EC Number   |
|---|
   3.5.1.105 | - |
| 3.5.1.105 | expression in Escherichia coli |
| 3.5.1.105 | expression of the soluble recombinant protein in Escherichia coli |
| 3.5.1.105 | gene chbG or ydjC, encoded in the chb operon, DNA and amino acid sequence determination and analysis |
| 3.5.1.105 | gene cod, recombinant expression of StrepII-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) |
| 3.5.1.105 | gene nodB, recombinant expression of StrepII-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 3.5.1.105 | gene PH0499, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) and in Bacillus subtilis strain WB600, subcloning in Escherichia coli strain JM109, method optimization, overview |
| 3.5.1.105 | gene PH0499, recombinant expression of wild-type and mutants in Bacillus subtilis strain WB600 under control of the HpaII promoter and secreteion as extracellular enzymes, screening of signal peptides from Bacillus subtilis for optimal expression. The signal peptide YncM achieves the highest extracellular diacetylchitobiose deacetylase activity of 13.5 U/ml. Subcloning in Escherichia coli. Quantitative real-time PCR expression analysis. Method optimization, overview |
| 3.5.1.105 | gene Sbal_1411, cloning in Escherichia coli strain DH5alpha and recombinant expression in Escherichia coli strain HMS174(DE3), the recombinant protein is secreted to the culture medium |
| 3.5.1.105 | gene VP2638, quantitative real-time PCR expression analysis |
