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Information on EC 3.6.1.10 - endopolyphosphatase
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3.6.1.10 endopolyphosphataseIUBMB Comments
The product contains 4 or 5 phosphate residues.
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The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Synonyms
endopolyPase, metaphosphatase, phosphatase, endopoly-, polymetaphosphatase, polyphosphatase, polyphosphate depolymerase, PPN, Ppn1, short-chain polyphosphate, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
metaphosphatase
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-
-
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phosphatase, endopoly-
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-
-
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polymetaphosphatase
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-
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polyphosphatase
-
-
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polyphosphate depolymerase
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-
-
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PPN
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-
-
-
Ppn1
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
polyphosphate + n H2O = (n+1) oligophosphate
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-
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphorous acid anhydride hydrolysis
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-
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SYSTEMATIC NAME
IUBMB Comments
polyphosphate polyphosphohydrolase
The product contains 4 or 5 phosphate residues.
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CAS REGISTRY NUMBER
COMMENTARY
9024-86-6
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
polyphosphate 208 + H2O
oligophosphate
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-
the average polyphosphate chain lengths decrease from about 208 to about 10 phosphate residues
-
?
polyphosphate 208 + H2O
polyphosphate 193 + polyphosphate 15
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-
-
-
?
polyphosphate700 + H2O
phosphate + triphosphate
progression via intermediate chain length polyphosphates
product analysis
-
?
polyphosphate + H2O
oligophosphate
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polymetaphosphate
break down stops when the scission products are tetraphosphate or pentaphosphate
?
polyphosphate + H2O
oligophosphate
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distributive action on polyP750 produces shorter chains to a limit of about polyP60 as well as the more abundant release of polyP3, limit digestion product is polyP3
?
additional information
?
-
-
inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1
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additional information
?
-
null mutants in Ppn1 accumulate long-chain polyphosphates and are defective in growth in minimal media
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-
additional information
?
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the pathway of particular polyphosphates in yeast is different with endo- and exopolyphosphatase playing critical roles
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additional information
?
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enzyme is a bifunctional exo- and endopolyphosphatase, activities of ECs 3.6.1.11 and 3.6.1.10, respectively. No activity with diphosphate, ATP and 4-nitrophenyl phosphate
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
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-
inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1
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additional information
?
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Q04119
null mutants in Ppn1 accumulate long-chain polyphosphates and are defective in growth in minimal media
-
-
-
additional information
?
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-
the pathway of particular polyphosphates in yeast is different with endo- and exopolyphosphatase playing critical roles
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
Zn2+
additional information
Mg2+
in the presence of Mg2+, the enzyme shows a polyphosphate chain fragmentation activity
additional information
-
addition of 3 mM Mg2+ and 3 mM Co2+ has no effect on the cytosol endopolyphosphatase activity
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ATP
ATP inhibits while ADP activates endopolyphosphatase activity in the presence of Mg2+
additional information
-
the enzyme is insensitive to 5 mM fluoride
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ADP
ATP inhibits while ADP activates endopolyphosphatase activity in the presence of Mg2+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional exo- and endopolyphosphatase, EC 3.6.1.11 and EC 3.6.1.10
SwissProt
brenda
strain CRX, CRY, CRN, and CNX derived from strain W303-1A
SwissProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
activity is greater in mycorrhizal roots of Allium cepa than in non-mycorrhizal roots
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Show AA Sequence and Transmembrane Helices (548 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
34500
4 * 34500, native enzyme, SDS-PAGE and mass spectrometry
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
tetramer
4 * 34500, native enzyme, SDS-PAGE and mass spectrometry
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
proteolytic modification
proteolytic modification
both the C-terminus and the N-terminus of Ppn1 are cleaved during enzyme activation
proteolytic modification
PPN1 gene encodes a polypeptide with the predicted molecular mass of 78 kDa, the monomer molecular mass of the mature PPN1 is about 33-35 kDa
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1
additional information
null mutants in Ppn1 accumulate long-chain polyphosphates and are defective in growth in minimal media
additional information
construction of Ppn1 mutants, overview
additional information
-
inactivation of gene ppn1 encoding the endopolyphosphatase PPN1 togehther with inactivation of exopolyphosphatase PPX1 leads to a two-fold increase in high-molecular weight alkali-soluble polyphosphates in yeast cells
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
DEAE Toyopearl 650M column chromatography, heparin agarose column chromatography, and Mono Q column chromatography
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native enzyme from exopolyphosphatase-deficient mutant strain CRX by heparin affinity and phosphocellulose chromatography, 3820fold to homogeneity
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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
gene ppn1, DNA and amino acid sequence determination and analysis, expression of wild-type pre-Ppn1 and mutants in Escherichia coli and in HEK-293 cells
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
the overexpression of the processed form of the enzyme should provide a unique and powerful reagent to analyze inorganic polyphosphate when the chain termini are unavailable to the actions of polyPase and polyP kinase
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Malmgren, H.
Enzymatic breakdown of polymetaphosphate V. Purification and specificity of the enzyme
Acta Chem. Scand.
6
16-26
1952
Aspergillus niger
-
brenda
Capaccio, L.C.M.; Callow, J.A.
The enzymes of polyphosphate metabolism in vesicular-arbuscular mycorrhizas
New Phytol.
91
81-91
1982
Funneliformis mosseae
-
brenda
Kritsky, M.S.; Chernyshova, E.K.
The study of polyphosphate depolymerase activity of Neurospora crassa
React. Mech. Control Prop. Phosphotransferases, Int. Symp. Meeting, Akad. Verl. Berlin
49-56
1971
Neurospora crassa, Neurospora crassa 28610
-
brenda
Kowalczyk, T.H.; Phillips, N.F.B.
Determination of endopolyphosphatase using polyphosphate glucokinase
Anal. Biochem.
212
194-205
1993
Saccharomyces cerevisiae
brenda
Kumble, K.; Kornberg, A.
Endopolyphosphatase for long chain inorganic polyphosphate in yeast and mammals
J. Biol. Chem.
271
27146-27151
1996
Caenorhabditis elegans, Chlamydomonas sp., Dictyostelium discoideum, Drosophila melanogaster, Giardia intestinalis, Neurospora crassa, Phaeodactylum tricornutum, Pyrococcus islandicum, Rattus norvegicus, Saccharomyces cerevisiae, Sulfolobus acidocaldarius, Synechococcus sp.
brenda
Lichko, L.; Kulakovskaya, T.; Kulaev, I.
Inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1 in Saccharomyces cerevisiae
Biochim. Biophys. Acta
1674
98-102
2004
Saccharomyces cerevisiae
brenda
Sethuraman, A.; Rao, N.N.; Kornberg, A.
The endopolyphosphatase gene: essential in Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
98
8542-8547
2001
Saccharomyces cerevisiae (Q04119), Saccharomyces cerevisiae
brenda
Shi, X.; Kornberg, A.
Endopolyphosphatase in Saccharomyces cerevisiae undergoes post-translational activations to produce short-chain polyphosphates
FEBS Lett.
579
2014-2018
2005
Saccharomyces cerevisiae (Q04119), Saccharomyces cerevisiae
brenda
Kulakovskaya, T.V.; Trilisenko, L.V.; Lichko, L.P.; Vagabov, V.M.; Kulaev, I.S.
The effect of inactivation of the exo- and endopolyphosphatase genes PPX1 and PPN1 on the level of different polyphosphates in the yeast Saccharomyces cerevisiae
Microbiology
75
25-28
2006
Saccharomyces cerevisiae
-
brenda
Lichko, L.P.; Kulakovskaya, T.V.; Kulakovskaya, E.V.; Kulaev, I.S.
Finding of endopolyphosphatase activity in the yeast Saccharomyces cerevisiae
Biochemistry
74
842-845
2009
Saccharomyces cerevisiae
brenda
Lichko, L.P.; Kulakovskaya, T.V.; Kulaev, I.S.
Properties of partially purified endopolyphosphatase of the yeast Saccharomyces cerevisiae
Biochemistry (Moscow)
75
1404-1407
2010
Saccharomyces cerevisiae
brenda
Andreeva, N.; Trilisenko, L.; Eldarov, M.; Kulakovskaya, T.
Polyphosphatase PPN1 of Saccharomyces cerevisiae: switching of exopolyphosphatase and endopolyphosphatase activities
PLoS ONE
10
e0119594
2015
Saccharomyces cerevisiae (Q04119), Saccharomyces cerevisiae
brenda
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