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1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O
D-glucuronic acid + ?
2-O-alpha-(4-O-methyl-alpha-D-glucopyranosyluronic acid)-D-xylose + H2O
4-O-methylglucuronic acid + D-xylose
-
-
-
-
?
2-O-alpha-(4-O-methyl-alpha-D-glucopyranosyluronic)-D-xylobiose + H2O
4-O-methylglucuronic acid + D-xylobiose
-
i.e. aldotriouronic acid
-
-
?
2-O-alpha-(4-O-methyl-alpha-D-glucuronosyl)-xylotriose + H2O
4-O-methyl-alpha-D-glucuronic acid + xylotriose
2-O-alpha-D-glucopyranosyluronic acid-D-xylose + H2O
alpha-D-glucopyranosyluronic acid + D-xylose
-
-
-
-
?
2-O-alpha-D-glucuronosyl-D-xylose + H2O
D-glucuronate + D-xylose
-
-
-
-
?
3-O-alpha-D-glucuronosyl-D-xylose + H2O
D-glucuronate + D-xylose
-
-
-
-
?
4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylopyranoside + 4-O-methyl-alpha-D-glucopyranose
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside + H2O
4-nitrophenyl-D-xylopyranoside + 4-O-methyl-alpha-D-glucuronate
-
-
-
-
?
4-O-alpha-D-glucuronosyl-D-xylose + H2O
D-glucuronate + D-xylose
-
-
-
-
?
4-O-Me-GlcAalpha(1-2)Xylbeta(1-4)Xylbeta(1-4)Xyl + H2O
4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
from beechwood, de-estrification of the methyl ester of MeGlcA with glucuronoyl esterase
de-estrification of the methyl ester of MeGlcA with glucuronoyl esterase
-
?
4-O-Me-GlcAalpha(1-2)Xylbeta(1-4)Xylbeta(1-4)[4-O-Me-GlcAalpha(1-2)]Xylbeta(1-4)Xyl + H2O
4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)[4-O-Me-GlcAalpha(1-2)]Xylbeta(1-4)Xyl
hydrolysis product of endoxylanases on glucuronoxylan
de-estrification of the methyl ester of MeGlcA with glucuronoyl esterase
-
?
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-(beta-D-xylopyranosyl-(1->4))n-beta-D-xylopyranose + H2O
4-O-methyl-alpha-D-glucopyranose + beta-D-xylopyranosyl-(1->4)-(beta-D-xylopyranosyl-(1->4))n-beta-D-xylopyranose
-
-
-
ir
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
4-O-methyl-alpha-D-glucopyranose + 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
4-O-methyl-alpha-D-glucopyranose + beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose
-
-
-
ir
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
4-O-methyl-alpha-D-glucopyranose + beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose
-
-
-
ir
4-O-methyl-alpha-D-glucopyranuronosyl-(1-2)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O
4-O-methyl-alpha-D-glucuronic acid + beta-D-xylopyranose
-
-
-
-
?
4-O-methyl-alpha-D-glucopyranuronosyl-(1-2)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O
4-O-methyl-alpha-D-glucuronic acid + beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
-
-
-
?
4-O-methyl-alpha-D-glucuronosyl-xyloheptaose + H2O
4-O-methyl-alpha-D-glucuronate + xyloheptaose
-
-
-
-
?
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
?
4-O-methyl-D-glucuronosyl-xylitol + H2O
4-O-methyl-D-glucuronate + xylitol
4-O-methyl-D-glucuronosylxylan + H2O
4-O-methyl-D-glucuronate + xylan
4-O-methyl-D-glucuronoxylan + H2O
4-O-methyl-D-glucuronic acid + xylan
-
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
4-O-methyl-glucuronosyl-xylohexaose + H2O
4-O-methyl-glucuronate + xylohexaose
4-O-methyl-glucuronosyl-xylopentaose + H2O
4-O-methyl-glucuronate + xylopentaose
4-O-methyl-glucuronosyl-xylose + H2O
4-O-methyl-glucuronate + xylose
4-O-methyl-glucuronosyl-xylotetraose + H2O
4-O-methyl-glucuronate + xylotetraose
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
acetylated glucuronoxylan + H2O
?
-
-
-
-
?
aldobiouronic acid + H2O
?
aldobiouroniuc acid + H2O
?
aldohexaouronic acid + H2O
?
aldopentaouronic acid + H2O
4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
-
-
-
-
?
aldopentaouronic acid + H2O
4-O-methylglucuronic acid + ?
-
high activity
-
-
?
aldopentaouronic acid + H2O
?
aldopentauronic acid + H2O
4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
aldotetraouronic acid + H2O
4-O-methylglucuronic acid + ?
-
highest activity
-
-
?
aldotetraouronic acid + H2O
?
aldotetrauronic acid + H2O
?
aldotriouronic acid + H2O
4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
aldouronic acid + H2O
D-xylose + 4-O-methyl-D-glucuronic acid
amylouronate + H2O
D-glucuronate + ?
beechwood glucuronoxylan + H2O
?
beechwood xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
-
-
-
?
beechwood xylan + H2O
4-O-methylglucuronic acid + ?
-
low activity
-
-
?
beechwood xylan + H2O
4-O-methylglucuronoxylan + ?
-
-
-
?
beechwood xylan + H2O
?
-
-
-
?
benzyl 4-O-alpha-D-glucuronosyl-beta-D-glucoside + H2O
?
-
-
-
-
?
beta-D-glucuronyl alpha-D-glucuronic acid + H2O
D-glucuronate + alpha-D-glucuronic acid
birchwood xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid
-
-
-
?
birchwood xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
birchwood xylan + H2O
4-O-methylglucuronic acid + ?
-
very low activity
-
-
?
birchwood xylan + H2O
4-O-methylglucuronoxylan + ?
-
-
-
?
GlcAalpha(1-2)Xylbeta(1-4)Xylbeta(1-4)[GlcAalpha(1-2)]Xylbeta(1-4)Xyl + H2O
alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)[GlcAalpha(1-2)]Xylbeta(1-4)Xyl
generated from the action of endoxylanases on polysaccharides
de-estrification of the methyl ester of MeGlcA with glucuronoyl esterase
-
?
glucuronosyl-xylotriose + H2O
glucuronate + xylotriose
glucuronoxylan + H2O
4-O-methyl-D-glucuronic acid + ?
glycyrrhizin + H2O
glycyrrhetinic acid + 2 D-glucuronate
hexenuronosyl xylotriose + H2O
hexenuronic acid + xylotriose
O-alpha-D-glucopyranosyluronic acid-alpha-D-glucopyranosiduronic acid + H2O
alpha-D-glucopyranosyluronic acid + alpha-D-glucopyranosiduronic acid
-
-
-
-
?
O-alpha-D-glucosyluronic acid alpha-D-glucosiduronic acid
D-glucuronic acid
-
the immobilized enzyme completely hydrolyzes 100 mM of the substrate
-
-
?
O-alpha-D-glucosyluronic acid alpha-D-glucosiduronic acid + H2O
alpha-D-glucosiduronic acid + beta-D-glucosiduronic acid
-
-
alpha-D-glucosiduronic acid and beta-D-glucosiduronic acid are produced in an equimolar ratio
-
?
O-alpha-glucuronosyl alpha-D-glucuronide + H2O
D-glucuronate
-
-
-
-
?
oat spelt xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
oat spelt xylan + H2O
4-O-methylglucuronic acid + ?
-
negligible activity
-
-
?
p-nitrophenyl alpha-D-glucuronide + H2O
p-nitrophenol + D-glucuronate
-
-
-
-
?
p-nitrophenyl alpha-D-glucuronopyranoside + H2O
p-nitrophenol + D-glucuronate
p-nitrophenyl-alpha-D-glucuronopyranoside + H2O
p-nitrophenol + D-glucuronate
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronate
reduced aldopentaouronic acid + H2O
4-O-methyl-D-glucuronic acid + ?
-
-
-
-
?
spruce arabinoxylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
spruce gluconoarabinoxylan + H2O
4-O-methyl-D-glucuronic acid + ?
-
-
-
-
?
additional information
?
-
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O
D-glucuronic acid + ?
hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds
-
-
?
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O
D-glucuronic acid + ?
hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds
-
-
?
2-O-alpha-(4-O-methyl-alpha-D-glucuronosyl)-xylotriose + H2O
4-O-methyl-alpha-D-glucuronic acid + xylotriose
-
i.e. aldotetraouronic acid
-
-
?
2-O-alpha-(4-O-methyl-alpha-D-glucuronosyl)-xylotriose + H2O
4-O-methyl-alpha-D-glucuronic acid + xylotriose
-
i.e. aldotetraouronic acid
-
-
?
4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylopyranoside + 4-O-methyl-alpha-D-glucopyranose
-
-
-
-
?
4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylopyranoside + 4-O-methyl-alpha-D-glucopyranose
-
-
-
-
?
4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylopyranoside + 4-O-methyl-alpha-D-glucopyranose
-
-
-
-
?
4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylopyranoside + 4-O-methyl-alpha-D-glucopyranose
-
-
-
-
?
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
4-O-methyl-alpha-D-glucopyranose + 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose
-
-
-
ir
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
4-O-methyl-alpha-D-glucopyranose + 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose
-
-
-
ir
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
?
-
-
-
?
4-O-methyl-beta-D-glucopyranuetaronosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
?
-
-
-
?
4-O-methyl-D-glucuronosyl-xylitol + H2O
4-O-methyl-D-glucuronate + xylitol
-
-
-
-
?
4-O-methyl-D-glucuronosyl-xylitol + H2O
4-O-methyl-D-glucuronate + xylitol
-
-
-
-
?
4-O-methyl-D-glucuronosyl-xylitol + H2O
4-O-methyl-D-glucuronate + xylitol
-
-
-
-
?
4-O-methyl-D-glucuronosyl-xylitol + H2O
4-O-methyl-D-glucuronate + xylitol
Polyporus versicolor
-
-
-
-
?
4-O-methyl-D-glucuronosyl-xylitol + H2O
4-O-methyl-D-glucuronate + xylitol
-
-
-
-
?
4-O-methyl-D-glucuronosyl-xylitol + H2O
4-O-methyl-D-glucuronate + xylitol
Tyromyces palustris
-
-
-
-
?
4-O-methyl-D-glucuronosylxylan + H2O
4-O-methyl-D-glucuronate + xylan
-
-
-
-
?
4-O-methyl-D-glucuronosylxylan + H2O
4-O-methyl-D-glucuronate + xylan
-
-
-
-
?
4-O-methyl-D-glucuronosylxylan + H2O
4-O-methyl-D-glucuronate + xylan
-
at 52% of the activity with 4-O-methyl-glucuronosyl-xylobiose
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylobiose + H2O
4-O-methyl-glucuronate + xylobiose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylohexaose + H2O
4-O-methyl-glucuronate + xylohexaose
-
at 53% of the activity with 4-O-methyl-glucuronosyl-xylobiose
-
-
?
4-O-methyl-glucuronosyl-xylohexaose + H2O
4-O-methyl-glucuronate + xylohexaose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylopentaose + H2O
4-O-methyl-glucuronate + xylopentaose
-
at 50% of the activity with 4-O-methyl-glucuronosyl-xylobiose
-
-
?
4-O-methyl-glucuronosyl-xylopentaose + H2O
4-O-methyl-glucuronate + xylopentaose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylose + H2O
4-O-methyl-glucuronate + xylose
-
no activity
-
-
?
4-O-methyl-glucuronosyl-xylose + H2O
4-O-methyl-glucuronate + xylose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylose + H2O
4-O-methyl-glucuronate + xylose
-
no activity
-
-
?
4-O-methyl-glucuronosyl-xylose + H2O
4-O-methyl-glucuronate + xylose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylose + H2O
4-O-methyl-glucuronate + xylose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylose + H2O
4-O-methyl-glucuronate + xylose
-
96% of the activity with 4-O-methyl-glucuronosyl-xylobiose
-
-
?
4-O-methyl-glucuronosyl-xylose + H2O
4-O-methyl-glucuronate + xylose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylose + H2O
4-O-methyl-glucuronate + xylose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotetraose + H2O
4-O-methyl-glucuronate + xylotetraose
-
at 96% of the activity with 4-O-methyl-glucuronosyl-xylobiose
-
-
?
4-O-methyl-glucuronosyl-xylotetraose + H2O
4-O-methyl-glucuronate + xylotetraose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotetraose + H2O
4-O-methyl-glucuronate + xylotetraose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotetraose + H2O
4-O-methyl-glucuronate + xylotetraose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotetraose + H2O
4-O-methyl-glucuronate + xylotetraose
-
at 96% of the activity with 4-O-methyl-glucuronosyl-xylobiose
-
-
?
4-O-methyl-glucuronosyl-xylotetraose + H2O
4-O-methyl-glucuronate + xylotetraose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotetraose + H2O
4-O-methyl-glucuronate + xylotetraose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
at 99% of the activity with 4-O-methyl-glucuronosyl-xylobiose
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
at 96% of the activity 4-O-methyl-glucuronosyl-xylobiose
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
4-O-methyl-glucuronosyl-xylotriose + H2O
4-O-methyl-glucuronate + xylotriose
-
-
-
-
?
aldobiouronic acid + H2O
?
-
-
-
-
?
aldobiouronic acid + H2O
?
-
-
-
?
aldobiouronic acid + H2O
?
-
-
-
-
?
aldobiouroniuc acid + H2O
?
-
-
-
-
?
aldobiouroniuc acid + H2O
?
-
-
-
-
?
aldohexaouronic acid + H2O
?
-
-
-
-
?
aldohexaouronic acid + H2O
?
-
best substrate
-
-
?
aldohexaouronic acid + H2O
?
-
best substrate
-
-
?
aldopentaouronic acid + H2O
?
-
-
-
-
?
aldopentaouronic acid + H2O
?
-
-
-
-
?
aldopentaouronic acid + H2O
?
-
-
-
-
?
aldopentaouronic acid + H2O
?
-
-
-
?
aldopentaouronic acid + H2O
?
-
-
-
-
?
aldopentaouronic acid + H2O
?
-
-
-
-
?
aldopentauronic acid + H2O
4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
-
-
-
-
?
aldopentauronic acid + H2O
4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
-
-
-
-
?
aldopentauronic acid + H2O
4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
-
-
-
-
?
aldopentauronic acid + H2O
4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
i.e. 2''-O-alpha-(4-O-methyl-alpha-D-glucuronosyl)-xylotetraose
-
-
?
aldotetraouronic acid + H2O
?
-
-
-
-
?
aldotetraouronic acid + H2O
?
-
-
-
?
aldotetraouronic acid + H2O
?
-
-
-
-
?
aldotetraouronic acid + H2O
?
-
-
-
-
?
aldotetraouronic acid + H2O
?
-
-
-
-
?
aldotetraouronic acid + H2O
?
-
-
-
-
?
aldotetrauronic acid + H2O
?
-
-
-
-
?
aldotetrauronic acid + H2O
?
-
best substrate
-
-
?
aldotetrauronic acid + H2O
?
-
-
-
-
?
aldotetrauronic acid + H2O
?
-
-
-
-
?
aldotriouronic acid + H2O
4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
-
-
-
?
aldotriouronic acid + H2O
4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
-
-
?
aldotriouronic acid + H2O
4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
-
-
-
?
aldotriouronic acid + H2O
4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
-
-
-
?
aldotriouronic acid + H2O
4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
-
-
-
?
aldouronic acid + H2O
D-xylose + 4-O-methyl-D-glucuronic acid
mixtures of aldobiouronic, aldottriouronic, aldotetraouronic, and aldopentaouronic acid, glucuronic acid groups attached via 1,2-linkage to xylose or non-reducing terminal residue of xylose-oligosaccharides
direct product of aldobiouronic acid
-
?
aldouronic acid + H2O
D-xylose + 4-O-methyl-D-glucuronic acid
mixtures of aldobiouronic, aldottriouronic, aldotetraouronic, and aldopentaouronic acid, glucuronic acid groups attached via 1,2-linkage to xylose or non-reducing terminal residue of xylose-oligosaccharides
direct product of aldobiouronic acid
-
?
amylouronate + H2O
D-glucuronate + ?
-
AUH-I is highly specific to amylouronate, amylouronate is alpha-(1,4)-linked polyglucuronic acid sodium salt or alpha-(1,4)-linked glucuronan, AUH-I hydrolyses amylouronate exolytically
-
-
?
amylouronate + H2O
D-glucuronate + ?
-
AUH-I is highly specific to amylouronate, amylouronate is alpha-(1,4)-linked polyglucuronic acid sodium salt or alpha-(1,4)-linked glucuronan, AUH-I hydrolyses amylouronate exolytically
-
-
?
beechwood glucuronoxylan + H2O
?
-
-
-
-
?
beechwood glucuronoxylan + H2O
?
-
-
-
-
?
beechwood glucuronoxylan + H2O
?
-
-
-
-
?
beta-D-glucuronyl alpha-D-glucuronic acid + H2O
D-glucuronate + alpha-D-glucuronic acid
-
-
-
-
?
beta-D-glucuronyl alpha-D-glucuronic acid + H2O
D-glucuronate + alpha-D-glucuronic acid
-
-
-
-
?
birchwood xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
-
-
-
?
birchwood xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
-
-
-
?
birchwood xylan + H2O
?
-
-
-
?
birchwood xylan + H2O
?
-
-
-
-
?
birchwood xylan + H2O
?
-
-
release of reduced sugar
-
?
glucuronosyl-xylotriose + H2O
glucuronate + xylotriose
-
-
-
-
?
glucuronosyl-xylotriose + H2O
glucuronate + xylotriose
-
-
-
-
?
glucuronoxylan + H2O
4-O-methyl-D-glucuronic acid + ?
-
-
-
-
?
glucuronoxylan + H2O
4-O-methyl-D-glucuronic acid + ?
-
-
-
-
?
glycyrrhizin + H2O
glycyrrhetinic acid + 2 D-glucuronate
-
-
-
-
?
glycyrrhizin + H2O
glycyrrhetinic acid + 2 D-glucuronate
-
-
-
-
?
hexenuronosyl xylotriose + H2O
hexenuronic acid + xylotriose
-
-
-
?
hexenuronosyl xylotriose + H2O
hexenuronic acid + xylotriose
-
-
-
?
oat spelt xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
-
1% of the activity with birchwood xylan
-
?
oat spelt xylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
-
1% of the activity with birchwood xylan
-
?
p-nitrophenyl alpha-D-glucuronopyranoside + H2O
p-nitrophenol + D-glucuronate
-
-
-
?
p-nitrophenyl alpha-D-glucuronopyranoside + H2O
p-nitrophenol + D-glucuronate
-
-
-
?
p-nitrophenyl-alpha-D-glucuronopyranoside + H2O
p-nitrophenol + D-glucuronate
-
-
-
-
?
p-nitrophenyl-alpha-D-glucuronopyranoside + H2O
p-nitrophenol + D-glucuronate
-
-
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronate
-
3% of the activity with 18beta-glycyrrhetinic acid beta-D-glucuronyl alpha-D-glucuronic acid
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronate
-
3% of the activity with 18beta-glycyrrhetinic acid beta-D-glucuronyl alpha-D-glucuronic acid
-
-
?
spruce arabinoxylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
-
37% of the activity with birchwood xylan
-
?
spruce arabinoxylan + H2O
4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
-
37% of the activity with birchwood xylan
-
?
additional information
?
-
-
enzyme is involved in hydrolysis of wood xylans
-
-
?
additional information
?
-
-
alpha-glucuronidases act on xylan side-chains and release methyl glucuronic acid residues. The enzyme is active on xylooligosaccharides generated from birchwood glucuronoxylan by a family 10 endoxylanase and on a mixture of aldouronic acids. No activity with birchwood glucuronoxylan
-
-
?
additional information
?
-
-
alpha-glucuronidases act on xylan side-chains and release methyl glucuronic acid residues. The enzyme is active on xylooligosaccharides generated from birchwood glucuronoxylan by a family 10 endoxylanase and on a mixture of aldouronic acids. No activity with birchwood glucuronoxylan
-
-
?
additional information
?
-
-
no hydrolysis of O-alpha-D-glucosyluronic acid alpha-D-glucoside, O-alpha-D-glucosyluronic acid beta-D-glucosiduronic acid, O-alpha-D-glucosyluronic acid-(1,2)-beta-D-fructosiduronic acid, p-nitrophenyl-O-alpha-D-glucosiduronic acid, methyl-O-alpha-D-glucosiduronic acid, or 6-O-alpha-(4-O-alpha-D-glucosyluronic acid)-D-glucosyl-beta-cyclodextrine
-
-
?
additional information
?
-
the enzyme hydrolyzes methyl-alpha-D-glucuronic acid side chains from the internal regions of xylan. The enzyme is required to undergo a substantial conformational change to form a productive Michaelis complex with glucuronoxylan
-
-
?
additional information
?
-
-
the enzyme hydrolyzes methyl-alpha-D-glucuronic acid side chains from the internal regions of xylan. The enzyme is required to undergo a substantial conformational change to form a productive Michaelis complex with glucuronoxylan
-
-
?
additional information
?
-
hydrolysis of the glucurono-xylooligosaccharides derived from mature Arabidopsis thaliana wild-type and gux1gux2 stems as well as wild-type willow, barley, sugar cane, and Miscanthus stems, BoAgu115A is an alpha-glucuronidase that targets the uronic acids that decorate xylans
-
-
?
additional information
?
-
-
hydrolysis of the glucurono-xylooligosaccharides derived from mature Arabidopsis thaliana wild-type and gux1gux2 stems as well as wild-type willow, barley, sugar cane, and Miscanthus stems, BoAgu115A is an alpha-glucuronidase that targets the uronic acids that decorate xylans
-
-
?
additional information
?
-
-
no activity with baicalin
-
-
?
additional information
?
-
-
no activity with baicalin
-
-
?
additional information
?
-
the role of the glucuronidase, in combination with cell-associated xylanases could be to hydrolyze decorated xylooligosaccharides, generated by extracellular hemicellulases, to xylose and 4-O-methyl-D-glucuronic acid, enabling the pseudomonad to preferentially utilize the sugars derived from these polymers
-
-
?
additional information
?
-
-
the role of the glucuronidase, in combination with cell-associated xylanases could be to hydrolyze decorated xylooligosaccharides, generated by extracellular hemicellulases, to xylose and 4-O-methyl-D-glucuronic acid, enabling the pseudomonad to preferentially utilize the sugars derived from these polymers
-
-
?
additional information
?
-
-
the enzyme is involved in hydrolysis of xylan. Presence of endoxylanase is critical for efficient alpha-glucuronidase activity, and efficient alpha-glucuronidase activity is essential for the complete hydrolysis of intact xylan
-
-
?
additional information
?
-
the enzyme shows highly specific activity for methylglucoronate side groups of aldouronic acids, while no activity is observed with birchwood xylan, 4-O-methyl-D-glucurono-D-xylan, or 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
additional information
?
-
the enzyme shows highly specific activity for methylglucoronate side groups of aldouronic acids, while no activity is observed with birchwood xylan, 4-O-methyl-D-glucurono-D-xylan, or 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
additional information
?
-
-
the enzyme shows highly specific activity for methylglucoronate side groups of aldouronic acids, while no activity is observed with birchwood xylan, 4-O-methyl-D-glucurono-D-xylan, or 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
additional information
?
-
isoform GH115 is able to release 4-O-methylglucuronoxylan from both polymeric 4-O-methylglucuronoxylan and 4-O-methylglucurono-xylo-oligosaccharides. GH115 cleaves 4-O-ethylglucuronoxylan from both internal and non-reducing end xylosyl residues, with the exception of 4-O-methylglucuronate attached to the non-reducing end of a xylotriose oligosaccharide
-
-
?
additional information
?
-
isoform GH115 is able to release 4-O-methylglucuronoxylan from both polymeric 4-O-methylglucuronoxylan and 4-O-methylglucurono-xylo-oligosaccharides. GH115 cleaves 4-O-ethylglucuronoxylan from both internal and non-reducing end xylosyl residues, with the exception of 4-O-methylglucuronate attached to the non-reducing end of a xylotriose oligosaccharide
-
-
?
additional information
?
-
-
isoform GH115 is able to release 4-O-methylglucuronoxylan from both polymeric 4-O-methylglucuronoxylan and 4-O-methylglucurono-xylo-oligosaccharides. GH115 cleaves 4-O-ethylglucuronoxylan from both internal and non-reducing end xylosyl residues, with the exception of 4-O-methylglucuronate attached to the non-reducing end of a xylotriose oligosaccharide
-
-
?
additional information
?
-
isoform GH67 is able to release O-methylglucuronoxylan from O-methylglucuronoxylan-substituted xylo-oligosaccharides (UAmeXOS), but only the O-methylglucuronoxylan linked to the non-reducing end xylosyl residue is cleaved
-
-
?
additional information
?
-
isoform GH67 is able to release O-methylglucuronoxylan from O-methylglucuronoxylan-substituted xylo-oligosaccharides (UAmeXOS), but only the O-methylglucuronoxylan linked to the non-reducing end xylosyl residue is cleaved
-
-
?
additional information
?
-
-
isoform GH67 is able to release O-methylglucuronoxylan from O-methylglucuronoxylan-substituted xylo-oligosaccharides (UAmeXOS), but only the O-methylglucuronoxylan linked to the non-reducing end xylosyl residue is cleaved
-
-
?
additional information
?
-
the enzyme shows activity toward glucuronoxylan and oligomers thereof with preference toward 4-O-methyl glucopyranosyluronic acid linked to internal xylopyranosyl residues
-
-
?
additional information
?
-
-
the enzyme shows activity toward glucuronoxylan and oligomers thereof with preference toward 4-O-methyl glucopyranosyluronic acid linked to internal xylopyranosyl residues
-
-
?
additional information
?
-
the enzyme shows activity toward glucuronoxylan and oligomers thereof with preference toward 4-O-methyl glucopyranosyluronic acid linked to internal xylopyranosyl residues
-
-
?
additional information
?
-
-
no activity with p-nitrophenyl-alpha-D-glucuronoside
-
-
?
additional information
?
-
-
important enzyme for the utilization of substituted xylans
-
-
?
additional information
?
-
-
enzyme is induced by growth on beech wood or birch wood
-
-
?
additional information
?
-
-
important enzyme for the utilization of substituted xylans
-
-
?
additional information
?
-
-
enzyme is induced by growth on beech wood or birch wood
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
the three enzymes, xylanase, alpha-D-glucuronidase, and beta-glucosidase, together are responsible for complete degradation of larchwood xylan to xylose and 4-O-methyl-alpha-D-glucuronic acid
-
-
?
additional information
?
-
-
no activity with p-nitrophenyl-alpha-D-glucuronoside
-
-
?
additional information
?
-
-
enzyme prefers low-molecular-weight xylooligomers as substrates. The enzyme acts almost exclusively on the bond between the terminal xylose at the nonreducing end of the xylose chain and the methyl glucuronic acid attached to it
-
-
?
additional information
?
-
-
enzyme prefers low-molecular-weight xylooligomers as substrates. The enzyme acts almost exclusively on the bond between the terminal xylose at the nonreducing end of the xylose chain and the methyl glucuronic acid attached to it
-
-
?
additional information
?
-
-
the enzyme functions synergistically with a GH10 endoxylanase enzyme to hydrolyze the substrate
-
-
?
additional information
?
-
-
the enzyme functions synergistically with xylanase in the hydrolysis of xylan substrate. The main products of the hydrolysis reaction are xylotriose, xylotetraose, and xylopentaose
-
-
?
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D192A
site-directed mutagenesis, the mutation has no effect on the enzyme activity
D206A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
D332A
site-directed mutagenesis, inactive mutant
D396N
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
D478A
site-directed mutagenesis, the mutation has no effect on the enzyme activity
E162A
site-directed mutagenesis, the mutation has no effect on the enzyme activity
E375A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
E782A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
E785A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H275A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
H275A/H422A
site-directed mutagenesis, inactive mutant
H422A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
K374A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
N205A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
N398A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
N462A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R328A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
W169A
site-directed mutagenesis, the mutation has no effect on the enzyme activity
W249A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
Y373A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
Y420A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
Y425A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y788A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y792A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
D365A
-
activity is 0.0001% of wild-type activity
D365C
-
activity is 0.0001% of wild-type activity
E292C
-
activity is 0.0001% of wild-type activity
E393A
-
activity is 0.0001% of wild-type activity
E393C
-
activity is22% of wild-type activity
K288A
-
kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.003% of wild-type value
K360A
-
kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.019% of wild-type value
R325A
-
kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.000096% of wild-type value
V210A
-
kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 38% of wild-type value
V210G
-
kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is identical to wild-type value
V210N
-
kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 9.4% of wild-type value
V210S
-
kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 4.3% of wild-type value
W160A
-
kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.0064% of wild-type value
W543A
-
kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.069% of wild-type value
D274A
-
as active as wild-type enzyme
D364A
-
0.000015% of wild-type activity
D364A/E392C
-
0.0000004% of wild-type activity
E158N
-
0.07% of wild-type activity
E285N
-
0.0002% of wild-type activity
E386Q
-
0.07% of wild-type activity
E392C
-
0.00002% of wild-type activity
E510A
-
20% of wild-type activity
W328E/R329T
-
activity of the monomeric mutant enzyme is significantly lower than activity of dimeric wild-type enzyme
W328E/R329T/R665N
-
activity of the monomeric mutant enzyme is significantly lower than activity of dimeric wild-type enzyme, melting temperature is 0.5°C lower than. OPtimal temperature is around 35°C, compared to 65° for the wild-type enzym
D274A
-
as active as wild-type enzyme
-
D364A
-
0.000015% of wild-type activity
-
E158N
-
0.07% of wild-type activity
-
E510A
-
20% of wild-type activity
-
W328E/R329T
-
activity of the monomeric mutant enzyme is significantly lower than activity of dimeric wild-type enzyme
-
W328E/R329T/R665N
-
activity of the monomeric mutant enzyme is significantly lower than activity of dimeric wild-type enzyme, melting temperature is 0.5°C lower than. OPtimal temperature is around 35°C, compared to 65° for the wild-type enzym
-
D215A
15fold decrease in catalytic efficiency
E216A
complete loss of activtiy
E381A
330fold decrease in catalytic efficiency
F696A
3-5fold decrease in kcat value
R331A
26000fold decrease in catalytic efficiency
W689A
3-5fold decrease in kcat value
W773A
3-5fold decrease in kcat value
E216A
-
complete loss of activtiy
-
E381A
-
330fold decrease in catalytic efficiency
-
W773A
-
3-5fold decrease in kcat value
-
E292A
-
inactive mutant enzyme
E292A
-
activity is 0.0001% of wild-type activity
D335A
complete loss of activtiy
D335A
weak binding of substrate
D335A
-
complete loss of activtiy
-
D335A
-
weak binding of substrate
-
additional information
deletion of C-terminal residues 1-526, 1-639, and 1-665 results in inactive enzyme mutants
additional information
-
deletion of C-terminal residues 1-526, 1-639, and 1-665 results in inactive enzyme mutants
additional information
-
truncated forms of the enzyme, lacking either 126 amino acids from its N-terminus or 81 amino acids from its C-terminus, exhibit low residual activity
additional information
-
truncated forms of the enzyme, lacking either 126 amino acids from its N-terminus or 81 amino acids from its C-terminus, exhibit low residual activity
-
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Uchida, H.; Nanri, T.; Kawabata, Y.; Kusakabe, I.; Murakami, K.
Purification and characterization of intracellular alpha-glucuronidase from Aspergillus niger 5-16
Biosci. Biotechnol. Biochem.
56
1608-1615
1992
Aspergillus niger, Aspergillus niger 5-16
-
brenda
Puls, J.
alpha-Glucuronidases in the hydrolysis of wood xylans
Xylans and Xylanases (Visser, J. , et al. , Eds. ) Elsevier, Amsterdam
213-224
1992
Agaricus bisporus, Aspergillus awamori, Hypomyces rosellus, Laetiporus sulphureus, Pleurotus ostreatus, Polyporus versicolor, Streptomyces olivochromogenes, Thermoascus aurantiacus, Trichoderma reesei, Tyromyces palustris
-
brenda
Kim, D.H.; Jang, H.S.; Lee, S.W.
Bacteroides J-37, a human intestinal bacterium, produces alpha-glucuronidase
Biol. Pharm. Bull.
20
834-837
1997
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Helix pomatia
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Aspergillus tubingensis, Aureobasidium pullulans, Trichoderma reesei, Trichoderma reesei RUT C-30
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Geobacillus stearothermophilus
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Thermotoga maritima (P96105), Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 (P96105)
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Cellvibrio japonicus
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Rasamsonia emersonii, Rasamsonia emersonii CBS 814.70
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Scheffersomyces stipitis (A3LY17), Scheffersomyces stipitis
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Paenibacillus sp., Paenibacillus sp. TH501b
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Streptomyces pristinaespiralis (B5H8Y8)
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Aspergillus fumigatus, Aspergillus fumigatus A1163
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Bacteroides ovatus (A7M022), Bacteroides ovatus
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Paenibacillus sp. (C6D678)
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A GH115 alpha-glucuronidase from Schizophyllum commune contributes to the synergistic enzymatic deconstruction of softwood glucuronoarabinoxylan
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002
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Schizophyllum commune
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The two Rasamsonia emersonii alpha-glucuronidases, ReGH67 and ReGH115, show a different mode-of-action towards glucuronoxylan and glucuronoxylo-oligosaccharides
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105
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Rasamsonia emersonii (A0A168KIJ9), Rasamsonia emersonii (Q8X211), Rasamsonia emersonii
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Schizophyllum commune
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The GH67 alpha-glucuronidase of Paenibacillus curdlanolyticus B-6 removes hexenuronic acid groups and facilitates biodegradation of the model xylooligosaccharide hexenuronosyl xylotriose
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Paenibacillus curdlanolyticus (A0A0A0V7L5), Paenibacillus curdlanolyticus B-6 (A0A0A0V7L5), Paenibacillus curdlanolyticus B-6
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Biochemical and structural characterization of a five-domain GH115 alpha-Glucuronidase from the marine bacterium Saccharophagus degradans 2-40T
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Saccharophagus degradans (Q21JW4), Saccharophagus degradans, Saccharophagus degradans DSM 17024 (Q21JW4)
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Scheffersomyces stipitis
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brenda