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Information on EC 3.4.24.45 - atrolysin F for references in articles please use BRENDA:EC3.4.24.45Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Crotalus atrox
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Cleavage of Val2-/-Asn, Gln4-/-His, Leu6-/-Cys, His10-/-Leu, Ala14-/-Leu and Tyr16-/-Leu bonds in insulin B chain
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hydrolysis of peptide bond
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Crotalus atrox metalloendopeptidase f
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Hemorrhagic toxin f
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brenda
Western diamondback rattlesnake
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brenda
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Basement membrane preparation + H2O
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Basement membranes surrounding capillaries + H2O
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Collagen IV + H2O
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basement membrane component
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Fibrinogen + H2O
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Fibrinogen + H2O
Hydrolyzed fibrinogen
Fibronectin + H2O
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Gelatin of collagen type I + H2O
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Gelatin of collagen type III + H2O
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Gelatin of collagen type IV + H2O
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Laminin + H2O
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basement membrane component
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Nidogen + H2O
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basement membrane component
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Oxidized insulin B-chain + H2O
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Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
additional information
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Basement membranes surrounding capillaries + H2O
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involved in disruption of capillary membranes
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Basement membranes surrounding capillaries + H2O
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produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver
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Basement membranes surrounding capillaries + H2O
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together with atrolysin A member of the class P-III hemorrhagic toxins
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Dimethylcasein + H2O
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Dimethylcasein + H2O
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Fibrinogen + H2O
Hydrolyzed fibrinogen
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no coagulation
non-clottable products
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Fibrinogen + H2O
Hydrolyzed fibrinogen
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cleaves gamma-chain, Aalpha and Bbeta-chains remain intact
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Fibrinogen + H2O
Hydrolyzed fibrinogen
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cleaves gamma-chain, Aalpha and Bbeta-chains remain intact
non-clottable products
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Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
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Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
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cleaves Val2-Asn3, Gln4-His5, Leu6-Cys7, H10-Leu11, Ala14-Leu15, Tyr16-Leu17 bonds
several peptides
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additional information
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additional information
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no hydrolysis of tosyl-L-Arg methyl ester or N-benzoyl-L-Arg ethyl ester
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Basement membranes surrounding capillaries + H2O
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Basement membranes surrounding capillaries + H2O
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involved in disruption of capillary membranes
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Basement membranes surrounding capillaries + H2O
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produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver
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Basement membranes surrounding capillaries + H2O
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together with atrolysin A member of the class P-III hemorrhagic toxins
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Zn2+
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zinc metalloproteinase, 1 mol zinc/mol enzyme
Zn2+
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essential for hemorrhagic and proteolytic activity; zinc metalloproteinase, 1 mol zinc/mol enzyme
Zn2+
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1.15 mol per mol enzyme
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1,10-phenanthroline
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1,10-phenanthroline
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casein as substrate
1,10-phenanthroline
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inhibits proteolytic activity
EDTA
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EDTA
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casein as substrate
EDTA
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inhibits proteolytic activity
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additional information
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pI: 7.7
additional information
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pI: 7.7
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brenda
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additional information
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amino acid composition, immunologically not related to hemorrhagic toxin a or c
64000
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1 * 64000, Crotalus atrox, SDS-PAGE
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monomer
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1 * 64000, Crotalus atrox, SDS-PAGE
monomer
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1 * 64000, Crotalus atrox, SDS-PAGE; in the presence of 2-mercaptoethanol
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from lyophilized crude venom
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ion-exchange chromatography and gel filtration
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medicine
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second-most potent hemorrhagic toxin of all the atrolysins, systemic hemorrhage in lung, kidney, liver, heart, destruction of muscle tissue
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Fox, J.W.; Bjarnason, J.B.
Atrolysins: metalloproteinases from Crotalus atrox venom
Methods Enzymol.
248
368-387
1995
Crotalus atrox
brenda
Nikai, T.; Mori, N.; Kishida, M.; Sugihara, H.; Tu, A.T.
Isolation and biochemical characterization of hemorrhagic toxin f from the venom of Crotalus atrox (western diamondback rattlesnake)
Arch. Biochem. Biophys.
231
309-319
1984
Crotalus atrox
brenda
Fox, J.W.; Bjarnason, J.B.
Atrolysin F
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
676-677
2004
Crotalus atrox
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brenda
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