, S.
    Characterization of an inducible phenylserine aldolase from Pseudomonas putida 24-1 (2005), Appl. Environ. Microbiol., 71, 4602-4609.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
K213Q loss of activity, disappearance of absorption maximum at 420 nm Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
3-(ethylamino)phenol competitive against DL-threo-3-phenylserine Pseudomonas putida
D-cycloserine 1 mM, 98% inhibition Pseudomonas putida
DL-3-hydroxy-n-butyrate 20 mM, 30% inhibition Pseudomonas putida
DL-3-hydroxynorvaline 20 mM, 49% inhibition Pseudomonas putida
DL-3-hydroxyphenylethylamine 20 mM, 79% inhibition Pseudomonas putida
hydroxylamine 1 mM, 97% inhibition Pseudomonas putida
L-3-phenyllactate 10 mM, 39% inhibition Pseudomonas putida
phenylhydrazine 1 mM, 52% inhibition Pseudomonas putida
Semicarbazide 1 mM, 97% inhibition Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
L-threo-3-phenylserine pH 8.5, 30┬░C Pseudomonas putida
4.6
-
L-erythro-3-phenylserine pH 8.5, 30┬░C Pseudomonas putida
22
-
L-allo-Thr pH 8.5, 30┬░C Pseudomonas putida
29
-
L-Thr pH 8.5, 30┬░C Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37400
-
6 * 37400, calculation from nucleotide sequence Pseudomonas putida
38000
-
6 * 38000, SDS-PAGE Pseudomonas putida
210000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas putida inducible enzyme ?
-
?
additional information Pseudomonas putida 24. Jan inducible enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q59IT3 strain 24-1
-
Pseudomonas putida 24. Jan Q59IT3 strain 24-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
4.3
-
reaction with glycine and benzaldehyde Pseudomonas putida
300
-
cleavage of L-threo-3-phenylserine Pseudomonas putida

Storage Stability

Storage Stability Organism
-20┬░C, pH 7.2, 10 mM TES buffer, 0.01% 2-mercaptoethanol, 0.05 mM pyridoxal 5'-phosphate, 30% glycerol, stable for several months Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + benzaldehyde
-
Pseudomonas putida L-threo-3-phenylserine + L-erythro-3-phenylserine
-
r
glycine + benzaldehyde
-
Pseudomonas putida 24. Jan L-threo-3-phenylserine + L-erythro-3-phenylserine
-
r
L-allo-Thr
-
Pseudomonas putida glycine + acetaldehyde
-
?
L-erythro-3-phenylserine no activity with D-erythro-3-phenylserine Pseudomonas putida glycine + benzaldehyde
-
r
L-Thr
-
Pseudomonas putida glycine + acetaldehyde
-
?
L-Thr
-
Pseudomonas putida 24. Jan glycine + acetaldehyde
-
?
L-threo-3-phenylserine no activity with D-threo-3-phenylserine Pseudomonas putida glycine + benzaldehyde
-
r
L-threo-3-phenylserine no activity with D-threo-3-phenylserine Pseudomonas putida 24. Jan glycine + benzaldehyde
-
r
additional information inducible enzyme Pseudomonas putida ?
-
?
additional information no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine Pseudomonas putida ?
-
?
additional information inducible enzyme Pseudomonas putida 24. Jan ?
-
?
additional information no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine Pseudomonas putida 24. Jan ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 38000, SDS-PAGE Pseudomonas putida
hexamer 6 * 37400, calculation from nucleotide sequence Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
580
-
L-Thr pH 8.5, 30┬░C Pseudomonas putida
1300
-
L-allo-Thr pH 8.5, 30┬░C Pseudomonas putida
2300
-
L-threo-3-phenylserine pH 8.5, 30┬░C Pseudomonas putida
7900
-
L-erythro-3-phenylserine pH 8.5, 30┬░C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
reaction with glycine and benzyldehyde Pseudomonas putida
8.5
-
reaction with L-threo-3-phenylserine Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9.5 30┬░C, 10 min, stable Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 0.7 mol of pyridoxal 5'-phosphate per mol of subunit, K213 of the enzyme probably forms a Schiff base with pyridoxal 5'-phosphate Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1
-
3-(ethylamino)phenol
-
Pseudomonas putida