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Information on EC 3.5.1.105 - chitin disaccharide deacetylase and Organism(s) Vibrio alginolyticus and UniProt Accession Q99PX1

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IUBMB Comments
Chitin oligosaccharide deacetylase is a key enzyme in the chitin catabolic cascade of chitinolytic Vibrio strains. Besides being a nutrient, the heterodisaccharide product 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine is a unique inducer of chitinase production in Vibrio parahemolyticus . In contrast to EC 3.5.1.41 (chitin deacetylase) this enzyme is specific for the chitin disaccharide [1,3]. It also deacetylates the chitin trisaccharide with lower efficiency . No activity with higher polymers of GlcNAc [1,3].
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Vibrio alginolyticus
UNIPROT: Q99PX1
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The taxonomic range for the selected organisms is: Vibrio alginolyticus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
diacetylchitobiose deacetylase, chitin oligosaccharide deacetylase, vp-cod, pa-cod, n,n'-diacetylchitobiose deacetylase, chitooligosaccharide deacetylase, deacetylase da1, ydjc deacetylase, chitooligosaccharide deacetylase homolog, ce family 4 cod, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CE family 4 COD
-
-
chitin oligosaccharide deacetylase
-
-
SYSTEMATIC NAME
IUBMB Comments
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-D-glucopyranose acetylhydrolase
Chitin oligosaccharide deacetylase is a key enzyme in the chitin catabolic cascade of chitinolytic Vibrio strains. Besides being a nutrient, the heterodisaccharide product 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine is a unique inducer of chitinase production in Vibrio parahemolyticus [2]. In contrast to EC 3.5.1.41 (chitin deacetylase) this enzyme is specific for the chitin disaccharide [1,3]. It also deacetylates the chitin trisaccharide with lower efficiency [3]. No activity with higher polymers of GlcNAc [1,3].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcNAc-beta-(1-4)-GlcN + acetate
show the reaction diagram
i.e. 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-D-glucopyranose. No activity with chitotriose, chitotetraose, chitopentaose and chitohexaose. The 2-acetamido group is completely hydrolyzed within 3 h, no hydrolysis of 2'-acetamide group occurs
i.e. 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine
-
?
GlcNAc-beta-(1,4)-GlcNAc + H2O
GlcNAc-beta-(1,4)-GlcN + acetate
show the reaction diagram
-
i.e. N,N’-diacetylchitobiose
-
-
?
GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcNAc-beta-(1-4)-GlcN + acetate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GlcNAc-beta-(1,4)-GlcNAc + H2O
GlcNAc-beta-(1,4)-GlcN + acetate
show the reaction diagram
-
i.e. N,N’-diacetylchitobiose
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
1 mM AgNO3, complete inhibition
Al3+
1 mM AlCl3, 30% inhibition
Cu2+
1 mM CuCl2, 53% inhibition
Fe2+
1 mM FeSO4, 26% inhibition
Hg2+
1 mM HgCl2, complete inhibition
Sn2+
1 mM SnCl2, 44% inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 11
pH 6.5: about 45% of maximal activity, pH 11: about 55% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
about 50% of maximal activity at 30°C and 50°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.3
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DEAA_VIBAL
427
0
47050
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44700
x * 44700, calculated from sequence
48000
x * 48000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
37°C, 20 min, about 75% loss of activity
696801
5
37°C, 20 min, about 40% loss of activity
696801
7 - 11
37°C, 20 min, stable
696801
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
pH 7.5, 30 min, about 10% loss of activity
50
pH 7.5, 30 min, about 40% loss of activity
70
pH 7.5, 30 min, about 80% loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the COD activity in the culture fluid to which GlcNAc-GlcN is added is higher than that to which (GlcNAc)2 is added
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ohishi, K.; Yamagishi, M.; Ohta, T.; Motosugi, M.; Izumida, H.; Sano, H.; Adachi, K.; Miwa, T.
Purification and properties of two deacetylases produced by Vibrio alginolyticus H-8
Biosci. Biotechnol. Biochem.
61
1113-1117
1997
Vibrio alginolyticus (Q99PX1), Vibrio alginolyticus H-8 (Q99PX1)
-
Manually annotated by BRENDA team
Hirano, T.; Kadokura, K.; Ikegami, T.; Shigeta, Y.; Kumaki, Y.; Hakamata, W.; Oku, T.; Nishio, T.
Heterodisaccharide 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine is a specific inducer of chitinolytic enzyme production in Vibrios harboring chitin oligosaccharide deacetylase genes
Glycobiology
19
1046-1053
2009
no activity in Vibrio furnissii, no activity in Vibrio nereis, no activity in Vibrio orientalis, Vibrio alginolyticus, Vibrio campbellii, Vibrio cholerae serotype O1, Vibrio cholerae serotype O1 RIMD2203102, Vibrio parahaemolyticus, Vibrio parahaemolyticus (A6P4T5), Vibrio parahaemolyticus KN1699, Vibrio parahaemolyticus RIMD2210633, Vibrio proteolyticus
Manually annotated by BRENDA team
Ohishi, K.; Murase, K.; Ohta, T.; Etoh, H.
Cloning and sequencing of the deacetylase gene from Vibrio alginolyticus H-8
J. Biosci. Bioeng.
90
561-563
2000
Vibrio alginolyticus (Q99PX1), Vibrio alginolyticus H-8 (Q99PX1), Vibrio alginolyticus H-8
Manually annotated by BRENDA team
Hirano, T.; Aoki, M.; Kadokura, K.; Kumaki, Y.; Hakamata, W.; Oku, T.; Nishio, T.
Heterodisaccharide 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine is an effective chemotactic attractant for Vibrio bacteria that produce chitin oligosaccharide deacetylase
Lett. Appl. Microbiol.
53
161-166
2011
no activity in Vibrio furnissii, no activity in Vibrio furnissii RIMD2223001, no activity in Vibrio nereis, no activity in Vibrio nereis NBRC15637, Vibrio alginolyticus, Vibrio alginolyticus NBRC15630, Vibrio parahaemolyticus, Vibrio parahaemolyticus KN1699, Vibrio parahaemolyticus RIMD2210633
Manually annotated by BRENDA team