HOME
login
history
all enzymes
BRENDA home
History
Information on EC 3.8.1.5 - haloalkane dehalogenase
for references in articles please use BRENDA:EC3.8.1.5Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.8.1.5 haloalkane dehalogenaseIUBMB Comments
Acts on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds.
Specify your search results
Word Map
- 3.8.1.5
- xanthobacter
-
dehalogenation
- autotrophicus
- 1,2-dichloroethane
- halide
- 1,2-dibromoethane
-
carbon-halogen
- sphingomonas
- paucimobilis
- 1,2,3-trichloropropane
- sphingobium
- hexachlorocyclohexane
- rhodochrous
- synthesis
- environmental protection
-
alkyl-enzyme
- 1-chlorobutane
- 2-chloroethanol
-
haloacid
-
ncimb
- gamma-hexachlorocyclohexane
- agriculture
-
halide-binding
- epichlorohydrine
- biotechnology
-
chloroalkane
- haloalcohols
-
alpha/beta-hydrolase
- dehydrochlorinase
- degradation
- industry
The enzyme appears in viruses and cellular organisms
Synonyms
haloalkane dehalogenase, dhaa31, haloalkane dehalogenase linb, hld-i, rv2579, ehld-c, 1-chlorohexane halidohydrolase, linbut, ylehd, linbmi, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1,3,4,6,-tetrachloro-1,4-cyclohexadiene halidohydrolase
DadB
DatA
DbeA
DbjA
DccA
DhaA
DhaA31
DhaB
DhaC
DhAf
DhlA
DhmA
dmbA
DmbB
DmbC
dmlA
DmmA
DmrA
DmrB
DmtA
DmxA
DpcA
DrbA
haloalkane dehalogenase
haloalkane dehalogenase LinB
HanR
HLD
HLD-I
LinB
LinBMI
LinBUT
protein XP_504164
Rv2579
Ylehd
DhaA
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1-haloalkane + H2O = a primary alcohol + halide
-
-
-
-
1-haloalkane + H2O = a primary alcohol + halide
GJ10, three-step reaction mechanism with covalent alkyl-enzyme ester intermediate
1-haloalkane + H2O = a primary alcohol + halide
GJ10, four-step reaction mechanism with covalent ester intermediate
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
-
1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
-
1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
1-haloalkane + H2O = a primary alcohol + halide
GJ10, detailed reaction mechanism and energetics calculated from computational model
1-haloalkane + H2O = a primary alcohol + halide
two-step mechanism involving an ester intermediate covalently bound at Asp124, His 289 is important for hydrolysis
-
1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Asn38 is involved, SN2 reaction mechanism, modeling, overview
-
1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Asn41 is involved, SN2 reaction mechanism, modeling, overview
-
1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Glu56 is involved, SN2 reaction mechanism, modeling, overview
1-haloalkane + H2O = a primary alcohol + halide
acts on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds
-
1-haloalkane + H2O = a primary alcohol + halide
catalytic nucleophile Asp108, which is the most disordered catalytic residue, catalytic base His272, structure-based reaction mechanism, active site structure, overview
-
1-haloalkane + H2O = a primary alcohol + halide
low activity with small, chlorinated alkanes, importance of active site water molecules and reaction products in molecular docking
-
1-haloalkane + H2O = a primary alcohol + halide
substrate specificity is influenced by the size and shape of their entrance tunnel, surface residue Leu177 is involved and located at the tunnel opening
-
1-haloalkane + H2O = a primary alcohol + halide
activation energy of reaction 59.5 kJ/mol
-
1-haloalkane + H2O = a primary alcohol + halide
catalytic triad consists of D123, H279, D250, with a first halide-stabilizing W124 and a secong stabilizing residue W164. Two-step reaction mechanism
1-haloalkane + H2O = a primary alcohol + halide
H272 singly protonated at Ndelta1 and D108 in conformation A give the most exothermic reactionwith DeltaH of -22 kcal/mol
-
1-haloalkane + H2O = a primary alcohol + halide
hydrogen bond interactions between substrate and the environment are significantly different in aqueous solution and in the enzyme. Structure of the enzyme active site provides a more adequate interaction pattern for the reaction process
1-haloalkane + H2O = a primary alcohol + halide
Asp103, Glu127 and His280 are involved in the catalytic reaction, and two H-bond donating residues, Asn38 and Trp104, are involved in stabilization of a halogen group of the substrate
1-haloalkane + H2O = a primary alcohol + halide
reaction mechanism, overview. The bromide release is the rate-limiting step of the catalytic cycle, overview
-
1-haloalkane + H2O = a primary alcohol + halide
structural basis for its reaction mechanism
1-haloalkane + H2O = a primary alcohol + halide
Asp103, Glu127 and His280 are involved in the catalytic reaction, and two H-bond donating residues, Asn38 and Trp104, are involved in stabilization of a halogen group of the substrate
-
1-haloalkane + H2O = a primary alcohol + halide
catalytic nucleophile Asp108, which is the most disordered catalytic residue, catalytic base His272, structure-based reaction mechanism, active site structure, overview
-
1-haloalkane + H2O = a primary alcohol + halide
H272 singly protonated at Ndelta1 and D108 in conformation A give the most exothermic reactionwith DeltaH of -22 kcal/mol
-
1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
-
1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Asn38 is involved, SN2 reaction mechanism, modeling, overview
-
1-haloalkane + H2O = a primary alcohol + halide
low activity with small, chlorinated alkanes, importance of active site water molecules and reaction products in molecular docking
-
1-haloalkane + H2O = a primary alcohol + halide
substrate specificity is influenced by the size and shape of their entrance tunnel, surface residue Leu177 is involved and located at the tunnel opening
-
1-haloalkane + H2O = a primary alcohol + halide
catalytic triad consists of D123, H279, D250, with a first halide-stabilizing W124 and a secong stabilizing residue W164. Two-step reaction mechanism
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
structural basis for its reaction mechanism
Agrobacterium tumefaciens C58 / ATCC 33970
-
1-haloalkane + H2O = a primary alcohol + halide
reaction mechanism, overview. The bromide release is the rate-limiting step of the catalytic cycle, overview
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
two-step mechanism involving an ester intermediate covalently bound at Asp124, His 289 is important for hydrolysis
-
1-haloalkane + H2O = a primary alcohol + halide
GJ10, four-step reaction mechanism with covalent ester intermediate
-
1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
-
1-haloalkane + H2O = a primary alcohol + halide
GJ10, three-step reaction mechanism with covalent alkyl-enzyme ester intermediate
-
1-haloalkane + H2O = a primary alcohol + halide
GJ10, detailed reaction mechanism and energetics calculated from computational model
-
1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Glu56 is involved, SN2 reaction mechanism, modeling, overview
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
activation energy of reaction 59.5 kJ/mol
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C-halide hydrolysis
hydrolysis of C-halide
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
KEGG
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
1-haloalkane halidohydrolase
Acts on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
95990-29-7
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1S,3S)-2,3,4,5,6-pentachlorocyclohexanol + H2O
cis-2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride
(RS)-3-chloropropane-1,2-diol + NAD+
(R)-3-chloropropane-1,2-diol + 2-oxo-propionaldehyde + CH3COOH + Cl- + HCOOH + NADH
-
-
-
?
1,2,3-tribromopropane + H2O
2,3-dibromopropanol + chloride
-
-
-
?
1,2,3-trichloropropane + H2O
2,3-dichloropropan-1-ol
-
prefered formation of R-enantiomer due to a specific interaction of substrate with residue N41
?
1,2,3-trichloropropane + H2O
2,3-dichloropropanol + chloride
-
-
?
1,2,3-trichloropropane + H2O
2,3-trichloropropan-1-ol + chloride
1,2,3-trichloropropane is a persistent anthropogenic toxic pollutant that can be converted to the less toxic 2,3-dichloropropan-1-ol
-
?
1,2,3-trichloropropene + H2O
2,3-dichloropropenol + chloride
-
-
?
1,2-dibromo-3-chloropropane
2-bromo-3-chloro-1-propanol + bromide
about 35% of the activity compared to 4-bromobutyronitrile
-
?
1,2-dibromohexane + H2O
2-bromohexanol + bromide
-
-
?
1,2-dichlorobutane + H2O
?
-
7.8% of the activity with 4-chlorobutanol
-
?
1,2-dichloroethane + H2O
2-bromo-1-ethanol + bromide
-
less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O
2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
1,3,4,6-tetrachloro-1,4-cyclohexadiene + H2O
2,4,5-trichloro-2,5-cyclohexadiene-1-ol + chloride
-
-
?
1,3-dibromobutane + H2O
3-bromo-1-propanol + bromide
about 20% of the activity compared to 4-bromobutyronitrile
-
?
1,3-dibromopropane + H2O
3-bromo-1-propanol bromide
-
about 40% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1,3-dichlorobutane + H2O
?
-
12% of the activity with 4-chlorobutanol
-
?
1,3-dichloropropane + H2O
3-chloropropan-1-ol + chloride
-
-
?
1,3-dichloropropane + H2O
3-iodo-1-propanol + iodide
-
about 10% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1,4-dichlorobutane + H2O
?
-
27% of the activity with 4-chlorobutanol
-
?
1,5-dichloropentane + H2O
?
-
20% of the activity with 4-chlorobutanol
-
?
1,6-dibromohexane + H2O
6-bromohexane + bromide
-
low activity
-
?
1,6-dichlorohexane + H2O
6-chlorohexane + bromide
-
low activity
-
?
1,6-dichlorohexane + H2O
?
-
32% of the activity with 4-chlorobutanol
-
?
1,6-diiodohexane + H2O
6-iodohexane + iodide
-
low activity
-
?
1,7-dichloroheptane + H2O
?
-
20% of the activity with 4-chlorobutanol
-
?
1,8-dichlorooctane + H2O
?
-
32% of the activity with 4-chlorobutanol
-
?
1,9-dichlorononane + H2O
?
-
14% of the activity with 4-chlorobutanol
-
?
1-bromo-2-chloroethane
2-chloro-1-ethanol + bromide
about 50% of the activity compared to 4-bromobutyronitrile
-
?
1-bromo-2-chloroethane + H2O
1-bromo-2-ethanol + chloride
-
low activity
-
?
1-bromo-2-chloroethane + H2O
?
-
76% of the activity with 1,2-dichloroethane
-
?
1-chloro-2-(2-chloroethoxy)ethane + H2O
2-(2-chloroethoxy)ethanol + chloride
-
-
?
1-chloro-2-bromoethane + H2O
2-bromoethanol + chloride
-
-
-
?
1-chloro-2-fluoroethane + H2O
?
-
no release of fluoride, 25% of the activity with 1,2-dichloroethane
-
?
1-chloro-4-methylbutane + H2O
pentanol + chloride
-
42% of the activity with 4-chlorobutanol
-
?
1-chlorononane + H2O
nonanol + chloride
-
13% of the activity with 4-chlorobutanol
-
?
2,3,4,5,6-pentachlorocyclohexanol + H2O
2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride
2-bromo-1-chloropropane + H2O
2-bromo-1-propanol + chloride
-
about 50% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
2-bromo-1-chloropropane + H2O
2-bromo-1propanol + chloride
-
about 60% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
2-bromoacetamide + H2O
acetamide + bromide
poor substrate
-
?
2-chloroacetamide + H2O
acetamide + chloride
poor substrate
-
?
2-chloroethanol + H2O
ethanol + chloride
very poor substrate
-
?
3,4,5,6-tetrachloro-2-cyclohexene-1-ol + H2O
2,5,6-trichloro-2-cyclohexene-1,4-diol + chloride
3-chloro-2-(chloromethyl)-1-propene + H2O
2-chloromethyl-2-propen-1-ol + Cl-
-
-
-
?
3-chloro-2-methylpropene + H2O
2-methylpropene + chloride
-
-
-
?
3-chloropropene + H2O
propenol + chloride
-
45% of the activity with 1,2-dichloroethane
-
?
4-chlorobutanol + H2O
butane-1,4-diol + chloride
-
highest activity
-
?
bromochloromethane + H2O
?
-
99% of the activity with 1,2-dichloroethane
-
?
chlorocyclopentane + H2O
cyclopentanol + choride
-
about 10% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
chlorocyclopentane + H2O
hydroxycyclopentane + chloride
-
-
-
?
delta-hexachlorocyclohexane + H2O
(1S,3S)-2,3,4,5,6-pentachlorocyclohexanol + chloride
-
-
-
?
delta-pentachlorocyclohexene + H2O
3,4,5,6-tetrachloro-2-cyclohexene-1-ol + chloride
-
-
-
?
dichloromethane + H2O
chloromethanol + chloride
poor substrate
-
?
epsilon-hexachlorocyclohexane + H2O
pentachlorocyclohexanol + tetrachlorocyclohexane-1,4-diol + chloride
gamma-pentachlorocyclohexene + H2O
3,4,5,6-tetrachloro-2-cyclohexene-1-ol + 2,5,6-trichloro-2-cyclohexene-1,4-diol + chloride
-
-
-
?
pentachlorocyclohexanol + H2O
tetrachlorocyclohexanediol + chloride
-
-
-
?
(1-bromomethyl)cyclohexane + H2O
?
-
2.5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
(1-bromomethyl)cyclohexane + H2O
?
-
less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
(1S,3S)-2,3,4,5,6-pentachlorocyclohexanol + H2O
cis-2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride
-
-
-
?
(1S,3S)-2,3,4,5,6-pentachlorocyclohexanol + H2O
cis-2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride
-
-
-
?
(bromomethyl)cyclohexane + H2O
cyclohexylmethanol + bromide
-
-
?
(bromomethyl)cyclohexane + H2O
cyclohexylmethanol + bromide
-
-
-
?
1,1,2,3,4,5,6-heptachlorocyclohexane + H2O
2,3,4,4,5,6-hexachlorocyclohexanol + chloride
-
-
-
?
1,1,2,3,4,5,6-heptachlorocyclohexane + H2O
2,3,4,4,5,6-hexachlorocyclohexanol + chloride
-
-
-
?
1,2,3-tribromopropane + H2O
2,3-dibromo-1-propanol + bromide
about 10% of the activity compared to 4-bromobutyronitrile
-
?
1,2,3-tribromopropane + H2O
2,3-dibromo-1-propanol + bromide
-
-
-
?
1,2,3-tribromopropane + H2O
2,3-dibromo-1-propanol + bromide
-
-
-
?
1,2,3-tribromopropane + H2O
2,3-dibromo-1-propanol + bromide
-
about 50% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1,2,3-tribromopropane + H2O
2,3-dibromopropanol + bromide
high activity
-
?
1,2,3-tribromopropane + H2O
2,3-dibromopropanol + bromide
-
-
?
1,2,3-tribromopropane + H2O
2,3-dibromopropanol + bromide
-
-
?
1,2,3-tribromopropane + H2O
2,3-dibromopropanol + bromide
low activity
-
?
1,2,3-trichloropropane + H2O
(RS)-2,3-dichloropropan-1-ol + chloride
-
-
-
?
1,2,3-trichloropropane + H2O
(RS)-2,3-dichloropropan-1-ol + chloride
-
structural model of DhaA31 with TCP docked in the active site, comparison with DhaA31 mutant enzyme docking models, overview. The pentad is made up of an Asp-His-Asp catalytic triad and a Trp-Trp or Trp-Asn diad for halide binding. An aspartate residue acts as the nucleophile to displace a halide ion from the substrate. By hydrolysis of the resulting covalent alkyl-enzyme intermediate, alcohol is released
-
?
1,2,3-trichloropropane + H2O
2,3-dichloro-1-propanol + chloride
-
low activity
-
?
1,2,3-trichloropropane + H2O
2,3-dichloro-1-propanol + chloride
-
low activity
-
?
1,2,3-trichloropropane + H2O
2,3-dichloro-1-propanol + chloride
-
-
-
?
1,2,3-trichloropropane + H2O
2,3-dichloro-1-propanol + chloride
-
-
-
?
1,2,3-trichloropropane + H2O
2,3-dichloropropan-1-ol + chloride
-
substrate, but not the product, adsorbs with high affinity onto the polyethylenimine impregnated gamma-alumina support used for enzyme immobilization
-
?
1,2,3-trichloropropane + H2O
2,3-dichloropropan-1-ol + chloride
-
-
?
1,2,3-trichloropropane + H2O
2,3-dichlorpropane-1-ol + chloride
-
-
ir
1,2,3-trichloropropane + H2O
2,3-dichlorpropane-1-ol + chloride
-
-
ir
1,2,3-trichloropropane + H2O
2,3-dichlorpropane-1-ol + chloride
-
-
ir
1,2,3-trichloropropane + H2O
?
-
substrate of DhaA31, a mutant of DhaA with enhanced catalytic activity for 1,2,3-trichloropropane
-
?
1,2,3-trichloropropane + H2O
?
-
substrate of DhaA31, a mutant of DhaA with enhanced catalytic activity for 1,2,3-trichloropropane
-
?
1,2-dibromo-3-chloropropane + H2O
2-bromo-3-chloro-1-propanol + bromide
-
about 40% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1,2-dibromo-3-chloropropane + H2O
2-bromo-3-chloro-1-propanol + bromide
-
about 5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
1,2-dibromo-3-chloropropane + H2O
2-bromo-3-chloropropanol + bromide
high activity
-
?
1,2-dibromo-3-chloropropane + H2O
2-bromo-3-chloropropanol + bromide
-
-
?
1,2-dibromo-3-chloropropane + H2O
2-bromo-3-chloropropanol + bromide
-
-
-
?
1,2-dibromoethane + H2O
2-bromo-1-ethanol + bromide
-
about 25% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
1,2-dibromoethane + H2O
2-bromo-1-ethanol + bromide
-
the highest specific activity of enzyme form eHLD-C is determined for 1,2-dibromoethane and 1-bromo-2-chloroethane
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
high activity
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
Agrobacterium tumefaciens C58 / ATCC 33970
high activity
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
1100% of the activity with 1-chlorobutane
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
1100% of the activity with 1-chlorobutane
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
42% of the activity with 4-chlorobutanol
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
about 45% of the activity compared to 4-bromobutyronitrile
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
high activity
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
about 90% of the specific activity compared to 1-bromobutane, enzyme form DmrA
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
strong dehalogenase activity
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
about 90% of the specific activity compared to 1-bromobutane, enzyme form DmrA
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
high activity
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
P51698
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
94% of the activity with 1,2-dichloroethane
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
78% of the activity with 1,2-dichloroethane
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
-
?
1,2-dibromoethane + H2O
2-bromoethanol + bromide
-
-
?
1,2-dibromopropane + H2O
2-bromo-1-propanol + bromide
about 15% of the activity compared to 4-bromobutyronitrile
-
?
1,2-dibromopropane + H2O
2-bromo-1-propanol + bromide
-
low activity
-
?
1,2-dibromopropane + H2O
2-bromo-1-propanol + bromide
-
low activity
-
?
1,2-dibromopropane + H2O
2-bromo-1-propanol + bromide
-
about 65% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1,2-dibromopropane + H2O
2-bromopropan-1-ol + bromide
-
-
?
1,2-dibromopropane + H2O
2-bromopropan-1-ol + bromide
-
-
?
1,2-dibromopropane + H2O
2-bromopropanol + bromide
high activity
-
?
1,2-dibromopropane + H2O
2-bromopropanol + bromide
low activity
-
?
1,2-dichloroethane + H2O
1,2-ethanediol + chloride
-
-
?
1,2-dichloroethane + H2O
2-chloro-1-ethanol + chloride
-
-
?
1,2-dichloroethane + H2O
2-chloro-1-ethanol + chloride
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
low activity
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
low activity
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
low activity
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
low activity
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
low activity
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
low activity
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
highest activity
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
analysis of dynamic and electrostatic effects
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
highest activity
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
-
?
1,2-dichloroethane + H2O
2-chloroethanol + chloride
-
-
?
1,2-dichloropropane + H2O
2-chloro-1-propanol + chloride
-
based on the SN2 nucleophilic substitution mechanism, the dechlorination process is identified as the rate-determining step in LinB-catalyzed degradation of 1,2-dichloropropane. LinB is enantioselective when metabolizing the racemic mixture 1,2-dichloropropane. The overall activation barrier for degradation of (S)-1,2-dichloropropane is 5.2 kcal/mol higher than that of (R)-1,2-dichloropropane
-
?
1,2-dichloropropane + H2O
2-chloro-1-propanol + chloride
-
based on the SN2 nucleophilic substitution mechanism, the dechlorination process is identified as the rate-determining step in LinB-catalyzed degradation of 1,2-dichloropropane. LinB is enantioselective when metabolizing the racemic mixture 1,2-dichloropropane. The overall activation barrier for degradation of (S)-1,2-dichloropropane is 5.2 kcal/mol higher than that of (R)-1,2-dichloropropane
-
?
1,2-dichloropropane + H2O
2-chloro-1-propanol + chloride
-
less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1,2-dichloropropane + H2O
2-chloropropan-1-ol + chloride
-
low activity
-
?
1,2-dichloropropane + H2O
2-chloropropan-1-ol + chloride
-
low activity
-
?
1,2-dichloropropane + H2O
2-chloropropan-1-ol + chloride
-
-
-
?
1,2-dichloropropane + H2O
2-chloropropan-1-ol + chloride
-
-
?
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O
2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
-
-
?
1,3,4,6-tetrachloro-1,4,cyclohexadiene + 2 H2O
2,5-dichloro-2,5-cyclohexadiene-1,4-diol + 2 chloride
-
-
-
?
1,3-dibromo-2-methylpropane + H2O
3-bromo-2-methylpropan-1-ol + bromide
-
desymmetrisation of a dihaloalkane is followed by kinetic resolution of the chiral haloalcohol that is produced in the first step, increase of the enantiomeric excess of the respective haloalcohol
-
?
1,3-dibromo-2-methylpropane + H2O
3-bromo-2-methylpropan-1-ol + bromide
-
desymmetrisation of a dihaloalkane is followed by kinetic resolution of the chiral haloalcohol that is produced in the first step, increase of the enantiomeric excess of the respective haloalcohol
-
?
1,3-dibromo-2-phenylpropane + H2O
?
-
desymmetrisation of a dihaloalkane is followed by kinetic resolution of the chiral haloalcohol that is produced in the first step, increase of the enantiomeric excess of the respective haloalcohol
-
?
1,3-dibromo-2-phenylpropane + H2O
?
-
desymmetrisation of a dihaloalkane is followed by kinetic resolution of the chiral haloalcohol that is produced in the first step, increase of the enantiomeric excess of the respective haloalcohol
-
?
1,3-dibromo-2-propanol + H2O
3-bromo-1,2-propandiol
-
-
-
?
1,3-dibromopropane + H2O
1,3-propanediol + bromide
-
-
?
1,3-dibromopropane + H2O
3-bromo-1-propanol + bromide
-
-
?
1,3-dibromopropane + H2O
3-bromo-1-propanol + bromide
Agrobacterium tumefaciens C58 / ATCC 33970
-
-
?
1,3-dibromopropane + H2O
3-bromo-1-propanol + bromide
-
high activity
-
?
1,3-dibromopropane + H2O
3-bromo-1-propanol + bromide
-
high activity
-
?
1,3-dibromopropane + H2O
3-bromo-1-propanol + bromide
-
best substrate, the release of product 3-bromo-1-propanol is the rate limiting step
-
?
1,3-dibromopropane + H2O
3-bromo-1-propanol + bromide
-
the highest specific activity of enzyme form eHLD-B is determined for 1,3-dibromopropane and 1-bromo-3-chloropropane
-
?
1,3-dibromopropane + H2O
3-bromo-propanol + bromide
-
-
?
1,3-dibromopropane + H2O
3-bromo-propanol + bromide
-
-
?
1,3-dibromopropane + H2O
3-bromopropane + bromide
-
preferred substrate
-
?
1,3-dibromopropane + H2O
3-bromopropanol + bromide
-
-
?
1,3-dibromopropane + H2O
3-bromopropanol + bromide
-
420% of the activity with 1-chlorobutane
-
?
1,3-dibromopropane + H2O
3-bromopropanol + bromide
high activity
-
?
1,3-dibromopropane + H2O
3-bromopropanol + bromide
high activity
-
?
1,3-dibromopropane + H2O
3-bromopropanol + bromide
-
700% of the activity with 1-chlorobutane
-
?
1,3-dibromopropane + H2O
3-bromopropanol + bromide
-
high activity
-
?
1,3-dibromopropane + H2O
3-bromopropanol + bromide
-
-
?
1,3-dichloropropane + H2O
3-chloropropanol + chloride
-
-
?
1,3-dichloropropane + H2O
?
-
21% of the activity with 4-chlorobutanol
-
?
1,3-dichloropropane + H2O
?
-
21% of the activity with 4-chlorobutanol
-
?
1,3-dichloropropane + H2O
?
-
80% of the activity with 1,2-dichloroethane
-
?
1,3-diiodopropane + H2O
3-iodo-1-propanol + iodide
about 25% of the activity compared to 4-bromobutyronitrile
-
?
1,3-diiodopropane + H2O
3-iodo-1-propanol + iodide
-
4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1,3-diiodopropane + H2O
3-iodo-1-propanol + iodide
-
about 35% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
1,5-dichloropentane + H2O
5-chloro-1-pentanol + chloride
-
low activity
-
?
1,5-dichloropentane + H2O
5-chloro-1-pentanol + chloride
-
3% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1,5-dichloropentane + H2O
5-chloro-1-pentanol + chloride
-
about 45% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
1,5-dichloropentane + H2O
5-chloropentanol + chloride
-
-
?
1,5-dichloropentane + H2O
5-chloropentanol + chloride
low activity
-
?
1,5-dichloropentane + H2O
5-chloropentanol + chloride
-
-
-
?
1,6-dibromohexane + H2O
6-bromoethanol + bromide
about 25% of the specific activity compared to 1-bromobutane, enzyme form DmrA
-
?
1,6-dibromohexane + H2O
6-bromoethanol + bromide
about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB
-
?
1,6-dibromohexane + H2O
6-bromohexanol + bromide
-
-
?
1,6-dibromohexane + H2O
6-bromohexanol + bromide
-
-
?
1-bromo-2-chloroethane + H2O
2-chloro-1-ethanol + bromide
-
about 10% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
1-bromo-2-chloroethane + H2O
2-chloro-1-ethanol + bromide
-
about 85% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1-bromo-2-chloroethane + H2O
2-chloroethanol + bromide
high activity
-
?
1-bromo-2-chloroethane + H2O
2-chloroethanol + bromide
-
-
-
?
1-bromo-2-chloroethane + H2O
2-chloroethanol + bromide
-
high activity
-
?
1-bromo-3-chloropropane + H2O
3-chloro-1-propanol + bromide
about 25% of the activity compared to 4-bromobutyronitrile
-
?
1-bromo-3-chloropropane + H2O
3-chloro-1-propanol + bromide
-
about 75% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1-bromo-3-chloropropane + H2O
3-chloro-1-propanol + bromide
-
about 80% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
1-bromo-3-chloropropane + H2O
3-chloropropanol + bromide
-
-
?
1-bromo-3-chloropropane + H2O
3-chloropropanol + bromide
high activity
-
?
1-bromo-3-chloropropane + H2O
3-chloropropanol + bromide
-
-
-
?
1-bromobutane + H2O
1-butanol + bromide
high activity
-
?
1-bromobutane + H2O
1-butanol + bromide
about 10% of the activity compared to 4-bromobutyronitrile
-
?
1-bromobutane + H2O
1-butanol + bromide
best substrate
-
?
1-bromobutane + H2O
1-butanol + bromide
strong dehalogenase activity
-
?
1-bromobutane + H2O
1-butanol + bromide
best substrate
-
?
1-bromobutane + H2O
1-butanol + bromide
preferred substrate
-
?
1-bromobutane + H2O
1-butanol + bromide
-
about 35% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1-bromobutane + H2O
1-butanol + bromide
-
about 60% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
1-bromobutane + H2O
butanol + bromide
-
25% of the activity with 4-chlorobutanol
-
?
1-bromobutane + H2O
butanol + bromide
-
reaction in aqueous phase, 98% of the activity with 1-chlorobutane, reaction in gaseous phase, 98% of the activity with 1-chlorobutane
-
?
1-bromobutane + H2O
butanol + bromide
-
reaction in aqueous phase, 98% of the activity with 1-chlorobutane, reaction in gaseous phase, 98% of the activity with 1-chlorobutane
-
?
1-bromobutane + H2O
n-butanol + bromide
-
180% of the activity with 1-chlorobutane
-
?
1-bromobutane + H2O
n-butanol + bromide
-
180% of the activity with 1-chlorobutane
-
?
1-bromobutane + H2O
n-butanol + bromide
-
630% of the activity with 1-chlorobutane
-
?
1-bromobutane + H2O
n-butanol + bromide
-
630% of the activity with 1-chlorobutane
-
?
1-bromohexane + H2O
1-hexanol + bromide
the enzyme shows highest activity toward 1-bromohexane
-
?
1-bromohexane + H2O
1-hexanol + bromide
best substrate
-
?
1-bromohexane + H2O
1-hexanol + bromide
about 35% of the specific activity compared to 1-bromobutane, enzyme form DmrA
-
?
1-bromohexane + H2O
1-hexanol + bromide
clearly preferred substrate
-
?
1-bromohexane + H2O
1-hexanol + bromide
enzyme form DmrB
-
?
1-bromohexane + H2O
1-hexanol + bromide
clearly preferred substrate
-
?
1-bromohexane + H2O
1-hexanol + bromide
best substrate
-
?
1-bromohexane + H2O
1-hexanol + bromide
-
4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1-bromohexane + H2O
1-hexanol + bromide
-
about 20% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
1-bromohexane + H2O
hexanol + bromide
-
reaction in aqueous phase, 87% of the activity with 1-chlorobutane, reaction in gaseous phase, 301% of the activity with 1-chlorobutane
-
?
1-bromopentane + H2O
1-pentanol + bromide
low activity
-
?
1-bromopentane + H2O
1-pentanol + bromide
about 15% of the specific activity compared to 1-bromohexane, enzyme form DmrB
-
?
1-bromopentane + H2O
1-pentanol + bromide
about 85% of the specific activity compared to 1-bromobutane, enzyme form DmrA
-
?
1-bromopentane + H2O
1-pentanol + bromide
strong dehalogenase activity
-
?
1-bromopentane + H2O
1-pentanol + bromide
low activity
-
?
1-bromopropane + H2O
1-propanol + bromide
about 60% of the specific activity compared to 1-bromobutane, enzyme form DmrA
-
?
1-bromopropane + H2O
propanol + bromide
-
29% of the activity with 1,2-dichloroethane
-
?
1-chloro-2,2-dimethylpropane + H2O
2,2-dimethylpropan-1-ol + chloride
-
-
-
?
1-chloro-2,2-dimethylpropane + H2O
2,2-dimethylpropan-1-ol + chloride
-
-
-
?
1-chloro-2-(2-chloroethoxy)ethane + H2O
?
-
-
-
?
1-chloro-2-methylpropane + H2O
2-methylpropanol + chloride
-
-
?
1-chloro-2-methylpropane + H2O
2-methylpropanol + chloride
-
20% of the activity with 4-chlorobutanol
-
?
1-chlorobutane + H2O
1-butanol + chloride
less than 5% of the activity compared to 4-bromobutyronitrile
-
?
1-chlorobutane + H2O
1-butanol + chloride
-
-
-
?
1-chlorobutane + H2O
1-butanol + chloride
-
less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1-chlorobutane + H2O
butanol + chloride
-
22% of the activity with 4-chlorobutanol
-
?
1-chlorobutane + H2O
butanol + chloride
-
22% of the activity with 4-chlorobutanol
-
?
1-chlorobutane + H2O
butanol + chloride
-
-
?
1-chlorobutane + H2O
butanol + chloride
-
-
?
1-chlorobutane + H2O
butanol + chloride
-
-
-
?
1-chlorobutane + H2O
butanol + chloride
-
31% of the activity with 1,2-dichloroethane
-
?
1-chlorodecane + H2O
n-decanol + chloride
-
52% of the activity with 1-chlorobutane
-
?
1-chlorodecane + H2O
n-decanol + chloride
-
110% of the activity with 1-chlorobutane
-
?
1-chloroheptane + H2O
heptanol + chloride
-
22% of the activity with 4-chlorobutanol
-
?
1-chlorohexane + H2O
1-hexanol + chloride
-
about 20% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B
-
?
1-chlorohexane + H2O
1-hexanol + chloride
-
less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C
-
?
1-chlorohexane + H2O
hexanol + chloride
-
15% of the activity with 4-chlorobutanol
-
?
1-chlorohexane + H2O
hexanol + chloride
-
reaction in aqueous phase, 100% of the activity with 1-chlorobutane, reaction in gaseous phase, 194% of the activity with 1-chlorobutane
-
?
1-chlorohexane + H2O
hexanol + chloride
-
reaction in aqueous phase, 100% of the activity with 1-chlorobutane, reaction in gaseous phase, 194% of the activity with 1-chlorobutane
-
?
1-chlorohexane + H2O
hexanol + chloride
-
best substrate
-
?
1-chlorohexane + H2O
n-hexanol + chloride
-
210% of the activity with 1-chlorobutane
-
?
1-chlorohexane + H2O
n-hexanol + chloride
-
210% of the activity with 1-chlorobutane
-
?
1-chlorohexane + H2O
n-hexanol + chloride
-
110% of the activity with 1-chlorobutane
-
?
1-chlorohexane + H2O
n-hexanol + chloride
-
110% of the activity with 1-chlorobutane
-
?
1-chlorohexane + H2O
n-hexanol + chloride
-
low activity
-
?
1-chlorooctane + H2O
octanol + chloride
-
18% of the activity with 4-chlorobutanol
-
?
1-chloropentane + H2O
n-pentanol + chloride
-
140% of the activity with 1-chlorobutane
-
?
1-chloropentane + H2O
n-pentanol + chloride
-
120% of the activity with 1-chlorobutane
-
?
1-chloropentane + H2O
pentanol + chloride
-
20% of the activity with 4-chlorobutanol
-
?
1-chloropentane + H2O
pentanol + chloride
-
reaction in aqueous phase, 101% of the activity with 1-chlorobutane, reaction in gaseous phase, 186% of the activity with 1-chlorobutane
-
?
1-chloropentane + H2O
pentanol + chloride
-
reaction in aqueous phase, 101% of the activity with 1-chlorobutane, reaction in gaseous phase, 186% of the activity with 1-chlorobutane
-
?
1-chloropropane + H2O
propanol + chloride
-
5% of the activity with 4-chlorobutanol
-
?
1-chloropropane + H2O
propanol + chloride
-
5% of the activity with 4-chlorobutanol
-
?
1-chloropropane + H2O
propanol + chloride
-
51% of the activity with 1,2-dichloroethane
-
?
1-chloropropane + H2O
propanol + chloride
-
51% of the activity with 1,2-dichloroethane
-
?
1-haloalkane + H2O
a primary alcohol + halide
subfamily HLD-II, substrates larger, beta-substituted
-
?
1-haloalkane + H2O
a primary alcohol + halide
subfamily HLD-II, substrates larger, beta-substituted
-
?
1-haloalkane + H2O
a primary alcohol + halide
subfamily HLD-II, substrates larger, beta-substituted
-
?
1-haloalkane + H2O
a primary alcohol + halide
-
subfamily HLD-I, substrates small, terminally halogenated
-
?
1-haloalkane + H2O
a primary alcohol + halide
-
subfamily HLD-I, substrates small, terminally halogenated
-
?
1-haloalkane + H2O
a primary alcohol + halide
-
subfamily HLD-II, substrates larger, beta-substituted
-
?
1-haloalkane + H2O
a primary alcohol + halide
-
subfamily HLD-III, unknown substrates
-
?
1-haloalkane + H2O
a primary alcohol + halide
-
-
?
1-haloalkane + H2O
a primary alcohol + halide
-
-
?
1-haloalkane + H2O
a primary alcohol + halide
-
-
?
1-haloalkane + H2O
a primary alcohol + halide
-
subfamily HLD-II, substrates larger, beta-substituted
-
?
1-haloalkane + H2O
a primary alcohol + halide
subfamily HLD-II, substrates larger, beta-substituted
-
?
1-haloalkane + H2O
a primary alcohol + halide
subfamily HLD-III, unknown substrates
-
?
1-haloalkane + H2O
a primary alcohol + halide
subfamily HLD-II, substrates larger, beta-substituted
-
?
1-haloalkane + H2O
a primary alcohol + halide
P51698
-
-
?
1-haloalkane + H2O
a primary alcohol + halide
subfamily HLD-I, substrates small, terminally halogenated
-
?
1-iodobutane + H2O
1-butanol + iodide
<