Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.10 extracted from

  • Kumble, K.; Kornberg, A.
    Endopolyphosphatase for long chain inorganic polyphosphate in yeast and mammals (1996), J. Biol. Chem., 271, 27146-27151.
    View publication on PubMed
Search for more literature for 3.6.1.10

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 1 mM, 35% inhibition Saccharomyces cerevisiae
diphosphate 10 mM, complete inhibition Saccharomyces cerevisiae
EDTA 10 mM, complete inhibition Saccharomyces cerevisiae
phosphate 20 mM, 50% inhibition Saccharomyces cerevisiae
ZnCl2 1 mM, 35% inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000185
-
 polyP750
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
 Saccharomyces cerevisiae 5773
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Neurospora crassa
Mg2+ activates Saccharomyces cerevisiae
Mn2+
-
 Neurospora crassa
Mn2+ optimal activation at 2.5 mM Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Saccharomyces cerevisiae
80000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-
Chlamydomonas sp.
-
 low activity
-
Dictyostelium discoideum
-
-
-
Drosophila melanogaster
-
-
-
Giardia intestinalis
-
 low activity
-
Neurospora crassa
-
-
-
Phaeodactylum tricornutum
-
 low activity
-
Pyrococcus islandicum
-
 low activity
-
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-
Sulfolobus acidocaldarius
-
 low activity
-
Synechococcus sp.
-
 low activity
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
heart low activity Rattus norvegicus
-
kidney low activity Rattus norvegicus
-
liver low activity Rattus norvegicus
-
lung low activity Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polyP750 + H2O
-
 Saccharomyces cerevisiae polyP749 + phosphate
-
 ?
polyphosphate + H2O
-
 Drosophila melanogaster oligophosphate
-
 ?
polyphosphate + H2O
-
 Rattus norvegicus oligophosphate
-
 ?
polyphosphate + H2O
-
 Neurospora crassa oligophosphate
-
 ?
polyphosphate + H2O
-
 Giardia intestinalis oligophosphate
-
 ?
polyphosphate + H2O
-
 Dictyostelium discoideum oligophosphate
-
 ?
polyphosphate + H2O
-
 Sulfolobus acidocaldarius oligophosphate
-
 ?
polyphosphate + H2O
-
 Caenorhabditis elegans oligophosphate
-
 ?
polyphosphate + H2O
-
 Chlamydomonas sp. oligophosphate
-
 ?
polyphosphate + H2O
-
 Synechococcus sp. oligophosphate
-
 ?
polyphosphate + H2O
-
 Phaeodactylum tricornutum oligophosphate
-
 ?
polyphosphate + H2O
-
 Pyrococcus islandicum oligophosphate
-
 ?
polyphosphate + H2O
-
 Saccharomyces cerevisiae oligophosphate distributive action on polyP750 produces shorter chains to a limit of about polyP60 as well as the more abundant release of polyP3, limit digestion product is polyP3 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
20
-
 phosphate
-
 Saccharomyces cerevisiae