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Information on EC 3.2.1.4 - cellulase and Organism(s) Caldicellulosiruptor saccharolyticus and UniProt Accession P22534

for references in articles please use BRENDA:EC3.2.1.4

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EC Tree

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.4 cellulase

Will also hydrolyse 1,4-linkages in beta-D-glucans also containing 1,3-linkages.

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Caldicellulosiruptor saccharolyticus

UNIPROT: P22534

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The taxonomic range for the selected organisms is: Caldicellulosiruptor saccharolyticus
The enzyme appears in selected viruses and cellular organisms

Reaction Schemes

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Synonyms

cellulase, cel7a, cellobiohydrolase i, cel5a, cel6a, avicelase, endocellulase, celluclast, cel7b, cbhii, show all synonyms

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CelA

Caldicellulosiruptor saccharolyticus

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Csac_1076

Caldicellulosiruptor saccharolyticus

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9.5 cellulase

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Abscission cellulase

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alkali cellulase

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Alkaline cellulase

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avicelase

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beta-1,4-endoglucan hydrolase

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beta-1,4-glucanase

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Carboxymethyl cellulase

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Carboxymethyl-cellulase

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carboxymethylcellulase

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CEL1

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CelB

Caldicellulosiruptor saccharolyticus

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cellobiohydrolase

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celluase A

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celludextrinase

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Cellulase

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cellulase A 3

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Cellulase E1

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Cellulase E2

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Cellulase E4

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Cellulase E5

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Cellulase SS

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Cellulase V1

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cellulosin AP

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CMCase

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Csac_1078

Caldicellulosiruptor saccharolyticus

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CX-cellulase

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EG1

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EG2

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EG3

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EGA

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EGB

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EGC

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EGCCA

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EGCCC

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EGCCD

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EGCCF

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EGCCG

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EGD

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EGE

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EGF

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EGH

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EGI

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EGIV

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EGM

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EGSS

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EGX

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EGY

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EGZ

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endo-1,4-beta-D-glucanase

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endo-1,4-beta-glucanase

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endo-1,4-beta-glucanase E1

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endo-1,4-beta-glucanase V1

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endocellulase E1

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endoglucanase

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endoglucanase D

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FI-CMCASE

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pancellase SS

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Thermoactive cellulase

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hydrolysis of O-glycosyl bond

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BRENDA

cellulose degradation

KEGG

Starch and sucrose metabolism

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4-beta-D-glucan 4-glucanohydrolase

Will also hydrolyse 1,4-linkages in beta-D-glucans also containing 1,3-linkages.

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9012-54-8

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carboxymethylcellulose + H2O

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Caldicellulosiruptor saccharolyticus

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cellulose + H2O

D-glucose + ?

show the reaction diagram

Caldicellulosiruptor saccharolyticus

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enzyme is able to degrade crystalline cellulose to glucose

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carboxymethylcellulose + H2O

additional information

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Caldicellulosiruptor saccharolyticus

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?

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85

Caldicellulosiruptor saccharolyticus

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Caldicellulosiruptor saccharolyticus

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UniProt

Manually annotated by BRENDA team

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GUNA_CALSA

Caldicellulosiruptor saccharolyticus

1742

0

193697

Swiss-Prot

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118000 - 120000

Caldicellulosiruptor saccharolyticus

PAGE

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additional information

Caldicellulosiruptor saccharolyticus

the exoglucanase activity is located in the amino-terminal domain of the enzyme and the endoglucanase activity is located in the carboxy-terminal domain

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additional information

Caldicellulosiruptor saccharolyticus

construction of deletion mutants expressing solely the carboxyterminal domain containing the endoglucanase activity. Temperature optimum and stability of the deletion mutants are the same as wild-type

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70

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Caldicellulosiruptor saccharolyticus

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expression in Thermotoga sp. strain RQ2 and Escherichia coli

Caldicellulosiruptor saccharolyticus

expression in Escherichia coli

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expression in Thermotoga sp. strain RQ2 and Escherichia coli

Caldicellulosiruptor saccharolyticus

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synthesis

Caldicellulosiruptor saccharolyticus

expression of enzyme in Escherichia coli and Thermotoga sp. after fusion to the signal peptides of TM1840 (amyA) or TM0070 (xynB). Expressed in Escherichia coli and Thermotoga sp. renders the hosts with increased endoglucanase activities. In Escherichia coli, the recombinant enzymes are mainly bound to the bacterial cells, whereas in Thermotoga sp., about half of the enzyme activities are observed in the culture supernatants. However, the cellulase activities are lost in Thermotoga sp. after three consecutive transfers

synthesis

Caldicellulosiruptor saccharolyticus

expression of enzyme in Escherichia coli and Thermotoga sp. after fusion to the signal peptides of TM1840 (amyA) or TM0070 (xynB). Expressed in Escherichia coli and Thermotoga sp. renders the hosts with increased endo- and exoglucanase activities. In Escherichia coli, the recombinant enzymes are mainly bound to the bacterial cells, whereas in Thermotoga sp., about half of the enzyme activities are observed in the culture supernatants. However, the cellulase activities are lost in Thermotoga sp. after three consecutive transfers

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Patchett, M.L.; Neal, T.L.; Schofield, L.R.; Strange, R.C.; Daniel, R.M.; Morgan, H.W.

Heat treatment purification of thermostable cellulase and hemicellulase enzymes expressed in E. coli

Enzyme Microb. Technol.

11

113-115

1989

Caldicellulosiruptor saccharolyticus

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Manually annotated by BRENDA team

Saul, D.J.; Williams, L.C.; Grayling, R.A.; Chamley, L.W.; Love, D.R.; Bergquist, P.L.

celB, a gene coding for a bifunctional cellulase from the extreme thermophile "Caldocellum saccharolyticum"

Appl. Environ. Microbiol.

56

3117-3124

1990

Caldicellulosiruptor saccharolyticus (P10474)

Manually annotated by BRENDA team

Xu, H.; Han, D.; Xu, Z.

Expression of heterologous cellulases in Thermotoga sp. strain RQ2

Biomed Res. Int.

2015

304523

2015

Caldicellulosiruptor saccharolyticus (P10474), Caldicellulosiruptor saccharolyticus (P22534), Caldicellulosiruptor saccharolyticus

Manually annotated by BRENDA team

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Transporter Classification Database (TCDB): 4.D.3.1.6

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Release 2023.1 (January 2023)