Cloned (Comment) | Organism |
---|---|
gene ppn1, DNA and amino acid sequence determination and analysis, expression of wild-type pre-Ppn1 and mutants in Escherichia coli and in HEK-293 cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of Ppn1 mutants, overview | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34500 | - |
4 * 34500, native enzyme, SDS-PAGE and mass spectrometry | Saccharomyces cerevisiae |
142000 | - |
native enzyme, gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q04119 | strain CRX, CRY, CRN, and CNX derived from strain W303-1A | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | both the C-terminus and the N-terminus of Ppn1 are cleaved during enzyme activation | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
native enzyme from exopolyphosphatase-deficient mutant strain CRX by heparin affinity and phosphocellulose chromatography, 3820fold to homogeneity | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65000 | - |
purified native Ppn1 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polyphosphate700 + H2O | progression via intermediate chain length polyphosphates | Saccharomyces cerevisiae | phosphate + triphosphate | product analysis | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 34500, native enzyme, SDS-PAGE and mass spectrometry | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Ppn1 | - |
Saccharomyces cerevisiae |
short-chain polyphosphate | - |
Saccharomyces cerevisiae |