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Information on EC 3.4.21.100 - sedolisin
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3.4.21.100 sedolisinSpecify your search results
Word Map
- 3.4.21.100
- peptidase
- proteinases
- subtilisins
- merops
-
ceroid
- pcp
- lipofuscinosis
-
tripeptidyl-peptidase
- diazoacetyl-dl-norleucine
-
subtilisin-like
- prosegment
-
tripeptidyl
- b-chain
-
1,2-epoxy-3-p-nitrophenoxypropane
-
coagulans
- medicine
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Hydrolysis of the B chain of insulin at -Glu13-/-Ala-, -Leu15-/-Tyr- and -Phe25-/-Tyr-, and angiotensin I at -Tyr4-/-Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe-/-Phe(NO2)-Arg-Leu
Synonyms
sedolisin, pepstatin-insensitive carboxyl proteinase, grifolisin, scytalidolisin, pseudomonas serine-carboxyl proteinase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
EC 3.4.23.37
-
-
formerly
-
PCP
Pepstatin-insensitive carboxyl proteinase
-
-
-
-
PSCP
pseudomonalisin
-
-
-
-
pseudomonapepsin
Pseudomonas sp. pepstatin-insensitive carboxyl proteinase
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-
-
-
SedA
SedB
SedC
SedD
sedolisin
pseudomonapepsin
-
-
-
-
sedolisin
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of the B chain of insulin at -Glu13-/-Ala-, -Leu15-/-Tyr- and -Phe25-/-Tyr-, and angiotensin I at -Tyr4-/-Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe-/-Phe(NO2)-Arg-Leu
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
serine endopeptidase
-
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CAS REGISTRY NUMBER
COMMENTARY
848318-58-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O
Arg-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe
Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Asp-Phe + 4-nitrophenylalanine-Arg-Leu
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ala-Lys-Leu-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Leu + 4-nitrophenylalanine-Arg-Leu
Lys-Pro-Ala-Lys-Phe-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Phe + 4-nitrophenylalanine-Arg-Leu
-
-
-
-
?
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Arg-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Asn-Gln-Phe-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asn-Gln-Phe + 4-nitrophenylalanine-Arg-Leu
-
-
-
-
?
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asn-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asp-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Gln-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Glu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Arg-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asn-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asp-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Gln-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ala-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ala
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Arg
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Asp
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ile
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Phe
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ser
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Tyr
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Val
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asn-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asp-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Glu-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ile-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Leu-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Lys-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ser-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Val-Leu
-
-
-
?
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-His-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ile-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Leu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Lys-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Nle-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ser-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Thr-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Val-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Leu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Lys-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ser-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Thr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Val-Glu-Phe + p-nitrophenylalanine-Arg-Leu
-
-
-
?
Lys-Pro-Val-Ser-Tyr-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Val-Ser-Tyr + 4-nitrophenylalanine-Arg-Leu
-
-
-
-
?
Mca-Lys-Pro-Pro-Leu-Glu-Tyr-Arg-Leu-Gly-Lys(DNP)-Gly + H2O
Mca-Lys-Pro-Pro-Leu-Glu + Tyr-Arg-Leu-Gly-Lys(DNP)-Gly
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-
-
?
Oxidized insulin B-chain + H2O
?
-
hydrolysis of four bonds, F1-V2, A14-L15, G20-E21, F24-F25
-
-
?
Ala-Ala-Phe-p-nitroanilide + H2O
Ala-Ala-Phe + p-nitroaniline
-
SedB, SedC, and SedD
-
-
?
Ala-Ala-Phe-p-nitroanilide + H2O
Ala-Ala-Phe + p-nitroaniline
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SedB, SedC, and SedD
-
-
?
Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O
Arg-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe
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SedB
-
-
?
Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O
Arg-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe
-
SedB
-
-
?
Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Asp-Phe + 4-nitrophenylalanine-Arg-Leu
-
-
-
-
?
Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Asp-Phe + 4-nitrophenylalanine-Arg-Leu
-
-
-
-
?
Lys-Pro-Ala-Lys-Leu-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Leu + 4-nitrophenylalanine-Arg-Leu
-
-
-
-
?
Lys-Pro-Ala-Lys-Leu-4-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Leu + 4-nitrophenylalanine-Arg-Leu
-
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
-
-
?
Phe-Pro-Ala-p-nitroanilide + H2O
Phe-Pro-Ala + p-nitroaniline
-
SedB, SedC, and SedD
-
-
?
Phe-Pro-Ala-p-nitroanilide + H2O
Phe-Pro-Ala + p-nitroaniline
-
SedB, SedC, and SedD
-
-
?
Ser-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu + H2O
Ser-Pro-Ala-Lys-Phe + (4-nitro)Phe-Arg-Leu
-
-
-
?
Ser-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu + H2O
Ser-Pro-Ala-Lys-Phe + (4-nitro)Phe-Arg-Leu
-
-
-
?
additional information
?
-
-
SedA is an acidic endoprotease. SedB, SedC, and SedD are tripeptidyl-peptidases with a common specificity for tripeptide-p-nitroanilide substrates at acidic pHs
-
-
?
additional information
?
-
-
SedA is an acidic endoprotease. SedB, SedC, and SedD are tripeptidyl-peptidases with a common specificity for tripeptide-p-nitroanilide substrates at acidic pHs
-
-
?
additional information
?
-
-
substrate specificity against synthetic substrates: synthetic substrates with general structure P5-P4-P3-P2-P1*(4-nitro)Phe-Arg-Leu. The nature of the residue occupying the P2, P3 and P4 position as well as P1 position has strong influence on kinetic parameters
-
-
?
additional information
?
-
-
the best substrate of the type Lys-Pro-Ile(P3)-Glu(P2)-Phe-/-p-nitrophenylalanine-Arg(P2')-Leu(P3') has a Leu at the P2 position
-
?
additional information
?
-
sedolisin shows a relatively high activity on substrates with Glu at P1 site
-
-
?
additional information
?
-
-
substrate specificity against synthetic substrates: synthetic substrates with general structure P5-P4-P3-P2-P1*(4-nitro)Phe-Arg-Leu. The nature of the residue occupying the P2, P3 and P4 position as well as P1 position has strong influence on kinetic parameters
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1,2-epoxy-3-(4-nitrophenoxy)propane
-
modifies the enzyme with concomitant loss of activity
N,N'-dicyclohexylcarbodiimide
-
inhibitor binds to Asp140 and Glu222 of the enzyme, inactivation with pseudo-first-order kinetics, inactivation is prevented by a competitive inhibitor, tyrostatin
pseudo-iodotyrostatin
-
two molecules of the inhibitor are bound in the active site of the enzyme
additional information
-
not inhibitory: pepstatin, 1,10-phenanthroline, EDTA, N-ethylmaleimide
-
additional information
-
diazoacetyl-DL-norleucine methyl ester; diisopropyl fluorophosphate (1 mM); EDTA (1 mM); iodoacetic acid (1 mM); not: pepstatin; p-chloromercuribenzenesulfonic acid; phenyl methane sulfonyl fluoride (1 mM); Streptomyces pepsin inhibitor
-
additional information
-
insensitive to pepstatin, diazoacetyl-DL-norleucine methyl ester and 1,2-epoxy-3(p-nitrophenoxy)propane
-
additional information
-
not inhibited by pepstatin. The hydrophilic nature of the S2' subsite appears to be a distinguishing feature of pepstatin-insensitive carboxyl proteinases
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Lysosomal Storage Diseases
Classical late infantile neuronal ceroid lipofuscinosis fibroblasts are deficient in lysosomal tripeptidyl peptidase I.
Neuronal Ceroid-Lipofuscinoses
Aorsin, a novel serine proteinase with trypsin-like specificity at acidic pH.
Neuronal Ceroid-Lipofuscinoses
Classical late infantile neuronal ceroid lipofuscinosis fibroblasts are deficient in lysosomal tripeptidyl peptidase I.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
overview: Km of synthetic substrates
-
0.0067
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme D124A
0.0074
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, wild-type enzyme
0.0091
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme D265A
0.0101
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E217A
0.0296
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme D225A
0.149
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme D84A
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
overview: turnover number of synthetic substrates
-
0.055
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme D84A
7
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, wild-type enzyme
39
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme D124A
56
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme E217A
59.3
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, wild-type enzyme
59.5
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme D265A
65.2
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
pH 3.5, 37°C, mutant enzyme D225A
0.031 - 0.51