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Information on EC 3.6.1.10 - endopolyphosphatase for references in articles please use BRENDA:EC3.6.1.10Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
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polyphosphate + n H2O = (n+1) oligophosphate
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phosphorous acid anhydride hydrolysis
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polyphosphate polyphosphohydrolase
The product contains 4 or 5 phosphate residues.
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phosphatase, endopoly-
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polymetaphosphatase
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polyphosphate depolymerase
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short-chain polyphosphate
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Ppn1
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Chlamydomonas sp.
low activity
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low activity
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low activity
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Pyrococcus islandicum
low activity
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low activity
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low activity
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28610
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SwissProt
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bifunctional exo- and endopolyphosphatase, EC 3.6.1.11 and EC 3.6.1.10
SwissProt
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gene ppn1
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strain CRX, CRY, CRN, and CNX derived from strain W303-1A
SwissProt
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polyphosphate + H2O
oligophosphate
polyphosphate 208 + H2O
oligophosphate
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the average polyphosphate chain lengths decrease from about 208 to about 10 phosphate residues
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polyphosphate 208 + H2O
polyphosphate 193 + polyphosphate 15
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polyphosphate700 + H2O
phosphate + triphosphate
progression via intermediate chain length polyphosphates
product analysis
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additional information
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polyphosphate + H2O
oligophosphate
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polymetaphosphate
break down stops when the scission products are tetraphosphate or pentaphosphate
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
Chlamydomonas sp.
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
Pyrococcus islandicum
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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distributive action on polyP750 produces shorter chains to a limit of about polyP60 as well as the more abundant release of polyP3, limit digestion product is polyP3
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polyphosphate + H2O
oligophosphate
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polyphosphate + H2O
oligophosphate
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additional information
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inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1
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additional information
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null mutants in Ppn1 accumulate long-chain polyphosphates and are defective in growth in minimal media
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additional information
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the pathway of particular polyphosphates in yeast is different with endo- and exopolyphosphatase playing critical roles
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additional information
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enzyme is a bifunctional exo- and endopolyphosphatase, activities of ECs 3.6.1.11 and 3.6.1.10, respectively. No activity with diphosphate, ATP and 4-nitrophenyl phosphate
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additional information
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additional information
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inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1
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additional information
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Q04119
null mutants in Ppn1 accumulate long-chain polyphosphates and are defective in growth in minimal media
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additional information
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the pathway of particular polyphosphates in yeast is different with endo- and exopolyphosphatase playing critical roles
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Ba2+
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0.01-1 mM, activation
Mg2+
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activation
Mg2+
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0.01-1 mM, activation
Mg2+
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the enzyme is most active at 1.5 mM Mg2+
Mg2+
in the presence of Mg2+, the enzyme shows a polyphosphate chain fragmentation activity
Mn2+
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effective activation
Mn2+
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0.01-1 mM, activation
Mn2+
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optimal activation at 2.5 mM
Zn2+
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effective activation
Zn2+
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0.01-1 mM, activation
additional information
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addition of 3 mM Mg2+ and 3 mM Co2+ has no effect on the cytosol endopolyphosphatase activity
additional information
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not stimulated by Ca2+
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ATP
ATP inhibits while ADP activates endopolyphosphatase activity in the presence of Mg2+
CaCl2
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1 mM, 35% inhibition
diphosphate
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10 mM, complete inhibition
EDTA
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10 mM, complete inhibition
phosphate
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20 mM, 50% inhibition
ZnCl2
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1 mM, 35% inhibition
additional information
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the enzyme is insensitive to 5 mM fluoride
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ADP
ATP inhibits while ADP activates endopolyphosphatase activity in the presence of Mg2+
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65000
purified native Ppn1
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5.7
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hydrolysis of polymetaphosphate
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low activity
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low activity
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low activity
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low activity
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activity is greater in mycorrhizal roots of Allium cepa than in non-mycorrhizal roots
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at the end of the exponential phase of growth
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at the end of the exponential phase of growth
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34500
4 * 34500, native enzyme, SDS-PAGE and mass spectrometry
35000
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2 * 35000, SDS-PAGE
142000
native enzyme, gel filtration
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dimer
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2 * 35000, SDS-PAGE
tetramer
4 * 34500, native enzyme, SDS-PAGE and mass spectrometry
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proteolytic modification
both the C-terminus and the N-terminus of Ppn1 are cleaved during enzyme activation
proteolytic modification
PPN1 gene encodes a polypeptide with the predicted molecular mass of 78 kDa, the monomer molecular mass of the mature PPN1 is about 33-35 kDa
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35 - 40
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loss of activity above
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DEAE Toyopearl 650M column chromatography, heparin agarose column chromatography, and Mono Q column chromatography
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native enzyme from exopolyphosphatase-deficient mutant strain CRX by heparin affinity and phosphocellulose chromatography, 3820fold to homogeneity
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expression in Saccharomyces cerevisiae
gene ppn1, DNA and amino acid sequence determination and analysis, expression of wild-type pre-Ppn1 and mutants in Escherichia coli and in HEK-293 cells
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additional information
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inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1
additional information
null mutants in Ppn1 accumulate long-chain polyphosphates and are defective in growth in minimal media
additional information
construction of Ppn1 mutants, overview
additional information
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inactivation of gene ppn1 encoding the endopolyphosphatase PPN1 togehther with inactivation of exopolyphosphatase PPX1 leads to a two-fold increase in high-molecular weight alkali-soluble polyphosphates in yeast cells
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analysis
the overexpression of the processed form of the enzyme should provide a unique and powerful reagent to analyze inorganic polyphosphate when the chain termini are unavailable to the actions of polyPase and polyP kinase
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Malmgren, H.
Enzymatic breakdown of polymetaphosphate
Acta Chem. Scand.
6
16-26
1952
Aspergillus niger
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Capaccio, L.C.M.; Callow, J.A.
The enzymes of polyphosphate metabolism in vesicular-arbuscular mycorrhizas
New Phytol.
91
81-91
1982
Funneliformis mosseae
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Kritsky, M.S.; Chernyshova, E.K.
The study of polyphosphate depolymerase activity of Neurospora crassa
React. Mech. Control Prop. Phosphotransferases, Int. Symp. Meeting, Akad. Verl. Berlin
49-56
1971
Neurospora crassa, Neurospora crassa 28610
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Kowalczyk, T.H.; Phillips, N.F.B.
Determination of endopolyphosphatase using polyphosphate glucokinase
Anal. Biochem.
212
194-205
1993
Saccharomyces cerevisiae
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Kumble, K.; Kornberg, A.
Endopolyphosphatase for long chain inorganic polyphosphate in yeast and mammals
J. Biol. Chem.
271
27146-27151
1996
Caenorhabditis elegans, Chlamydomonas sp., Dictyostelium discoideum, Drosophila melanogaster, Giardia intestinalis, Neurospora crassa, Phaeodactylum tricornutum, Pyrococcus islandicum, Rattus norvegicus, Saccharomyces cerevisiae, Sulfolobus acidocaldarius, Synechococcus sp.
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Lichko, L.; Kulakovskaya, T.; Kulaev, I.
Inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1 in Saccharomyces cerevisiae
Biochim. Biophys. Acta
1674
98-102
2004
Saccharomyces cerevisiae
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Sethuraman, A.; Rao, N.N.; Kornberg, A.
The endopolyphosphatase gene: essential in Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
98
8542-8547
2001
Saccharomyces cerevisiae (Q04119), Saccharomyces cerevisiae
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Shi, X.; Kornberg, A.
Endopolyphosphatase in Saccharomyces cerevisiae undergoes post-translational activations to produce short-chain polyphosphates
FEBS Lett.
579
2014-2018
2005
Saccharomyces cerevisiae (Q04119), Saccharomyces cerevisiae
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Kulakovskaya, T.V.; Trilisenko, L.V.; Lichko, L.P.; Vagabov, V.M.; Kulaev, I.S.
The effect of inactivation of the exo- and endopolyphosphatase genes PPX1 and PPN1 on the level of different polyphosphates in the yeast Saccharomyces cerevisiae
Microbiology
75
25-28
2006
Saccharomyces cerevisiae
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Lichko, L.P.; Kulakovskaya, T.V.; Kulakovskaya, E.V.; Kulaev, I.S.
Finding of endopolyphosphatase activity in the yeast Saccharomyces cerevisiae
Biochemistry
74
842-845
2009
Saccharomyces cerevisiae
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Lichko, L.P.; Kulakovskaya, T.V.; Kulaev, I.S.
Properties of partially purified endopolyphosphatase of the yeast Saccharomyces cerevisiae
Biochemistry (Moscow)
75
1404-1407
2010
Saccharomyces cerevisiae
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Andreeva, N.; Trilisenko, L.; Eldarov, M.; Kulakovskaya, T.
Polyphosphatase PPN1 of Saccharomyces cerevisiae: switching of exopolyphosphatase and endopolyphosphatase activities
PLoS ONE
10
e0119594
2015
Saccharomyces cerevisiae (Q04119), Saccharomyces cerevisiae
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