EC Number |
---|
3.2.1.4 | - |
3.2.1.4 | 12 mutant genes with changes in fice conserved residues of Cel9A-68 are constructed, cloned and expressed in Escherichia coli |
3.2.1.4 | a chimeric xylanase/endoglucanase with an internal cellulase-binding domain by fusing the Bacillus subtilis xyn gene fragment to the 5'-end of the Cellulomonas fimi cenA, overexpression in Escherichia coli |
3.2.1.4 | Ag-EGase III is expressed as a 47000 Da polypeptide in baculovirus-infected insect Sf9 cells |
3.2.1.4 | by removing some of the C-terminal sequence of the ORF of the endoglucanase (PH1171), two types of recombinant proteins are expressed from one ORF, using Escherichia coli |
3.2.1.4 | catalytic core domain expressed in Escherichia coli |
3.2.1.4 | Cel6A production in Escherichia coli and the DB-Cel6A fusions are inserted by particle bombardment method into the tobacco (Nicotiana tabacum cv. Samsun) chloroplast genome for protein expression |
3.2.1.4 | CenA, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha |
3.2.1.4 | chimeric enzymes between the Bacillus subtilis cellulase and an alkalophilic Bacillus cellulase, expression in Escherichia coli |
3.2.1.4 | cloned and overexpressed in both native form (nCfEG) and C-terminal His-tagged form (tCfEG) in Escherichia coli |