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Information on EC 3.5.1.111 - 2-oxoglutaramate amidase for references in articles please use BRENDA:EC3.5.1.111Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Paenarthrobacter nicotinovorans
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2-oxoglutaramate amidase
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2-oxoglutaramate + H2O = 2-oxoglutarate + NH3
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nicotine degradation I (pyridine pathway)
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S-methyl-5-thio-alpha-D-ribose 1-phosphate degradation I
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Alanine, aspartate and glutamate metabolism
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5-amino-2,5-dioxopentanoate amidohydrolase
The enzyme, which is highly specific for its substrate, participates in the nicotine degradation pathway of several Gram-positive bacteria.
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UniProt
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2-oxoglutaramate + H2O
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L-glutamic acid + H2O
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poor substrate
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6.3
2-Oxoglutaramate
pH 7.1, 30 °C
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194
2-Oxoglutaramate
pH 7.1, 30 °C
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32300
calculated from cDNA
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homotetramer
4 * 32300, gel filtration
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expressed in Escherichia coli
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Cobzaru, C.; Ganas, P.; Mihasan, M.; Schleberger, P.; Brandsch, R.
Homologous gene clusters of nicotine catabolism, including a new omega-amidase for alpha-ketoglutaramate, in species of three genera of Gram-positive bacteria
Res. Microbiol.
162
285-291
2011
Paenarthrobacter nicotinovorans (Q93NG1)
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