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Literature summary for 3.6.1.10 extracted from

  • Shi, X.; Kornberg, A.
    Endopolyphosphatase in Saccharomyces cerevisiae undergoes post-translational activations to produce short-chain polyphosphates (2005), FEBS Lett., 579, 2014-2018.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ppn1, DNA and amino acid sequence determination and analysis, expression of wild-type pre-Ppn1 and mutants in Escherichia coli and in HEK-293 cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of Ppn1 mutants, overview Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34500
-
4 * 34500, native enzyme, SDS-PAGE and mass spectrometry Saccharomyces cerevisiae
142000
-
native enzyme, gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q04119 strain CRX, CRY, CRN, and CNX derived from strain W303-1A
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification both the C-terminus and the N-terminus of Ppn1 are cleaved during enzyme activation Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
native enzyme from exopolyphosphatase-deficient mutant strain CRX by heparin affinity and phosphocellulose chromatography, 3820fold to homogeneity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65000
-
purified native Ppn1 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polyphosphate700 + H2O progression via intermediate chain length polyphosphates Saccharomyces cerevisiae phosphate + triphosphate product analysis ?

Subunits

Subunits Comment Organism
tetramer 4 * 34500, native enzyme, SDS-PAGE and mass spectrometry Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Ppn1
-
Saccharomyces cerevisiae
short-chain polyphosphate
-
Saccharomyces cerevisiae