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Information on EC 3.4.21.100 - sedolisin and Organism(s) Pseudomonas sp. and UniProt Accession P42790

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.100 sedolisin
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Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas sp.
UNIPROT: P42790 not found.
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Word Map
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The taxonomic range for the selected organisms is: Pseudomonas sp.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
hide
Hydrolysis of the B chain of insulin at -Glu13-/-Ala-, -Leu15-/-Tyr- and -Phe25-/-Tyr-, and angiotensin I at -Tyr4-/-Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe-/-Phe(NO2)-Arg-Leu
Synonyms
sedolisin, pepstatin-insensitive carboxyl proteinase, grifolisin, scytalidolisin, pseudomonas serine-carboxyl proteinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Pepstatin-insensitive carboxyl proteinase
-
-
-
-
pseudomonalisin
-
-
-
-
pseudomonapepsin
Pseudomonas serine-carboxyl proteinase
-
-
Pseudomonas sp. pepstatin-insensitive carboxyl proteinase
-
-
-
-
sedolisin
CAS REGISTRY NUMBER
COMMENTARY hide
848318-58-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Leu-Leu-Glu-Phe-Leu + H2O
Leu-Leu-Glu + Phe-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
?
show the reaction diagram
-
-
?
Acid-denatured hemoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Asp-Phe + 4-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
-
?
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ala-Lys-Leu-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Leu + 4-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
-
?
Lys-Pro-Ala-Lys-Phe-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Phe + 4-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
-
?
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Arg-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Asn-Gln-Phe-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asn-Gln-Phe + 4-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
-
?
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asn-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asp-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Gln-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Glu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Arg-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asn-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asp-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Gln-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ala-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ala
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Arg
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Asp
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ile
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Phe
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ser
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Tyr
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Val
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asn-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asp-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Glu-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ile-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Leu-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Lys-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ser-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Val-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-His-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ile-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Leu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Lys-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Nle-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ser-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Thr-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Val-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Leu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Lys-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ser-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Thr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Val-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Val-Ser-Tyr-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Val-Ser-Tyr + 4-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
-
?
Mca-Lys-Pro-Pro-Leu-Glu-Tyr-Arg-Leu-Gly-Lys(DNP)-Gly + H2O
Mca-Lys-Pro-Pro-Leu-Glu + Tyr-Arg-Leu-Gly-Lys(DNP)-Gly
show the reaction diagram
-
-
-
?
Ser-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu + H2O
Ser-Pro-Ala-Lys-Phe + (4-nitro)Phe-Arg-Leu
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
necessary for the activity of the enzyme
Ca2+
-
Ca2+-binding site
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetyl-Ile-Ala-Phe
-
acetyl-Ile-Pro-Phe
-
chymostatin
-
iodotyrosine
-
iodotyrostatin
-
pseudo-iodotyrostatin
-
pseudo-tyrostatin
-
pseudoiodotyrostatin
-
1,2-epoxy-3-(4-nitrophenoxy)propane
-
modifies the enzyme with concomitant loss of activity
HgCl2
-
slight
N,N'-dicyclohexylcarbodiimide
-
inhibitor binds to Asp140 and Glu222 of the enzyme, inactivation with pseudo-first-order kinetics, inactivation is prevented by a competitive inhibitor, tyrostatin
PCMB
-
slight
pseudo-iodotyrostatin
-
two molecules of the inhibitor are bound in the active site of the enzyme
Tyrostatin
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0067 - 0.149
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
0.013
Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu
-
-
-
0.0174
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.06 - 0.095
Lys-Pro-Ala-Lys-Leu-4-nitrophenylalanine-Arg-Leu
-
0.0224
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0247
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0242
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0162
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00612
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0131
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.01
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0189
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0106
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0207
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.003 - 0.052
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu
0.00827
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
-
pH 3.5, 37°C
0.00697
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
-
pH 3.5, 37°C
0.00542
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
-
pH 3.5, 37°C
0.00962
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
-
pH 3.5, 37°C
0.0037
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
-
pH 3.5, 37°C
0.0111
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0074
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
-
pH 3.5, 37°C
0.0101
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
-
pH 3.5, 37°C
0.00627
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
-
pH 3.5, 37°C
0.00547
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
-
pH 3.5, 37°C
0.0309
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
-
pH 3.5, 37°C
0.00946
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
-
pH 3.5, 37°C
0.00901
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
-
pH 3.5, 37°C
0.0153
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
-
pH 3.5, 37°C
0.00567
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
-
pH 3.5, 37°C
0.0189
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
-
pH 3.5, 37°C
0.014
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
-
pH 3.5, 37°C
0.028
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
-
pH 3.5, 37°C
0.0229
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.017
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00422
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0564
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00713
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0275
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00999
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0106
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00782
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00884
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0275
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00875
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0132
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0115
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.019
Lys-Pro-Val-Ser-Tyr-4-nitrophenylalanine-Arg-Leu
-
-
-
0.00012
Mca-Lys-Pro-Pro-Leu-Glu-Tyr-Arg-Leu-Gly-Lys(DNP)-Gly
-
-
additional information
additional information
-
overview: Km of synthetic substrates
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.055 - 65.2
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
6.8
Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu
-
-
-
6 - 57.6
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
14.7
Lys-Pro-Ala-Lys-Phe-4-nitrophenylalanine-Arg-Leu
-
-
-
53.1
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
5.5
Lys-Pro-Asn-Gln-Phe-4-nitrophenylalanine-Arg-Leu
-
-
-
47.1
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.052 - 9.68
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
24.5
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
3.13 - 3.82
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
27.7
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
12.5
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.04 - 8.79
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
20.9
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
10.4
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
6.9
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu
-
-
19
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
-
pH 3.5, 37°C
15.5
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
-
pH 3.5, 37°C
3.13 - 3.64
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
40.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
-
pH 3.5, 37°C
10.9
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
-
pH 3.5, 37°C
3.7 - 32.4
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
10.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
-
pH 3.5, 37°C
44.4
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
-
pH 3.5, 37°C
0.052 - 9.87
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
11.6
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
-
pH 3.5, 37°C
0.5 - 12.9
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
33
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
-
pH 3.5, 37°C
0.017 - 33.3
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
3.3 - 5.24
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
0.031 - 8.38
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
0.64 - 54.1
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
11.8
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
-
pH 3.5, 37°C
3.3 - 5.38
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
2.93 - 6
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
18.3
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
27.2
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
9.75
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.55 - 14.8
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
29.3
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.55 - 14.8
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
22.9
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
15.7
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
3.1
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
1.5 - 56.3
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
20.4
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.031 - 7.92
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
0.55 - 14.8
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
0.0867
Lys-Pro-Val-Ser-Tyr-4-nitrophenylalanine-Arg-Leu
-
-
-
73
Mca-Lys-Pro-Pro-Leu-Glu-Tyr-Arg-Leu-Gly-Lys(DNP)-Gly
-
-
additional information
additional information
-
overview: turnover number of synthetic substrates
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0006
acetyl-Ile-Ala-Phe
-
0.000415
acetyl-Ile-Pro-Phe
-
0.000045
chymostatin
-
0.000032
iodotyrostatin
-
0.000014
pseudo-iodotyrostatin
-
0.000056
pseudo-tyrostatin
-
0.000006
Tyrostatin
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
acid-denatured hemoglobin, casein
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
-
Pseudomonas sp., gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 42000, Pseudomonas sp., SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
N-terminal propeptide
additional information
-
less than 0.05% carbohydrate
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure complexed with a number of inhibitors (tyrostatin, pseudo-tyrostatin, acetyl-Ile-Pro-Phe, acetyl-Ile-Ala-Phe, iodotyrostatin, pseudotyrostatin and chymostatin), have been solved and refined at high-level to atomic-level resolution
crystal structure solved by single-wavelength anomalous diffraction using the absorption peak of bromide anions. Structures of the uninhibited enzyme and of complexes with iodotyrosine are refined at 1.0-1.4 A resolution
high-resolution crystrallographic analysis of a complex of the enzyme with pseudo-iodotyrostatin
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D124A
slightly lower proteinase activity than that of wild-type enzyme
D170A
mutant enzyme is not processed autocatalytically in Escherichia coli
D225A
mutant enzyme shows almost the same proteinase activity as wild-type enzyme
D254A
mutant enzyme shows almost the same proteinase activity as wild-type enzyme
D328A
mutant enzyme is not processed autocatalytically in Escherichia coli
D84A
proteinase activity is 0.2% of that of wild-type enzyme, mutant enzyme is very unstable
E217A
mutant enzyme shows almost the same proteinase activity as wild-type enzyme
E222A
mutant enzyme is not processed autocatalytically in Escherichia coli
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 5.5
-
15 h, 37°C, stable
137232
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
15 min, stable below
65
-
complete loss of activity after 15 min
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant mutant enzymes D124A, D225A, D254A, E217A, D84A, D170A, D328A and E222A
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Oda, K.; Fukuda, Y.; Murao, S.; Uchida, K.; Kainosho, M.
Novel proteinase inhibitor, tyrostatin, inhibiting some pepstatin-insensitive carboxyl proteinases
Agric. Biol. Chem.
53
405-415
1989
Pseudomonas sp., Pseudomonas sp. No. 101, Xanthomonas sp., Xanthomonas sp. No. T-22
-
Manually annotated by BRENDA team
Oda, K.; Sugitani, M.; Fukuhara, K.; Murao, S.
Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative bacterium
Biochim. Biophys. Acta
923
463-469
1987
Pseudomonas sp., Pseudomonas sp. No. 101
Manually annotated by BRENDA team
Oda, K.; Nakatani, H.; Dunn, B.M.
Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101
Biochim. Biophys. Acta
1120
208-214
1992
Pseudomonas sp., Pseudomonas sp. No. 101
Manually annotated by BRENDA team
Wlodawer, A.; Li, M.; Gustchina, A.; Oyama, H.; Dunn, B.M.; Oda, K.
Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases
Acta Biochim. Pol.
50
81-102
2003
Pseudomonas sp.
Manually annotated by BRENDA team
Wlodawer, A.; Li, M.; Gustchina, A.; Oyama, H.; Oda, K.; Beyer, B.B.; Clemente, J.; Dunn, B.M.
Two inhibitor molecules bound in the active site of Pseudomonas sedolisin: a model for the bi-product complex following cleavage of a peptide substrate
Biochem. Biophys. Res. Commun.
314
638-645
2004
Pseudomonas sp.
Manually annotated by BRENDA team
Wlodawer, A.; Li, M.; Gustchina, A.; Dauter, Z.; Uchida, K.; Oyama, H.; Goldfarb, N.E.; Dunn, B.M.; Oda, K.
Inhibitor complexes of the Pseudomonas serine-carboxyl proteinase
Biochemistry
40
15602-15611
2001
Pseudomonas sp. (P42790), Pseudomonas sp.
Manually annotated by BRENDA team
Ito, M.; Narutaki, S.; Uchida, K.; Oda, K.
Identification of carboxyl residues in pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. 101 that participate in catalysis and substrate binding
J. Biochem.
125
210-216
1999
Pseudomonas sp.
Manually annotated by BRENDA team
Narutaki, S.; Dunn, B.M.; Oda, K.
Subsite preferences of pepstatin-insensitive carboxyl proteinases from bacteria
J. Biochem.
125
75-81
1999
Pseudomonas sp.
Manually annotated by BRENDA team
Oyama, H.; Abe, S.I.; Ushiyama, S.; Takahashi, S.; Oda, K.
Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis
J. Biol. Chem.
274
27815-27822
1999
Pseudomonas sp. (P42790)
Manually annotated by BRENDA team
Wlodawer, A.; Li, M.; Dauter, Z.; Gustchina, A.; Uchida, K.; Oyama, H.; Dunn, B.M.; Oda, K.
Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes
Nat. Struct. Biol.
8
442-446
2001
Pseudomonas sp. (P42790), Pseudomonas sp.
Manually annotated by BRENDA team
Xu, Q.; Yao, J.; Wlodawer, A.; Guo, H.
Clarification of the mechanism of acylation reaction and origin of substrate specificity of the serine-carboxyl peptidase sedolisin through QM/MM free energy simulations
J. Phys. Chem. B
115
2470-2476
2011
Pseudomonas sp. (P42790)
Manually annotated by BRENDA team