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The taxonomic range for the selected organisms is: Pseudomonas sp.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
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cyanuric acid + H2O
1-carboxybiuret
-
-
-
?
cyanuric acid + H2O
carboxybiuret
-
product spontaneously decarboxylates to give biuret and CO2. Therefore cyanuric acid hydrolase and barbiturase catalyze completely analogous reactions
-
?
cyanuric acid + H2O
biuret + CO2
additional information
?
-
cyanuric acid + H2O
biuret + CO2
-
high substrate specificity
-
-
?
cyanuric acid + H2O
biuret + CO2
-
2,4,6-trihydroxy-1,3,5-triazine
-
-
?
cyanuric acid + H2O
biuret + CO2
-
1,3,5-triazine-2,4,6(1H,3H,5H)-trione
-
-
?
cyanuric acid + H2O
biuret + CO2
-
2,4,6-trihydroxy-s-triazine
-
-
?
cyanuric acid + H2O
biuret + CO2
-
heterocyclic ring cleavage
-
-
?
cyanuric acid + H2O
biuret + CO2
-
fourth step in the six-step pathway for metabolism of melamine
-
-
?
cyanuric acid + H2O
biuret + CO2
-
first enzyme of degradative pathway of cyanuric acid
-
-
?
cyanuric acid + H2O
biuret + CO2
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
cyanuric acid + H2O
biuret + CO2
-
herterocyclic ring cleavage
-
-
?
cyanuric acid + H2O
biuret + CO2
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
hypothetical reaction mechanism consistent with a Ser85 nucleophile. Lys42 is a general base, activating Ser85 and promoting formation of a tetrahedral intermediate between Ser85 and the closest substrate carbonyl carbon, this then resolves into the acyl:enzyme intermediate following ring opening. Thereafter, a solvent water molecule is required to hydrolyse the acyl intermediate and regenerate the serine, liberating carboxybiuret
-
-
?
additional information
?
-
-
strain A, no activity with ammeline, 2,4-diamino-6-hydroxy-s-triazine, ammelide, 2-amino-4,6-dihydroxy-s-triazine, uracil, 2,4-dihydroxypyrimidine, 5,6-dihydrouracil, cytosine, 4-amino-2-hydroxypyrimidine, 2,4,5-trihydroxypyrimidine and barbituric acid, 2,4,6-trihydroxypyrimidine
-
-
?
additional information
?
-
-
strain A, high substrate specificity, no activity with any other s-triazine or hydroxypyrimidine compound
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
one step in the six-step pathway for metabolism of melamine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cyanuric acid + H2O
biuret + CO2
additional information
?
-
cyanuric acid + H2O
biuret + CO2
-
2,4,6-trihydroxy-1,3,5-triazine
-
-
?
cyanuric acid + H2O
biuret + CO2
-
1,3,5-triazine-2,4,6(1H,3H,5H)-trione
-
-
?
cyanuric acid + H2O
biuret + CO2
-
2,4,6-trihydroxy-s-triazine
-
-
?
cyanuric acid + H2O
biuret + CO2
-
fourth step in the six-step pathway for metabolism of melamine
-
-
?
cyanuric acid + H2O
biuret + CO2
-
first enzyme of degradative pathway of cyanuric acid
-
-
?
cyanuric acid + H2O
biuret + CO2
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
cyanuric acid + H2O
biuret + CO2
-
herterocyclic ring cleavage
-
-
?
cyanuric acid + H2O
biuret + CO2
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
-
-
?
additional information
?
-
-
one step in the six-step pathway for metabolism of melamine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Barbituric acid
-
2,4,6-trihydroxypyrimidine, strong competitive inhibitor, Ki less than 0.0001 mM
Co2+
-
strain A, 1 mM: slight inhibition
Cu2+
-
strain A, 1 mM: slight inhibition
Fe2+
-
strain A, 1 mM: slight inhibition
Zn2+
-
strain A, 1 mM: 100fold inhibition
additional information
-
strain A, no inhibition by 1 mM EDTA, 1 mM Mg2+ or 1 mM Mn2+ as either sulfate or chloride salts
-
additional information
-
strain A, no inhibition by 0.05 mM of ammeline, 2,4-diamino-6-hydroxy-s-triazine, ammelide, 2-amino-4,6-dihydroxy-s-triazine, uracil, 2,4-dihydroxypyrimidine, 5,6-dihydrouracil, cytosine, 4-amino-2-hydroxypyrimidine, 2,4,5-trihydroxypyrimidine
-
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Cook, A.M.; Beilstein, P.; Grossenbacher, H.; Hutter, R.
Ring cleavage and degradative pathway of cyanuric acid in bacteria
Biochem. J.
231
25-30
1985
Klebsiella pneumoniae, Klebsiella pneumoniae 99, Pseudomonas sp.
brenda
Karns, J.S.
Gene sequence and properties of an s-triazine ring-cleavage enzyme from Pseudomonas sp. strain NRRLB-12227
Appl. Environ. Microbiol.
65
3512-3517
1999
Pseudomonas sp., Pseudomonas sp. A
brenda
Eaton, R.W.; Karns, J.S.
Cloning and comparison of the DNA encoding ammelide aminohydrolase and cyanuric acid amidohydrolase from three s-triazine-degrading bacterial strains
J. Bacteriol.
173
1363-1366
1991
Klebsiella pneumoniae, Klebsiella pneumoniae 99, Pseudomonas sp.
brenda
Eaton, R.W.; Karns, J.S.
Cloning and analysis of s-triazine catabolic genes from Pseudomonas sp. strain NRRLB-12227
J. Bacteriol.
173
1215-1222
1991
Pseudomonas sp., Pseudomonas sp. A
brenda
Cook, A.M.
Biodegradation of s-triazine xenobiotics
FEMS Microbiol. Rev.
46
93-116
1987
Gordonia rubripertincta, Gordonia rubripertincta DSM 10347 / NRRLB-15444R, Hormodendrum sp., Klebsiella pneumoniae, Klebsiella pneumoniae 90, Klebsiella pneumoniae 99, Penicillium spp., Pseudomonas sp., Sporothrix schenckii
-
brenda
Seffernick, J.L.; Erickson, J.S.; Cameron, S.; Cho, S.; Dodge, A.G.; Richman, J.; Sadowsky, M.J.; Wackett, L.P.
Defining the cyanuric acid hydrolase (AtzD)/barbiturase protein family: Sequences and Reactions
J. Bacteriol.
194
4579-4588
2012
Acidovorax citrulli, Acidovorax citrulli 12227, Moorella thermoacetica, Pseudomonas sp. (P58329)
brenda
Peat, T.S.; Balotra, S.; Wilding, M.; French, N.G.; Briggs, L.J.; Panjikar, S., Cowieson, N.; Newman, J.; Scott, C.
Cyanuric acid hydrolase: evolutionary innovation by structural concatenation
Mol. Microbiol.
88
1149-1163
2013
Pseudomonas sp. (P58329)
brenda
Yeom, S.; Mutlu, B.; Aksan, A.; Wackett, L.
Bacterial cyanuric acid hydrolase for water treatment
Appl. Environ. Microbiol.
81
6660-6668
2015
Acidovorax citrulli, Acidovorax citrulli 12227, Moorella thermoacetica (Q2RGM7), Pseudomonas sp. (P58329)
-
brenda