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Literature summary for 3.4.21.100 extracted from

  • Oda, K.; Sugitani, M.; Fukuhara, K.; Murao, S.
    Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative bacterium (1987), Biochim. Biophys. Acta, 923, 463-469.
    View publication on PubMed
Search for more literature for 3.4.21.100

Inhibitors

Inhibitors Comment Organism Structure
1,2-epoxy-3-(4-nitrophenoxy)propane modifies the enzyme with concomitant loss of activity Pseudomonas sp.
HgCl2 slight Pseudomonas sp.
additional information diazoacetyl-DL-norleucine methyl ester; diisopropyl fluorophosphate (1 mM); EDTA (1 mM); iodoacetic acid (1 mM); not: pepstatin; p-chloromercuribenzenesulfonic acid; phenyl methane sulfonyl fluoride (1 mM); Streptomyces pepsin inhibitor Pseudomonas sp.
PCMB slight Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 Pseudomonas sp., gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 No. 101
-
Pseudomonas sp. No. 101
-
 No. 101
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information less than 0.05% carbohydrate Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acid-denatured hemoglobin + H2O
-
 Pseudomonas sp. ?
-
 ?
Acid-denatured hemoglobin + H2O
-
 Pseudomonas sp. No. 101 ?
-
 ?
casein + H2O
-
 Pseudomonas sp. ?
-
 ?
casein + H2O
-
 Pseudomonas sp. No. 101 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, Pseudomonas sp., SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 15 min, stable below Pseudomonas sp.
65
-
 complete loss of activity after 15 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 acid-denatured hemoglobin, casein Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5.5 15 h, 37°C, stable Pseudomonas sp.