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Literature summary for 3.4.21.100 extracted from

  • Oyama, H.; Abe, S.I.; Ushiyama, S.; Takahashi, S.; Oda, K.
    Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis (1999), J. Biol. Chem., 274, 27815-27822.
    View publication on PubMed
Search for more literature for 3.4.21.100

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
D124A slightly lower proteinase activity than that of wild-type enzyme Pseudomonas sp.
D170A mutant enzyme is not processed autocatalytically in Escherichia coli Pseudomonas sp.
D225A mutant enzyme shows almost the same proteinase activity as wild-type enzyme Pseudomonas sp.
D254A mutant enzyme shows almost the same proteinase activity as wild-type enzyme Pseudomonas sp.
D328A mutant enzyme is not processed autocatalytically in Escherichia coli Pseudomonas sp.
D84A proteinase activity is 0.2% of that of wild-type enzyme, mutant enzyme is very unstable Pseudomonas sp.
E217A mutant enzyme shows almost the same proteinase activity as wild-type enzyme Pseudomonas sp.
E222A mutant enzyme is not processed autocatalytically in Escherichia coli Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme D124A Pseudomonas sp.
0.0074
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, wild-type enzyme Pseudomonas sp.
0.0091
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme D265A Pseudomonas sp.
0.0101
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme E217A Pseudomonas sp.
0.0296
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme D225A Pseudomonas sp.
0.149
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme D84A Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. P42790
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant enzymes D124A, D225A, D254A, E217A, D84A, D170A, D328A and E222A Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
 Pseudomonas sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
PCP
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.055
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme D84A Pseudomonas sp.
7
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, wild-type enzyme Pseudomonas sp.
39
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme D124A Pseudomonas sp.
56
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme E217A Pseudomonas sp.
59.3
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, wild-type enzyme Pseudomonas sp.
59.5
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme D265A Pseudomonas sp.
65.2
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme D225A Pseudomonas sp.