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Literature summary for 3.2.1.139 extracted from

  • Rhee, M.; Sawhney, N.; Kim, Y.; Rhee, H.; Hurlbert, J.; St. John, F.; Nong, G.; Rice, J.; Preston, J.
    GH115 alpha-glucuronidase and GH11 xylanase from Paenibacillus sp. JDR-2: potential roles in processing glucuronoxylans (2016), Appl. Microbiol. Biotechnol., 101, 1465-1476.
    View publication on PubMed
Search for more literature for 3.2.1.139

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure. The likely catalytic residues are Asp303 (nucleophile) and Glu350 (proton donor). An arginine (Arg299 in Pjdr2) is conserved in all the Agu115 enzymes considered. The xylose-binding cleft bounded by Trp249 and Val426 in Agu115A is predicted to be lined by Trp272 and Met401 in the Agu115A model Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. C6D678
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.9
-
 substrate 4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose, pH not specified in the publication, temperature not specified in the publication Paenibacillus sp.
16.3
-
 substrate 4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose, pH not specified in the publication, temperature not specified in the publication Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-(beta-D-xylopyranosyl-(1->4))n-beta-D-xylopyranose + H2O
-
 Paenibacillus sp. 4-O-methyl-alpha-D-glucopyranose + beta-D-xylopyranosyl-(1->4)-(beta-D-xylopyranosyl-(1->4))n-beta-D-xylopyranose
-
 ir
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
-
 Paenibacillus sp. 4-O-methyl-alpha-D-glucopyranose + beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose
-
 ir
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose + H2O
-
 Paenibacillus sp. 4-O-methyl-alpha-D-glucopyranose + beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose
-
 ir

Synonyms

Synonyms Comment Organism
Agu115A
-
 Paenibacillus sp.
Pjdr2_5977
-
 Paenibacillus sp.

Expression

Organism Comment Expression
Paenibacillus sp. enhanced expression during growth on xylans up

General Information

General Information Comment Organism
physiological function the enzyme catalyzes removal of 4-O-methylglucuronate residues alpha-1,2 linked to internal xylose residues in oligoxylosides generated by GH11 and GH30 xylanases and releases methylglucuronate from polymeric methylglucuronoxylan Paenibacillus sp.