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Information on EC 3.8.1.5 - haloalkane dehalogenase
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3.8.1.5 haloalkane dehalogenaseIUBMB Comments
Acts on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds.
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Word Map
- 3.8.1.5
- xanthobacter
- autotrophicus
-
dehalogenation
- 1,2-dichloroethane
- halide
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carbon-halogen
- 1,2-dibromoethane
- sphingomonas
- paucimobilis
- 1,2,3-trichloropropane
- synthesis
- hexachlorocyclohexane
- rhodochrous
- sphingobium
- environmental protection
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alkyl-enzyme
-
haloacid
- 1-chlorobutane
- 2-chloroethanol
-
ncimb
-
chloroalkane
- dehydrochlorinase
- epichlorohydrine
- gamma-hexachlorocyclohexane
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halotag
- biotechnology
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alpha/beta-hydrolase
- haloalcohols
- agriculture
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halide-binding
- degradation
- industry
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
haloalkane dehalogenase, haloalkane dehalogenase linb, dhaa31, hld-i, rv2579, ehld-c, linbut, ylehd, 1-chlorohexane halidohydrolase, ehld-b, 
more
topREACTION 
REACTION DIAGRAM
COMMENTARY 
ORGANISM
UNIPROT
LITERATURE
1-haloalkane + H2O = a primary alcohol + halide
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1-haloalkane + H2O = a primary alcohol + halide
GJ10, three-step reaction mechanism with covalent alkyl-enzyme ester intermediate
1-haloalkane + H2O = a primary alcohol + halide
GJ10, four-step reaction mechanism with covalent ester intermediate
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1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
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1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
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1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
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1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
1-haloalkane + H2O = a primary alcohol + halide
GJ10, detailed reaction mechanism and energetics calculated from computational model
1-haloalkane + H2O = a primary alcohol + halide
two-step mechanism involving an ester intermediate covalently bound at Asp124, His 289 is important for hydrolysis
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1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Asn38 is involved, SN2 reaction mechanism, modeling, overview
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1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Asn41 is involved, SN2 reaction mechanism, modeling, overview
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1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Glu56 is involved, SN2 reaction mechanism, modeling, overview
1-haloalkane + H2O = a primary alcohol + halide
acts on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds
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1-haloalkane + H2O = a primary alcohol + halide
catalytic nucleophile Asp108, which is the most disordered catalytic residue, catalytic base His272, structure-based reaction mechanism, active site structure, overview
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1-haloalkane + H2O = a primary alcohol + halide
low activity with small, chlorinated alkanes, importance of active site water molecules and reaction products in molecular docking
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1-haloalkane + H2O = a primary alcohol + halide
substrate specificity is influenced by the size and shape of their entrance tunnel, surface residue Leu177 is involved and located at the tunnel opening
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1-haloalkane + H2O = a primary alcohol + halide
activation energy of reaction 59.5 kJ/mol
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1-haloalkane + H2O = a primary alcohol + halide
catalytic triad consists of D123, H279, D250, with a first halide-stabilizing W124 and a secong stabilizing residue W164. Two-step reaction mechanism
1-haloalkane + H2O = a primary alcohol + halide
H272 singly protonated at Ndelta1 and D108 in conformation A give the most exothermic reactionwith DeltaH of -22 kcal/mol
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1-haloalkane + H2O = a primary alcohol + halide
hydrogen bond interactions between substrate and the environment are significantly different in aqueous solution and in the enzyme. Structure of the enzyme active site provides a more adequate interaction pattern for the reaction process
1-haloalkane + H2O = a primary alcohol + halide
Asp103, Glu127 and His280 are involved in the catalytic reaction, and two H-bond donating residues, Asn38 and Trp104, are involved in stabilization of a halogen group of the substrate
1-haloalkane + H2O = a primary alcohol + halide
reaction mechanism, overview. The bromide release is the rate-limiting step of the catalytic cycle, overview
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1-haloalkane + H2O = a primary alcohol + halide
structural basis for its reaction mechanism
1-haloalkane + H2O = a primary alcohol + halide
Asp103, Glu127 and His280 are involved in the catalytic reaction, and two H-bond donating residues, Asn38 and Trp104, are involved in stabilization of a halogen group of the substrate
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1-haloalkane + H2O = a primary alcohol + halide
catalytic nucleophile Asp108, which is the most disordered catalytic residue, catalytic base His272, structure-based reaction mechanism, active site structure, overview
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-
1-haloalkane + H2O = a primary alcohol + halide
H272 singly protonated at Ndelta1 and D108 in conformation A give the most exothermic reactionwith DeltaH of -22 kcal/mol
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-
1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
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-
1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Asn38 is involved, SN2 reaction mechanism, modeling, overview
-
-
1-haloalkane + H2O = a primary alcohol + halide
low activity with small, chlorinated alkanes, importance of active site water molecules and reaction products in molecular docking
-
-
1-haloalkane + H2O = a primary alcohol + halide
substrate specificity is influenced by the size and shape of their entrance tunnel, surface residue Leu177 is involved and located at the tunnel opening
-
-
1-haloalkane + H2O = a primary alcohol + halide
catalytic triad consists of D123, H279, D250, with a first halide-stabilizing W124 and a secong stabilizing residue W164. Two-step reaction mechanism
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1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
-
1-haloalkane + H2O = a primary alcohol + halide
structural basis for its reaction mechanism
Agrobacterium tumefaciens C58 / ATCC 33970
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1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
-
1-haloalkane + H2O = a primary alcohol + halide
two-step mechanism involving an ester intermediate covalently bound at Asp124, His 289 is important for hydrolysis
-
-
1-haloalkane + H2O = a primary alcohol + halide
GJ10, four-step reaction mechanism with covalent ester intermediate
-
-
1-haloalkane + H2O = a primary alcohol + halide
alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction
-
-
1-haloalkane + H2O = a primary alcohol + halide
GJ10, three-step reaction mechanism with covalent alkyl-enzyme ester intermediate
-
-
1-haloalkane + H2O = a primary alcohol + halide
GJ10, detailed reaction mechanism and energetics calculated from computational model
-
-
1-haloalkane + H2O = a primary alcohol + halide
3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Glu56 is involved, SN2 reaction mechanism, modeling, overview
-
-
1-haloalkane + H2O = a primary alcohol + halide
reaction mechanism, overview. The bromide release is the rate-limiting step of the catalytic cycle, overview
-
-
1-haloalkane + H2O = a primary alcohol + halide
distinction of 3 different substrate classes for haloalkane dehalogenases after profound statistical reaction rate analysis
-
-
1-haloalkane + H2O = a primary alcohol + halide
activation energy of reaction 59.5 kJ/mol
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