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Literature summary for 3.8.1.5 extracted from

  • Drienovska, I.; Chovancova, E.; Koudelakova, T.; Damborsky, J.; Chaloupkova, R.
    Biochemical characterization of a novel haloalkane dehalogenase from a cold-adapted bacterium (2012), Appl. Environ. Microbiol., 78, 4995-4998.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic analysis with substrates 1-bromobutane and 1,3-dibromopropane, positive cooperative substrate binding, overview Psychrobacter cryohalolentis
3.83
-
 1-bromobutane pH 8.7, 37°C Psychrobacter cryohalolentis
5.77
-
 1,3-dibromopropane pH 8.7, 37°C Psychrobacter cryohalolentis

Organism

Organism UniProt Comment Textmining
Psychrobacter cryohalolentis
-
-
-
Psychrobacter cryohalolentis K5
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1-bromomethyl)cyclohexane + H2O low activity Psychrobacter cryohalolentis ?
-
 ?
1,2-dibromopropane + H2O low activity Psychrobacter cryohalolentis 2-bromo-1-propanol + bromide
-
 ?
1,2-dibromopropane + H2O low activity Psychrobacter cryohalolentis K5 2-bromo-1-propanol + bromide
-
 ?
1,3-dibromopropane + H2O high activity Psychrobacter cryohalolentis 3-bromo-1-propanol + bromide
-
 ?
1,3-dibromopropane + H2O high activity Psychrobacter cryohalolentis K5 3-bromo-1-propanol + bromide
-
 ?
1,3-diiodopropane + H2O
-
 Psychrobacter cryohalolentis 3-iodo-1-propanol + iodide
-
 ?
1,5-dichloropentane + H2O low activity Psychrobacter cryohalolentis 5-chloro-1-pentanol + chloride
-
 ?
1-bromo-2-chloroethane + H2O low activity Psychrobacter cryohalolentis 2-chloroethanol + bromide
-
 ?
1-bromo-3-chloropropane + H2O
-
 Psychrobacter cryohalolentis ?
-
 ?
1-bromobutane + H2O high activity Psychrobacter cryohalolentis 1-butanol + bromide
-
 ?
1-bromohexane + H2O high activity Psychrobacter cryohalolentis 1-hexanol + bromide
-
 ?
1-chlorohexane + H2O low activity Psychrobacter cryohalolentis n-hexanol + chloride
-
 ?
1-chlorohexane + H2O low activity Psychrobacter cryohalolentis K5 n-hexanol + chloride
-
 ?
1-iodobutane + H2O
-
 Psychrobacter cryohalolentis n-butanol + iodide
-
 ?
1-iodobutane + H2O
-
 Psychrobacter cryohalolentis K5 n-butanol + iodide
-
 ?
1-iodohexane + H2O
-
 Psychrobacter cryohalolentis n-hexanol + iodide
-
 ?
1-iodopropane + H2O
-
 Psychrobacter cryohalolentis n-propanol + iodide
-
 ?
2-iodobutane + H2O
-
 Psychrobacter cryohalolentis 2-butanol + iodide
-
 ?
3-chloro-2-methylpropene + H2O low activity Psychrobacter cryohalolentis ?
-
 ?
4-bromobutyronitrile + H2O
-
 Psychrobacter cryohalolentis ?
-
 ?
additional information substrate specificity profiles of DpcA, DpcA prefers brominated or iodinated substrates over chlorinated ones, overview Psychrobacter cryohalolentis ?
-
 ?
additional information substrate specificity profiles of DpcA, DpcA prefers brominated or iodinated substrates over chlorinated ones, overview Psychrobacter cryohalolentis K5 ?
-
 ?

Subunits

Subunits Comment Organism
More sequence-based screening and enzyme identification, sequence comparisons, folding and secondary structure of DpcA, overview Psychrobacter cryohalolentis

Synonyms

Synonyms Comment Organism
DpcA
-
 Psychrobacter cryohalolentis
HLD
-
 Psychrobacter cryohalolentis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50 with substrate 1-bromobutane Psychrobacter cryohalolentis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 1-bromobutane pH 8.7, 37°C Psychrobacter cryohalolentis
2.72
-
 1,3-dibromopropane pH 8.7, 37°C Psychrobacter cryohalolentis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
 with substrate 1-bromobutane Psychrobacter cryohalolentis

pH Range

pH Minimum pH Maximum Comment Organism
6.4 9.6 DpcA loses all activity below pH 6.4 and above pH 9.6 Psychrobacter cryohalolentis

General Information

General Information Comment Organism
evolution DpcA is a member of the haloalkane dehalogenase family Psychrobacter cryohalolentis