EC Number |
---|
3.8.1.5 | - |
3.8.1.5 | 12 mg/ml purified recombinant His-tagged enzyme, effective crystallization conditions are not same as for the nontagged enzyme, hanging drop vapour diffusion method, 200 mM MgCl2, 100 mM Tris-HCl, pH 8.5, 20% w/v PEG 4000, equal volumes of 0.001 ml of protein and well solution are mixed on siliconized cover slips, 3 days, crystals are used for seeding, another 3 days, X-ray diffraction structure determination and analysis at 0.95 A resolution, molecular dynamics simulation |
3.8.1.5 | cocrystallization with sodium iodide, structure at pH 5.5, pH 7.0 and with bound iodide, alpha/beta hydrolase structure |
3.8.1.5 | comparison of crystal structures of haloalkane dehalogenases by homology models |
3.8.1.5 | crystal structure determination and analysis |
3.8.1.5 | crystal structure determination and analysis, PDB ID 1CIJ |
3.8.1.5 | crystal structure determination and analysis, PDB ID 1CQW |
3.8.1.5 | crystal structure determination and analysis, PDB ID 1CV2/2BFN |
3.8.1.5 | crystal structure determination and analysis, PDB ID 2QVB |
3.8.1.5 | crystal structure determination and analysis, PDB ID 2XT0 |