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Literature summary for 3.5.2.15 extracted from

  • Karns, J.S.
    Gene sequence and properties of an s-triazine ring-cleavage enzyme from Pseudomonas sp. strain NRRLB-12227 (1999), Appl. Environ. Microbiol., 65, 3512-3517.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.15

Application

Application Comment Organism
agriculture cleavage of the s-triazine ring is an important step in the mineralization of s-triazine compounds, extensive used as herbicides or pesticides in agriculture worldwide, and hence in their complete removal from environment Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
strain A, expression and overproduction in Escherichia coli DH5-alpha, BRL Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Barbituric acid 2,4,6-trihydroxypyrimidine, strong competitive inhibitor, Ki less than 0.0001 mM Pseudomonas sp.
Co2+ strain A, 1 mM: slight inhibition Pseudomonas sp.
Cu2+ strain A, 1 mM: slight inhibition Pseudomonas sp.
Fe2+ strain A, 1 mM: slight inhibition Pseudomonas sp.
additional information strain A, no inhibition by 0.05 mM of ammeline, 2,4-diamino-6-hydroxy-s-triazine, ammelide, 2-amino-4,6-dihydroxy-s-triazine, uracil, 2,4-dihydroxypyrimidine, 5,6-dihydrouracil, cytosine, 4-amino-2-hydroxypyrimidine, 2,4,5-trihydroxypyrimidine; strain A, no inhibition by 1 mM EDTA, 1 mM Mg2+ or 1 mM Mn2+ as either sulfate or chloride salts Pseudomonas sp.
Zn2+ strain A, 1 mM: 100fold inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information strain A, kinetic analyses and kinetic data Pseudomonas sp.
0.05
-
 cyanuric acid strain A, pH 8.0 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information strain A, divalent cations not required, although the possibility that a tightly bound metal ion is present in enzyme cannot be ruled out, no metal analysis of enzyme is performed directly Pseudomonas sp.
additional information strain A, no effect, no activation by 1 mM Mg2+ or 1 mM Mn2+ as either sulfate or chloride salts Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39400
-
 strain A, 4 * 39400, calculated from DNA sequence Pseudomonas sp.
40000
-
 strain A, 4 * 40000, SDS-PAGE Pseudomonas sp.
185000
-
 strain A, SW300 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyanuric acid + H2O Pseudomonas sp. 2,4,6-trihydroxy-1,3,5-triazine biuret + CO2
-
 ?
cyanuric acid + H2O Pseudomonas sp. 1,3,5-triazine-2,4,6(1H,3H,5H)-trione biuret + CO2
-
 ?
cyanuric acid + H2O Pseudomonas sp. 2,4,6-trihydroxy-s-triazine biuret + CO2
-
 ?
cyanuric acid + H2O Pseudomonas sp. first enzyme of degradative pathway of cyanuric acid biuret + CO2
-
 ?
cyanuric acid + H2O Pseudomonas sp. involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria biuret + CO2
-
 ?
cyanuric acid + H2O Pseudomonas sp. herterocyclic ring cleavage biuret + CO2
-
 ?
cyanuric acid + H2O Pseudomonas sp. involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine biuret + CO2
-
 ?
additional information Pseudomonas sp. involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria ?
-
 ?
additional information Pseudomonas sp. involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine ?
-
 ?
additional information Pseudomonas sp. A involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria ?
-
 ?
additional information Pseudomonas sp. A involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 NRRLB-12227
-
Pseudomonas sp. A
-
 NRRLB-12227
-

Purification (Commentary)

Purification (Comment) Organism
strain A, NRRLB-12227, from recombinant Escherichia coli DH5-alpha containing the cloned trzD gene Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
109.5
-
 strain A, from recombinant Escherichia coli containing trzD gene Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanuric acid + H2O high substrate specificity Pseudomonas sp. biuret + CO2
-
 ?
cyanuric acid + H2O 2,4,6-trihydroxy-1,3,5-triazine Pseudomonas sp. biuret + CO2
-
 ?
cyanuric acid + H2O 1,3,5-triazine-2,4,6(1H,3H,5H)-trione Pseudomonas sp. biuret + CO2
-
 ?
cyanuric acid + H2O 2,4,6-trihydroxy-s-triazine Pseudomonas sp. biuret + CO2
-
 ?
cyanuric acid + H2O heterocyclic ring cleavage Pseudomonas sp. biuret + CO2
-
 ?
cyanuric acid + H2O first enzyme of degradative pathway of cyanuric acid Pseudomonas sp. biuret + CO2
-
 ?
cyanuric acid + H2O involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. biuret + CO2
-
 ?
cyanuric acid + H2O herterocyclic ring cleavage Pseudomonas sp. biuret + CO2
-
 ?
cyanuric acid + H2O involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. biuret + CO2
-
 ?
cyanuric acid + H2O high substrate specificity Pseudomonas sp. A biuret + CO2
-
 ?
additional information strain A, no activity with ammeline, 2,4-diamino-6-hydroxy-s-triazine, ammelide, 2-amino-4,6-dihydroxy-s-triazine, uracil, 2,4-dihydroxypyrimidine, 5,6-dihydrouracil, cytosine, 4-amino-2-hydroxypyrimidine, 2,4,5-trihydroxypyrimidine and barbituric acid, 2,4,6-trihydroxypyrimidine Pseudomonas sp. ?
-
 ?
additional information strain A, high substrate specificity, no activity with any other s-triazine or hydroxypyrimidine compound Pseudomonas sp. ?
-
 ?
additional information involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. ?
-
 ?
additional information involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. ?
-
 ?
additional information strain A, no activity with ammeline, 2,4-diamino-6-hydroxy-s-triazine, ammelide, 2-amino-4,6-dihydroxy-s-triazine, uracil, 2,4-dihydroxypyrimidine, 5,6-dihydrouracil, cytosine, 4-amino-2-hydroxypyrimidine, 2,4,5-trihydroxypyrimidine and barbituric acid, 2,4,6-trihydroxypyrimidine Pseudomonas sp. A ?
-
 ?
additional information strain A, high substrate specificity, no activity with any other s-triazine or hydroxypyrimidine compound Pseudomonas sp. A ?
-
 ?
additional information involved in a pathway by which herbicides or pesticides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. A ?
-
 ?
additional information involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. A ?
-
 ?

Subunits

Subunits Comment Organism
tetramer strain A, 4 * 40000, SDS-PAGE Pseudomonas sp.
tetramer strain A, 4 * 39400, calculated from DNA sequence Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 strain A Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
250
-
 cyanuric acid strain A, pH 8.0 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 strain A Pseudomonas sp.