Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.8.1.5 - haloalkane dehalogenase and Organism(s) Psychrobacter cryohalolentis and UniProt Accession Q1QBB9

for references in articles please use BRENDA:EC3.8.1.5
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.8 Acting on halide bonds
             3.8.1 In carbon-halide compounds
                3.8.1.5 haloalkane dehalogenase
IUBMB Comments
Acts on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Psychrobacter cryohalolentis
UNIPROT: Q1QBB9
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Psychrobacter cryohalolentis
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
haloalkane dehalogenase, haloalkane dehalogenase linb, dhaa31, hld-i, rv2579, ehld-c, linbut, ylehd, 1-chlorohexane halidohydrolase, linbmi, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1-chlorohexane halidohydrolase
-
-
-
-
1-haloalkane dehalogenase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of C-halide
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
1-haloalkane halidohydrolase
Acts on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds.
CAS REGISTRY NUMBER
COMMENTARY hide
95990-29-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1-bromomethyl)cyclohexane + H2O
?
show the reaction diagram
-
low activity
-
-
?
1,2-dibromopropane + H2O
2-bromo-1-propanol + bromide
show the reaction diagram
-
low activity
-
-
?
1,3-dibromopropane + H2O
3-bromo-1-propanol + bromide
show the reaction diagram
-
high activity
-
-
?
1,3-diiodopropane + H2O
3-iodo-1-propanol + iodide
show the reaction diagram
-
-
-
-
?
1,5-dichloropentane + H2O
5-chloro-1-pentanol + chloride
show the reaction diagram
-
low activity
-
-
?
1-bromo-2-chloroethane + H2O
2-chloroethanol + bromide
show the reaction diagram
-
low activity
-
-
?
1-bromo-3-chloropropane + H2O
?
show the reaction diagram
-
-
-
-
?
1-bromobutane + H2O
1-butanol + bromide
show the reaction diagram
-
high activity
-
-
?
1-bromohexane + H2O
1-hexanol + bromide
show the reaction diagram
-
high activity
-
-
?
1-chlorohexane + H2O
n-hexanol + chloride
show the reaction diagram
-
low activity
-
-
?
1-iodobutane + H2O
n-butanol + iodide
show the reaction diagram
-
-
-
-
?
1-iodohexane + H2O
n-hexanol + iodide
show the reaction diagram
-
-
-
-
?
1-iodopropane + H2O
n-propanol + iodide
show the reaction diagram
-
-
-
-
?
2-iodobutane + H2O
2-butanol + iodide
show the reaction diagram
-
-
-
-
?
3-chloro-2-methylpropene + H2O
?
show the reaction diagram
-
low activity
-
-
?
4-bromobutyronitrile + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
substrate specificity profiles of DpcA, DpcA prefers brominated or iodinated substrates over chlorinated ones, overview
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.77
1,3-dibromopropane
-
pH 8.7, 37°C
3.83
1-bromobutane
-
pH 8.7, 37°C
additional information
additional information
-
steady-state kinetic analysis with substrates 1-bromobutane and 1,3-dibromopropane, positive cooperative substrate binding, overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.72
1,3-dibromopropane
-
pH 8.7, 37°C
1.1
1-bromobutane
-
pH 8.7, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.7
-
with substrate 1-bromobutane
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 9.6
-
DpcA loses all activity below pH 6.4 and above pH 9.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 50
-
with substrate 1-bromobutane
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs
evolution
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
sequence-based screening and enzyme identification, sequence comparisons, folding and secondary structure of DpcA, overview
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure determination and analysis, PDB IDs 4BRZ and 4C6H
purified enzyme free or in complex with ligands 1-bromohexane and 1,2-dichloroethane, sitting or haning drop vapour diffusion technique, mixing 0.002 ml protein solution and 0.001 ml precipitant solution plus 300-600 nl 0.1 M L-proline, optimization of crystallization conditions, enzyme DpcA with 10% v/v 1-bromohexane in a solution of 0.1 M glycine buffer pH 8.6, mixing protein, precipitant solution and 1-bromohexane in a 1:1:1.3 ml ratio and adding 0.00033 ml 0.1 M l-proline as an additive, DpcA complex with 1,2-dichloroethane obtained by soaking experiments after the addition of 0.05 ml 1,2-dichloroethane to the reservoir well containing 0.8 ml of the precipitating solution with the crystals and incubation for 7 days, 19°C, X-ray diffraction structure determination and analysis at 1.05-1.65 A resolution
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminally His-tagged enzyme is expressed in Escherichia coli BL21(DE3) ArcticExpress cells
phylogenetic analysis and tree
gene dpcA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Drienovska, I.; Chovancova, E.; Koudelakova, T.; Damborsky, J.; Chaloupkova, R.
Biochemical characterization of a novel haloalkane dehalogenase from a cold-adapted bacterium
Appl. Environ. Microbiol.
78
4995-4998
2012
Psychrobacter cryohalolentis, Psychrobacter cryohalolentis K5
Manually annotated by BRENDA team
Tratsiak, K.; Degtjarik, O.; Drienovska, I.; Chrast, L.; Rezacova, P.; Kuty, M.; Chaloupkova, R.; Damborsky, J.; Kuta Smatanova, I.
Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17
Acta Crystallogr. Sect. F
69
683-688
2013
Marinobacter sp., Psychrobacter cryohalolentis, Psychrobacter cryohalolentis K5
Manually annotated by BRENDA team
Nagata, Y.; Ohtsubo, Y.; Tsuda, M.
Properties and biotechnological applications of natural and engineered haloalkane dehalogenases
Appl. Microbiol. Biotechnol.
99
9865-9881
2015
Agrobacterium tumefaciens (E2RV69), Agrobacterium tumefaciens C58 / ATCC 33970 (E2RV69), Alcanivorax dieselolei, Alcanivorax dieselolei B-5, Arthrobacter sp., Bradyrhizobium elkanii (E2RV62), Bradyrhizobium elkanii USDA94 (E2RV62), Bradyrhizobium japonicum (P59337), Bradyrhizobium japonicum USDA 110 (P59337), Corynebacterium sp., Marinobacter sp. (A3JB27), Mesorhizobium loti (Q98C03), Mesorhizobium loti MAFF303099 (Q98C03), Mycobacterium avium (Q93K00), Mycobacterium avium N85 (Q93K00), Mycobacterium sp. (Q9ZER0), Mycobacterium sp. GP1 (Q9ZER0), Mycobacterium tuberculosis (P9WMR9), Mycobacterium tuberculosis H37Rv (P9WMR9), Mycobacterium tuberculosis variant bovis (A4Q9R7), Mycobacterium tuberculosis variant bovis (Q6EUU8), Mycobacterium tuberculosis variant bovis (Q9XB14), Mycobacterium tuberculosis variant bovis 5033/66 (A4Q9R7), Mycobacterium tuberculosis variant bovis 5033/66 (Q6EUU8), Mycobacterium tuberculosis variant bovis 5033/66 (Q9XB14), Plesiocystis pacifica (A6G7B1), Psychrobacter cryohalolentis (Q1QBB9), Psychrobacter cryohalolentis K5 (Q1QBB9), Rhodobacteraceae bacterium UDC319 (A0A023I2Y1), Rhodococcus rhodochrous (P0A3G2), Rhodococcus rhodochrous NCIMB 13064 (P0A3G2), Rhodococcus sp., Rhodopirellula baltica (G3XCP3), Rhodopirellula baltica SH1 (G3XCP3), Sphingobium indicum, Sphingobium indicum (P51698), Sphingobium indicum B90A, Sphingobium indicum UT26 (P51698), Sphingobium sp. (A4PEU6), Sphingobium sp. MI1205 (A4PEU6), Sphingomonas sp., Sphingomonas sp. BHC-A, Strongylocentrotus purpuratus, Xanthobacter autotrophicus (P22643), Xanthobacter autotrophicus GJ10 (P22643)
Manually annotated by BRENDA team
Tratsiak, K.; Prudnikova, T.; Drienovska, I.; Damborsky, J.; Brynda, J.; Pachl, P.; Kuty, M.; Chaloupkova, R.; Rezacova, P.; Kuta Smatanova, I.
Crystal structure of the cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5
Acta Crystallogr. Sect. F
75
324-331
2019
Psychrobacter cryohalolentis (Q1QBB9), Psychrobacter cryohalolentis K5 (Q1QBB9), Psychrobacter cryohalolentis K5
Manually annotated by BRENDA team